A method for preparing live attenuated mumps vaccine

Abstract

The invention relates to a method for preparing live attenuated mumps vaccine, which belongs to the technical field of biomedicine. The invention provides a method for preparing a live attenuated mumps vaccine, the method comprising a clarification step using continuous flow centrifugation under the conditions of a fixed centrifugal force of 4000-14000g and a sample loading speed of 400-1500mL / min , to clarify the virus harvest liquid of the attenuated mumps virus strain; compared with the clarification methods (membrane filtration and silk cloth filtration) of the existing mumps live attenuated vaccine, the clarification effect of the method is more stable and the degree of automation is higher Higher, easier to operate, easier to achieve aseptic operation, which helps to reduce the quality and safety risks of live attenuated mumps vaccines, and the method has the best removal effect on residual cell debris in the virus harvesting liquid, and has the best effect on the virus The impact of infectious mumps virus titer in the harvest fluid is minimal, which is helpful to obtain live attenuated mumps vaccine with high virus titer.

Description

technical field [0001] The invention relates to a method for preparing live attenuated mumps vaccine, which belongs to the technical field of biomedicine. Background technique [0002] Mumps is an acute infectious disease caused by mumps virus (MuV), and is a common respiratory infectious disease in children and adolescents. The main clinical symptom is swelling and pain of the parotid gland, and sometimes acute inflammation or systemic inflammation such as viral encephalitis, orchitis, pancreatitis or oophoritis can also be caused. [0003] MuV is mainly transmitted through saliva, saliva-contaminated items, and air droplets. Mumps is one of the world's prevalent infectious diseases, with a very wide regional distribution. The prevalence and outbreak of mumps have no obvious seasonal and regional characteristics, and are not affected by climate and other factors, but the number of cases in winter and spring is more than that in summer. At this stage, the main measure to ...

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Problems solved by technology

Among them, there are many defects in the spinner bottle process. For example, the cell breeding area of ​​a single spinner bottle is limited, large-scale production requires a large number of spinner bottles, and the risk of aseptic control is higher; affected by factors such as operating methods, liquid leakage may occur, resulting in large-scale production. The effect of the method cannot be guaranteed; the space used in the plant is larger, and the harvest liquid of the same volume requires more space than the cell factory; the cleaning and sterilization of spinner bottles requires a lot of labor, and it is difficult to automate operations such as liquid replacement

At the same time, the clarification method of the harvest liquid of the attenuated mumps virus strain also has many defects that affect its clarification effect and efficiency. For example, the filter membrane or silk cloth used for filtration needs to be sterilized and bandaged, and the process is cumbersome and requires high operation. ;Affected by operating techniques and other factors, there may be liquid leakage, resulting in an unguaranteed clarification effect; the clarification effect may be affected by the difference in batches of filter membranes or silk cloth, and the pore size of different batches of silk cloth varies from batch to batch. For multiple uses, the integrity needs to be verified each time; it is not easy to carry out large-scale clarification, and the use of filter membranes and silk cloth has higher requirements for a single filtration volume. When the single volume is too large, the quality of the first filtration of the same batch is lower Good, the subsequent accumulation of impurities will cause blockage of the filter membrane or silk cloth, affecting the local filtration effect, which may cause excessive local pressure and destroy the structure of the virus

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