Serum-free medium suitable for large-scale single-cell suspension culture of young hamster kidney cells

A technology of serum-free medium and hamster kidney cells, which is applied in the field of cell culture medium and can solve the problems of unpatented and unpublished sales

Inactive Publication Date: 2011-12-07
上海米迪生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The MD611 medium produced by Tsingda Tianyi, a leading medium company in China, can be effectively used as a low-serum medium for the adherent culture of BHK

Method used

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  • Serum-free medium suitable for large-scale single-cell suspension culture of young hamster kidney cells
  • Serum-free medium suitable for large-scale single-cell suspension culture of young hamster kidney cells
  • Serum-free medium suitable for large-scale single-cell suspension culture of young hamster kidney cells

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Experimental program
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Effect test

Embodiment 1

[0143] A serum-free medium suitable for large-scale single-cell suspension culture of young hamster kidney cells in this embodiment, which includes 21 kinds of amino acids, 11 kinds of inorganic salts, 12 kinds of vitamins, 1 kind of protein hydrolyzate, 2 kinds of lipids, 2 buffer components, 6 additives; materials described below were purchased from Sigma.

[0144] A specific embodiment of the serum-free medium of this example, labeled BS-SFM, was prepared using the ingredients and amounts listed in Table 1.

[0145] Table 1:

[0146] Element concentration calcium chloride 116mg / L 50 x major salts 20ml / L 10000×minor salts 0.1ml / L 200 x vitamins and other substances 5ml / L glucose 4500mg / L Glutamine 484mg / L 10 x Amino Acids 100ml / L 10% Pluronic F-68 10ml / L sodium bicarbonate 2600mg / L rice hydrolyzate 2000mg / L

[0147] Table 2: 50 x Major Salts

[0148] Element Concentration (mg / ...

Embodiment 2

[0161] A serum-free medium suitable for large-scale single-cell suspension culture of baby hamster kidney cells in this embodiment, which includes 21 kinds of amino acids, 11 kinds of inorganic salts, 12 kinds of vitamins, 1 kind of protein hydrolyzate, 2 kinds of lipids, 2 buffer components, 6 additives; materials described below were purchased from Sigma.

[0162] The ingredients are as follows, expressed in mg / l:

[0163] L-Tryptophan 350

[0164] L-cysteine ​​150

[0165] L-Isoleucine 200

[0166] L-Leucine 300

[0167] L-Valine 220

[0168] L-arginine hydrochloride 258

[0169] L-Histidine Hydrochloride 500

[0170] L-Lysine 300

[0171] L-Methionine 50

[0172] L-glutamic acid 25

[0173] L-phenylalanine 25

[0174] L-Asparagine 420

[0175] Glycine 20

[0176] L-proline 19

[0177] L-alanine 20

[0178] L-Aspartic Acid 20

[0179] L-Serine 300

[0180] L-threonine 50

[0181] L-Glutamine 390

[0182] L-cystine hydrochloride 45

[0183] L-Tyrosine diso...

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Abstract

The invention discloses a serum-free medium suitable for large-scale single-cell suspension culture of young hamster kidney cells (BHK cells), including 21 kinds of amino acids, 11 kinds of inorganic salts, 12 kinds of vitamins, 1 kind of protein hydrolyzate, 2 kinds of Lipids, 2 buffer components and 6 additives. The BHK cell serum-free medium of the present invention produces biological products by single-cell suspension culture, which can not only avoid various disadvantages of serum culture, but also eliminate the problem of difficult scale-up of roller bottle adherent culture, thereby improving the production efficiency of BHK cell culture And reduce production costs, while ensuring product quality, has good application value and huge market prospects.

Description

technical field [0001] The invention relates to the field of cell culture medium, in particular to a serum-free culture medium suitable for large-scale single-cell suspension culture of young hamster kidney cells. Background technique [0002] Rabies is a natural foci or zoonotic acute infectious disease caused by rabies virus. It is widespread and has a high fatality rate. Strict management of dogs, dog immunization or extermination of dogs is a positive way to control human rabies. Useful ways. At present, there is no specific and effective treatment for the disease, and the fatality rate is almost 100%. Foot-and-mouth disease is an acute, febrile, highly contagious infectious disease of cloven-hoofed animals. Its virus mutation and epidemic spread are extremely fast, and it can infect more than 70 kinds of domestic and wild cloven-hoofed animals. It has a worldwide impact on animal husbandry and related industries. The industry has had a considerable impact. The World Or...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12N5/02
Inventor 张大鹤滕小锘龚迪
Owner 上海米迪生物技术有限公司
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