Method for industrially producing new born calf purified serum

Abstract

The invention relates to a method for animal serum purification. New born calf raw material serum which is obtained after beestings is fed and contains a large amount of lipid, endotoxin, hemoglobin and other impurities is preliminarily filtered to remove fibrous protein; the serum is separated into an interception part (a macromolecule compound) and a dialysis part (free molecules) through an ultrafiltration column with the molecular weight cutoff being 100-300 kd; a solid phase deproteinization hydration shell medium is added into the interception part so as to promote lipid (containing chyle particles and lipoprotein), immune globulin, endotoxin, hemoglobin and other impurities to be polymerized into flocks, and continuous flow centrifuging and clarification filtering are performed to obtain clear liquid; the clear liquid and the ultrafiltration dialysis part are combined, and after degerming, new born calf purified serum is manufactured. By adopting the method, the new born calf serum which is obtained after beestings is fed and contains excessive endotoxin and hemoglobin can be processed into serum meeting the quality requirement of a cell culture medium for vaccines and an ELISA kit.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for industrialized production of purified serum of newborn cattle. Background technique [0002] Neonatal bovine serum is the most important cell culture medium for the production of human vaccines and veterinary vaccines. It is also an important auxiliary material for enzyme immunoassay kits, and the market demand is huge. In my country, newborn bovine serum is the serum made by aseptically collecting the arterial blood of newborn bulls that have not eaten. The Pharmacopoeia requires that the endotoxin is less than 10EU / ml, the hemoglobin is less than 200mg / mL, and the appearance is clear. Newborn bovine serum from New Zealand, Australia and other sources. Newborn bulls have been fed colostrum for 4-14 days, and the serum made by neck bloodletting contains a lot of lipid components, and the appearance is turbid and viscous, which is difficult to sterilize and filter; usuall...

AI Technical Summary

Problems solved by technology

This technology fills the gap in the world and realizes industrial production; the serum purification technology to remove impurities such as lipids, endotoxins and hemoglobin has not been reported at home and abroad