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Method for extracting bio-polypeptides

An extraction method and biological polypeptide technology, which are applied in the field of immune promotion, can solve the problems of high energy consumption, time-consuming and high production cost, and achieve the effects of increasing yield, improving economic benefits and low cost.

Inactive Publication Date: 2017-02-22
广东美利奥生物科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The freeze-thaw method requires repeated freezing during the production process, which is a relatively time-consuming and energy-consuming link, and the optimal number of freeze-thaw times needs to be determined, so the production cost is relatively high

Method used

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Embodiment Construction

[0024] In order to allow those skilled in the art to understand the present invention more clearly and intuitively, the present invention will be further described.

[0025] A method for extracting biological polypeptides, comprising the steps of:

[0026] (1) After cutting off the fat and outer envelope of the spleen of fresh healthy animals, grind it with a meat grinder and weigh it. The ratio of material to liquid is 1:10. Homogenize the spleen with a high-speed homogenizer at 15000-25000rpm in an ice bath. Slurry for 15-30 minutes, coarsely filter with 8 layers of gauze, centrifuge at 12000×g, 4°C for 20 minutes in a continuous flow centrifuge, collect the supernatant, so that the particle size of the obtained polypeptide is about 0.5 μm-50 μm;

[0027] (2) Treat the obtained supernatant with a 500-2500W microwave extractor for 15-45 minutes, and then quickly place it in ice water at 4°C, so that the particle size of the obtained polypeptide is about 0.05 μm-10 μm;

[002...

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Abstract

The invention provides a method for extracting bio-polypeptides. The method comprises the following steps: removing fat and external envelops from brains, livers, spleens or thymus glands of fresh seafoods (oyster, shrimp flesh, crab flesh, sea cucumber and the like) and healthy animals, and grinding with a meat grinder; weighing, and homogenizing at high speed; treating the homogenized liquid in microwave for 15-30 minutes by adopting a continuous flow centrifuge centrifuging technology / gauze coarse filtering treatment, quickly standing in ice water at 4 DEG C, and performing ultrasonic treatment for 30-60 minutes; and performing ultrafiltration by utilizing a lateral ultrafiltration membrane having a molecular weight cut-off of 1000-12500 dlotons, and freezing and drying the ultrafiltration liquid to obtain the bio-polypeptide dry powder. The method can be used for increasing the bio-polypeptide yield to 230-450 percent, so that the yield of extracting bio-polypeptides from animal organs can be improved.

Description

technical field [0001] The invention relates to the technical field of immune promotion, in particular to a method for extracting biological polypeptides. Background technique [0002] In today's society, science and technology are developing rapidly, so people's living standards are gradually improving, and the quality of life is getting better. But at the same time, fierce industry competition and faster and faster pace of life make human beings face more and more pressure in life and work, and pressure and disease are closely linked. Studies have shown that people's stress is closely related to the immune system. When people are in a state of stress for a long time, their immune function will decline, and the body's sensitivity to diseases will increase, which will easily lead to susceptibility and susceptibility to diseases. There are also reports showing that smoking is harmful to the human body in many ways, one of which is to damage the immune function of the human ...

Claims

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Application Information

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IPC IPC(8): C07K1/34C07K1/14
CPCC07K1/14C07K1/34
Inventor 臧林泉邓晓阳
Owner 广东美利奥生物科技有限公司
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