Method for industrially producing new born calf purified serum

A technology of newborn cattle and blood serum, applied in the biological field, to achieve the effect of easy implementation of environmental protection measures

Inactive Publication Date: 2019-07-30
范清春
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This technology fills the gap in the world and realizes industrial production; the serum purification tec...

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Examples

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Embodiment Construction

[0014] In order to better understand the present invention, the method is described in detail by taking 1000L raw serum as an example. The methods described in the following examples are conventional methods unless otherwise specified, and the reagents and materials are all commercially available unless otherwise specified.

[0015] The specific implementation method is as follows.

[0016] (1) Serum thawing: Newborn bovine serum fed with colostrum that contains lipids, endotoxins, and hemoglobin exceeds the standard. After collection, the raw materials are frozen at -10°C, thawed at 4-10°C, and combined after thawing.

[0017] (2) Pre-filtration: Use a 25 μm polypropylene cloth bag filter to filter particles such as fibrin (original) to protect the ultrafiltration column. Usually, a No. 2 cloth bag can successfully filter 1000L of serum.

[0018] (3) Ultrafiltration: Fully dialysis with an ultrafiltration column with a molecular weight cut-off of 100-300kd from Spectrum Com...

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Abstract

The invention relates to a method for animal serum purification. New born calf raw material serum which is obtained after beestings is fed and contains a large amount of lipid, endotoxin, hemoglobin and other impurities is preliminarily filtered to remove fibrous protein; the serum is separated into an interception part (a macromolecule compound) and a dialysis part (free molecules) through an ultrafiltration column with the molecular weight cutoff being 100-300 kd; a solid phase deproteinization hydration shell medium is added into the interception part so as to promote lipid (containing chyle particles and lipoprotein), immune globulin, endotoxin, hemoglobin and other impurities to be polymerized into flocks, and continuous flow centrifuging and clarification filtering are performed to obtain clear liquid; the clear liquid and the ultrafiltration dialysis part are combined, and after degerming, new born calf purified serum is manufactured. By adopting the method, the new born calf serum which is obtained after beestings is fed and contains excessive endotoxin and hemoglobin can be processed into serum meeting the quality requirement of a cell culture medium for vaccines and an ELISA kit.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for industrialized production of purified serum of newborn cattle. Background technique [0002] Neonatal bovine serum is the most important cell culture medium for the production of human vaccines and veterinary vaccines. It is also an important auxiliary material for enzyme immunoassay kits, and the market demand is huge. In my country, newborn bovine serum is the serum made by aseptically collecting the arterial blood of newborn bulls that have not eaten. The Pharmacopoeia requires that the endotoxin is less than 10EU / ml, the hemoglobin is less than 200mg / mL, and the appearance is clear. Newborn bovine serum from New Zealand, Australia and other sources. Newborn bulls have been fed colostrum for 4-14 days, and the serum made by neck bloodletting contains a lot of lipid components, and the appearance is turbid and viscous, which is difficult to sterilize and filter; usuall...

Claims

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Application Information

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IPC IPC(8): C12N5/071G01N33/53
CPCC12N5/0602G01N33/53
Inventor 范清春
Owner 范清春
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