Serum-free medium suitable for large-scale single-cell suspension culture of young hamster kidney cells

A technology of serum-free medium and hamster kidney cells, which is applied in the field of cell culture medium and can solve the problems of unpatented and unpublished sales
CN102268403AInactive Publication Date: 2011-12-07上海米迪生物技术有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
上海米迪生物技术有限公司
Publication Date
2011-12-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a serum-free medium suitable for large-scale single-cell suspension culture of young hamster kidney cells (BHK cells), including 21 kinds of amino acids, 11 kinds of inorganic salts, 12 kinds of vitamins, 1 kind of protein hydrolyzate, 2 kinds of Lipids, 2 buffer components and 6 additives. The BHK cell serum-free medium of the present invention produces biological products by single-cell suspension culture, which can not only avoid various disadvantages of serum culture, but also eliminate the problem of difficult scale-up of roller bottle adherent culture, thereby improving the production efficiency of BHK cell culture And reduce production costs, while ensuring product quality, has good application value and huge market prospects.
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Description

technical field

[0001] The invention relates to the field of cell culture medium, in particular to a serum-free culture medium suitable for large-scale single-cell suspension culture of young hamster kidney cells. Background technique

[0002] Rabies is a natural foci or zoonotic acute infectious disease caused by rabies virus. It is widespread and has a high fatality rate. Strict management of dogs, dog immunization or extermination of dogs is a positive way to control human rabies. Useful ways. At present, there is no specific and effective treatment for the disease, and the fatality rate is almost 100%. Foot-and-mouth disease is an acute, febrile, highly contagious infectious disease of cloven-hoofed animals. Its virus mutation and epidemic spread are extremely fast, and it can infect more than 70 kinds of domestic and wild cloven-hoofed animals. It has a worldwide impact on animal husbandry and related industries. The industry has had a considerable impact. The World Or...

Claims

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