30 results about "Biogenic peptide" patented technology

The invention relates to the field of preparation and application of biological peptides, in particular to a swelling hippocampal peptide and application thereof in preparation of uric acid reducing and gout resisting products. The amino acid sequence is shown as SEQ ID NO.1. The uric acid reducing effect of the compound is proved through in-vitro xanthine oxidase (XOD) and adenosine deaminase (ADA) inhibition experiments and cell experiments; on the basis, the uric acid reducing and gout resisting activity of the peptide sequence is further verified by means of a hyperuricemia animal model. The achievement provides a solid theoretical support for high-value development and utilization of the hippocampus japonicus and research and development of uric acid reducing and gout resisting related products.

The invention discloses a corn peptide for dispelling effects of alcohol and protecting liver as well as a preparation method and application thereof, and belongs to the technical field of biological peptide extraction. The preparation method comprises the following steps: crushing and sieving corn, dissolving the corn in deionized water, adjusting the pH value to be alkaline, and performing ultrasonic-assisted extraction to obtain corn homogenate; centrifuging, taking supernate, adjusting the pH to be neutral, centrifuging, adding an ethanol solution with the mass fraction of 10%, standing, carrying out continuous flow centrifugation, suspending precipitates in distilled water, dialyzing in a dialysis bag, and freeze-drying to obtain crude corn protein; the preparation method comprises the following steps: preparing an aqueous solution, carrying out variable-temperature treatment, adjusting the pH value to be alkaline, adding trypsin and alkaline protease, carrying out enzymolysis, repeating the enzymolysis twice, and after the enzymolysis is finished, carrying out heating enzyme deactivation, centrifugation, sediment discarding, ultrafiltration, centrifugation, supernatant taking and spray drying to obtain the corn peptide. The corn peptide can remarkably shorten the drunkenness time and sobering time of mice and reduce the drunkenness duration, and has good effects of dispelling the effects of alcohol and protecting the liver.

The present application relates to the preparation and application field of biological peptide, and more particularly to a hippocampus abdominalis peptide and its application in preparation of products for reducing uric acid and resisting gout. The amino acid sequence is shown as SEQ ID NO. 1, and the present application confirms the uric acid reducing effect through in vitro xanthine oxidase (XOD) and adenosine deaminase (ADA) inhibition experiments and cell experiments; on this basis, the uric acid reducing and gout resisting activity of the peptide sequence is further verified by means of a high uric acid animal model. This achievement provides a solid theoretical support for high-value development and utilization of hippocampus abdominalis, and research and development of products for reducing uric acid and resisting gout.

This invention belongs to the field of biopeptide extraction technology, specifically relating to a water lily polypeptide, its extraction method, and its application. The invention involves sequentially disinfecting, washing, drying, and pulverizing the water lily sample to remove impurities from its surface. Heating the pulverized sample with water facilitates the initial precipitation of active ingredients from the water lily. Adding papain for enzymatic hydrolysis and then using an ethanol solution for analysis helps to penetrate the water lily sample, fully releasing the internal water lily polypeptides while maintaining high activity. Therefore, the water lily polypeptide provided by this invention has high activity and can be used in anti-wrinkle and whitening products.

The invention discloses an intestine-moistening and stomach-protecting wheat peptide and a preparation method and application thereof, belongs to the technical field of biological peptides, and particularly relates to a preparation method of the intestine-moistening and stomach-protecting wheat peptide, which comprises the following steps: pre-mixing wheat gluten protein with water to obtain a wheat gluten protein aqueous solution; dropwise adding a NaOH aqueous solution, centrifuging, and taking supernate; adding citric acid to adjust the pH value, standing, and taking a precipitate; dissolving the precipitate in water to obtain a protein solution, adding a compound enzyme preparation, carrying out enzymolysis to obtain a peptide solution, centrifuging, taking a supernatant to obtain a wheat peptide solution, adding mannitol, then adding glycine, and carrying out ultrafiltration and freeze drying to obtain wheat peptide; the compound enzyme preparation comprises ProteAX enzyme, Alcalase protease and EDTA (Ethylene Diamine Tetraacetic Acid). By adjusting the preparation method and matching with the special compound enzyme preparation prepared from ProteAX enzyme, Alcala enzyme protease and EDTA, the intestine moistening and stomach protecting effects of the prepared wheat peptide are improved.

The invention belongs to the technical field of biological peptides and application, and particularly relates to an antioxidant peptide with a Keap1 inhibiting effect and application of the antioxidant peptide. The antioxidant peptide provided by the invention comprises antioxidant pentapeptide, antioxidant tripeptide and the like which all show good in-vitro antioxidant activity, the scavenging rate of ABTS free radicals reaches 0.69-1.11 [mu] mol TE / [mu] mol, the scavenging rate of DPPH free radicals reaches 30.11%-34.94%, in addition, the antioxidant peptide can activate the expression of Nrf2 and downstream genes HO-1 and NQO1 thereof by inhibiting the expression level of Keap1 protein, so that the antioxidant activity of the antioxidant peptide is improved. Therefore, the compound has a wide application prospect in the fields of cosmetics, medicines, foods and the like.

The invention relates to the technical field of high-value utilization of marine biological resources, and discloses a high-activity marine biological peptide prepared by an enzymolysis method and application of the high-activity marine biological peptide in food. The target high-activity marine biological peptide and an affinity molecular switch sensitive to the concentration change of the target high-activity marine biological peptide are jointly fixed on a magnetic nano-carrier with bitter peptide adsorption functional groups on the surface to obtain the intelligent enzyme carrier; and adding the intelligent enzyme carrier into a reaction system containing a marine protein raw material and a first amphiphilic microcapsule wall material to carry out an enzymatic hydrolysis reaction in the reaction process. By constructing an intelligent enzyme carrier integrated with a directional enzyme and an affinity molecular switch, precise cutting and process self-control of an enzymolysis reaction are realized, and the affinity molecular switch can respond to the change of the concentration of the high-activity marine biological peptide and spontaneously adjust the enzyme activity, so that excessive degradation of a target product is effectively avoided.

This invention discloses an abalone-derived xanthine oxidase inhibitory peptide and its application, belonging to the field of biopeptides. Using abalone as raw material, this invention employs a mild blanching-ultra-high pressure-ultrasound synergistic pretreatment process, followed by enzymatic hydrolysis, separation and purification, and bioinformatics screening combined with molecular docking technology to obtain four polypeptides with amino acid sequences as shown in SEQ ID NO. 1-4. Experimental results show that the abalone-derived polypeptides provided by this invention have outstanding XOD inhibitory activity, with a half-maximal inhibitory concentration as low as 0.53 mg / mL, superior to existing shellfish peptides. They are non-toxic or non-sensitizing and can effectively reduce uric acid and pro-inflammatory factor levels in a hyperuricemia cell model, and can be applied to the preparation of uric acid-lowering drugs. This invention effectively overcomes the shortcomings of low activity and limited sources of natural peptides, promotes the high-quality utilization of abalone protein resources, improves the level of deep processing in the abalone industry, and provides novel natural candidate substances and technical solutions for the prevention and treatment of hyperuricemia.

The invention belongs to the technical field of biological peptide processing, and particularly discloses oyster peptide for improving male functions and a preparation method of the oyster peptide. The preparation method comprises the following steps: S1, mixing fresh oyster meat with an algae substance, adding deionized water, grinding, adjusting the pH value to 6.5-7.0, and carrying out ultrasonic treatment to obtain a homogenate; s2, adding compound protease into the homogenate obtained in the step S1, and performing enzymolysis to obtain enzymatic hydrolysate; s3, adding an organic matter into the enzymatic hydrolysate obtained in S2, and heating for reaction to obtain a reaction solution; s4, adjusting the pH value of the reactant obtained in S3 to 5.5-6.5, adding lactic acid bacteria, and fermenting to obtain fermentation liquor; and S5, performing inactivation and ultrafiltration on the fermentation liquor obtained in the step S4 to obtain the oyster peptide. The invention discloses an oyster peptide capable of improving male functions and a preparation method thereof. The oyster peptide obtained by adopting the preparation method is high in yield, free of fishy smell and capable of remarkably enhancing male function activity.

The invention belongs to the technical field of biological peptide enzymolysis, and particularly discloses a sheep three-medullary peptide composition for assisting in improving memory and application of the sheep three-medullary peptide composition. The sheep three-medullary peptide composition is prepared from the following components in parts by mass: 40 to 50 parts of sheep three-medullary peptide, 20 to 35 parts of sheep colostrum peptide and 10 to 25 parts of casein hydrolyzed peptide. The invention discloses a sheep three-medullary peptide composition for assisting in improving memory and application thereof, which can obviously improve the level of acetylcholine in the brain and inhibit the activity of acetylcholin esterase to enhance nerve conduction, and can effectively relieve the damage of oxidative stress to brain tissues by improving the activity of SOD and GSH-Px and reducing the content of MDA.

The invention provides an antioxidant biological peptide as well as a preparation method and application thereof. The modified sodium alginate is added into the antioxidant skin-care biological peptide composition, and the antioxidant biological peptide is combined into a double-crosslinking structure of the modified sodium alginate, so that better antioxidant effect and stability can be realized. The modified sodium alginate is formed through multi-step treatment, so that the problems that the traditional biological peptide is unstable in structure and is easily influenced by the environment to lose efficacy can be solved, and the action time of the biological peptide can be prolonged; the gallic acid has oxidation resistance and can be in synergistic interaction with the biological peptide, so that a foundation is laid for high-efficiency oxidation resistance of the composition. In the composition, the modified sodium alginate can improve the compatibility with other components and the system stability, and enhance the overall free radical scavenging ability; meanwhile, biological peptides are protected, activity attenuation in storage is reduced, the anti-degradation capacity of the composition is enhanced, and the problems that existing antioxidant cosmetics are poor in stability and poor in long-term use effect are solved.

The present application relates to the preparation and application field of biological peptide, and particularly relates to a plectinum peptide and application of the plectinum peptide in preparation of a product for repairing male reproductive system damage function. The present application utilizes green controllable enzymolysis technology to obtain protein polypeptides with nutritional health care and medicinal values, and further researches on the basis to obtain an active peptide sequence with high biological activity, obtains the active peptide with high purity through a chemical synthesis mode, and verifies the biological activity of the peptide by using mouse spermatogonia.

The invention relates to the technical field of agricultural biological agents and foliage dressing, in particular to a novel fertilizer which comprises the following components in percentage by mass: 30% of microalgae biogenic peptide, 10% of yeast sugar, 5% of an adsorbent, 1% of an activating agent, 1% of a colloidal agent, 2% of a water absorbent, 1% of a complexing agent and 50% of purified water. In an early rice test, the yield is increased by 19.3%-29.1% compared with that of CK after the photosynthetic carbon fertilizer is sprayed, the yield is superior to that of a conventional photosynthetic carbon fertilizer, the CO2 concentration around crops is increased to 580-1000 ppm, photosynthesis is enhanced in the daytime, light breathing is inhibited at night, more carbohydrates are accumulated, chemical fertilizer application can be reduced, the pesticide residue and heavy metal content can be reduced, the quality of agricultural products is improved, pest reproduction is inhibited through the high CO2 concentration, the stress resistance of the crops is enhanced, and the yield of the crops is increased. And the fertilizer can be used for planting, livestock raising and aquatic products, so that multiple purposes of one fertilizer are realized.

The application relates to the technical field of bioinformatics, and discloses a bioactive peptide activity data analysis method and system, which comprises the following steps: constructing an embedding vector for an input bioactive peptide sample; based on the embedding representation, combining a preset candidate model set, and calculating the fitting probability between each model and the sample; constructing a path graph structure with the model as a node, introducing a model switching cost function, and jointly optimizing the inference path based on the fitting probability and the cost; executing the inference process of the model in the path, and completing the sequential transmission and scheduling control of the intermediate results; and finally integrating all the model outputs to generate a bioactive peptide activity prediction result. The system supports dynamic path planning, inference process fault tolerance and execution optimization, and improves the accuracy and efficiency of bioactive peptide analysis. The application effectively solves the problems of single model fitting, low resource utilization and unstable execution process in the prior art, and has good universality and expansion capability.

The invention belongs to the technical field of biological peptide extraction, and particularly discloses cattle spleen peptide for improving immunity and a preparation method and application thereof. Comprising the following steps: adding cattle spleen into ice water, grinding, carrying out microwave-assisted extraction, centrifuging, and taking supernatant; adding an n-hexane-absolute ethyl alcohol mixed solution, centrifuging, taking a supernatant, adding alkaline protease, performing enzymolysis, after enzymolysis is finished, heating for enzyme deactivation, centrifuging, taking a supernatant, and performing freeze-drying, so as to obtain cattle spleen crude protein; mixing the cattle spleen with deionized water, performing autoclaved sterilization, inoculating saccharomycetes and bacillus subtilis, and performing fermentation to obtain cattle spleen fermentation liquor; and heating in a water bath, centrifuging, discarding the precipitate, dialyzing in a dialysis bag, adding trypsin for enzymolysis, after enzymolysis is finished, heating for enzyme deactivation, centrifuging, discarding the precipitate, carrying out ultrafiltration, centrifuging, taking the supernatant, and carrying out spray drying to obtain the cattle spleen peptide. The bovine spleen peptide can specifically act on macrophages and lymphocytes, so that the phagocytic activity of the macrophages is remarkably enhanced, and the immunity of the organism is improved.

This invention relates to the technical field of extraction devices, and discloses a biopeptide extraction device for biochemical detection. The device includes an extraction tank, a stirring shaft rotatably connected inside the extraction tank, and a guide ring fixedly connected inside the extraction tank. The number of segmented rings is half the number of outer rings, allowing a slider to move horizontally reciprocating a distance at different times. Simultaneously, the slider causes the segmented rings to move vertically reciprocating synchronously. The slider drives the outer blades to perform differentiated horizontal reciprocating motions. Utilizing the diameter differences in different sections of the guide ring, the outer blades create a wide-range sweeping motion in the radial direction, covering areas that are difficult for traditional stirring blades to reach, such as the inner wall of the extraction tank. This effectively prevents the solid biomaterial from settling and accumulating at the radial edges, and the segmented blades precisely disturb the material in each area, eliminating axial stirring blind spots in the upper and lower areas of the extraction tank. The combined stirring action of these two actions constructs a composite flow field, ensuring that the solid biomaterial in all areas of the tank can fully contact the extraction solvent.

The invention belongs to the technical field of biological medicines, and particularly relates to an antibacterial peptide GT-2 and application thereof. The amino acid sequence of the antibacterial peptide GT-2 is as shown in SEQ ID NO: 2. The antibacterial peptide GT-2 provided by the invention is obtained by modifying a natural antibacterial peptide GP-1 extracted from gastrodia elata, the antibacterial peptide GT-2 has the effects of broad-spectrum bacteriostasis, high antibacterial efficiency, low toxicity and high safety, and an effective way is provided for providing a bacteriostatic biological peptide with practicability, high efficiency and safety.

The invention relates to the field of preparation and application of biological peptides, in particular to a salvia miltiorrhiza SmIDL1 polypeptide as well as a preparation method and application thereof. The amino acid sequence of the polypeptide is as shown in SEQ ID NO. 1; according to the method disclosed by the invention, by simulating an endogenous senescence / nutrition re-mobilization signal, the directional transport of a photosynthetic product to the root and the preferential distribution of the photosynthetic product to tanshinone biosynthesis are accurately regulated and controlled, so that the specific efficient accumulation of tanshinone components is realized on the premise of not remarkably promoting vegetative growth. The invention provides a brand new precise agricultural strategy of material flow regulation and quality improvement, and has important value for producing high-quality salvia miltiorrhiza medicinal materials with high tanshinone content.

The invention relates to the field of preparation and application of biological peptides, in particular to a swelling hippocampal peptide and application of the swelling hippocampal peptide in preparation of anti-fatigue products. An active peptide sequence with high biological activity is obtained through research, animal behavioral experiments prove that the belly-expanding hippocampal peptide can prolong the swimming exhaustion time and enhance the muscle strength and has a remarkable anti-fatigue effect, and a new way is provided for development of hippocampal anti-fatigue products.

The invention belongs to the technical field of biological peptide extraction, and particularly discloses blood protein polypeptide as well as a preparation method and application thereof. Comprising the following steps: S1, collecting fresh animal blood, adding a sodium citrate solution, centrifuging, discarding plasma, and collecting blood cells; s2, adding the blood cells obtained in the step S1 into purified water, performing uniform mixing, adjusting the pH value to be acidic, adding papain, performing first enzymolysis, after enzymolysis is finished, performing heating for enzyme deactivation, performing centrifugation, and taking supernate, so as to obtain enzymatic hydrolysate; and S3, adjusting the pH value of the enzymatic hydrolysate obtained in S2 to be alkaline, adding alkaline protease, carrying out secondary enzymolysis, and after the enzymolysis is finished, carrying out heating enzyme deactivation, centrifugation, sediment discarding, ultrafiltration, centrifugation, supernatant taking and freeze-drying to obtain the blood protein polypeptide. The invention discloses a blood protein polypeptide as well as a preparation method and application thereof. The blood protein polypeptide is applied to food or health care products and can effectively improve anemia and recover the number of red blood cells.

Disclosed is use of starch for providing benefit selecting from the group consisting of inducing secretion of anti-microbial peptide, protecting from microbial infection, improving barrier function boosting innate defense, upregulating the gene expression of cathelicidin antimicrobial peptide or the gene expression of human beta-defensin-4.

The invention relates to the field of preparation and application of biological peptides, in particular to a salvia miltiorrhiza SmCLE45-3 polypeptide as well as a preparation method and application thereof. The amino acid sequence of the polypeptide is as shown in SEQ ID NO. 1; by utilizing the system regulation function of the plant endogenous signal peptide, the salvia miltiorrhiza terpene and phenylpropane metabolic pathway can be efficiently and synergistically activated, and the salvia miltiorrhiza root biomass and the content and accumulation of tanshinone IIA, cryptotanshinone and salvianolic acid B are synchronously and remarkably improved on the premise of not inhibiting plant growth. The technology has the advantages of being accurate in effect, safe, environmentally friendly, low in dosage, easy and convenient to operate and the like, and is suitable for high-quality and high-yield standardized cultivation of salvia miltiorrhiza.

The present application provides an immune inducer comprising a polynucleotide-peptide conjugate or a pharmaceutically acceptable salt thereof as an effective ingredient, the polynucleotide-peptide conjugate consisting of a single-stranded polynucleotide or polynucleotide derivative containing a CpG motif, a peptide, and a spacer covalently bonded at one end to the polynucleotide or polynucleotide derivative and covalently bonded at the other end to the peptide, the peptide being a peptide in which one or more consecutive amino acids at the N-terminus of an MHC-binding peptide are replaced with an amino acid having a reactive functional group for forming a covalent bond with the spacer, wherein the one or more consecutive amino acids do not include anchor residues for binding to MHC.

The application belongs to the technical field of biological medicine, and particularly relates to an antibacterial peptide GT-2 and application thereof. The amino acid sequence of the antibacterial peptide GT-2 is shown as SEQ ID NO:2. The antibacterial peptide GT-2 provided by the application is obtained by modifying a natural antibacterial peptide GP-1 extracted from Gastrodia elata Blume. The antibacterial peptide GT-2 has the effects of broad-spectrum bacteriostasis, high antibacterial efficiency, low toxicity and high safety. The application provides an effective way for providing a bacteriostatic biological peptide with practicability, high efficiency and safety.

The invention belongs to the technical field of biological peptide evaluation, and discloses a machine learning-based beta-lactoglobulin ACE inhibitory peptide multi-attribute evaluation method. The method comprises the following steps: 1, collecting related data of the beta-lactoglobulin ACE inhibitory peptide, and constructing a multi-attribute evaluation model; step 2, acquiring related data of the target beta-lactoglobulin ACE inhibitory peptide, and outputting an evaluation value of each attribute; 3, obtaining the contribution degree of each attribute according to the demand scene, and correcting the evaluation value of each attribute based on the contribution degree; 4, obtaining a comprehensive evaluation value according to the influence coefficient; 5, performing grading evaluation based on the comprehensive evaluation value; step 6, adjusting and optimizing a grading evaluation result; according to the method, the influence coefficients of different attributes can be dynamically generated according to the mutual influence condition of different attributes in different scenes, so that the evaluation value is corrected, and the evaluation of the beta-lactoglobulin ACE inhibitory peptide is more accurate.

The invention discloses a microalgae polypeptide and application thereof in preparation of a product for preventing and / or treating hyperuricemia, and belongs to the field of preparation and application of biological peptides. The microalgae polypeptide obtained by the invention comprises at least one of polypeptide 1, polypeptide 2 and polypeptide 3. The amino acid sequence of the polypeptide 1 is as shown in SEQ ID NO. 1; the amino acid sequence of the polypeptide 2 is LAW; the amino acid sequence of the polypeptide 3 is as shown in SEQ ID NO. 2. The prepared microalgae polypeptide component has good XOD inhibitory activity, and can be applied to preparation of uric acid reducing products and xanthine oxidase inhibitors. According to the invention, the utilization rate and industrial value of microalgae protein resources can be effectively improved, and meanwhile, a new technical idea and a new method are provided for prevention and treatment of hyperuricemia.

The invention belongs to the technical field of biological peptides, and particularly relates to an application of a polypeptide in preparation of an anti-aging skin care product. The invention provides application of polypeptide in preparation of an anti-aging skin care product. The amino acid sequence of the polypeptide is as follows: Ala-Glu-Pro-Val-Pro-Leu. The polypeptide provided by the invention can significantly reduce the length and depth of the forehead wrinkles of a subject; the area and depth of the Chinese character'chuan '-shaped patterns are obviously reduced; the number and depth of crow's feet are obviously reduced; the volume and the depth of nasolabial folds are obviously reduced, so that the polypeptide disclosed by the invention has an obvious anti-wrinkle effect. The polypeptide essence disclosed by the invention is mild and non-irritant, and a subject with sensitive skin does not have untoward effects related to the polypeptide disclosed by the invention.

The application discloses a marine organism peptide capable of effectively promoting skin wound repair, and a preparation method and application thereof. The marine organism peptide has good skin compatibility, can significantly promote cell proliferation, migration and vascular formation, effectively reduces inflammatory reaction, promotes high-quality repair of wounds, and reduces scar formation. The preparation method of the marine organism peptide comprises the following steps: taking a fresh and live soft body of a marine shellfish, performing protease enzymolysis, ultrafiltration and dextran gel separation, discarding an elution single peak in the last step, collecting or mixing other elution components, and freeze-drying to obtain the marine organism peptide. The method is simple, low in cost, green and environment-friendly, short in production period, and suitable for industrial production. The marine organism peptide can be applied to medicines, medical biomaterials and cosmetics, has a wide application range, and has a remarkable effect on promoting regeneration and repair of burn, scald, cutting, traffic, surgical, war injury, firearm injury combined with seawater immersion injury, diabetic ulcer, inflammatory ulcer, pressure ulcer and chronic refractory wounds.

This invention discloses a calcium and iron-enhancing donkey-hide gelatin peptide prepared based on a gastrointestinal digestion model, its preparation method, and its applications, belonging to the field of biotechnology. By comparing different animal gelatin bases, this invention selects highly active donkey-hide gelatin as a raw material and simulates in vitro gastrointestinal dynamic digestion to prepare highly absorbable calcium and iron active peptides, effectively improving peptide bioavailability. The preparation method described in this invention can efficiently screen for calcium and iron-enhancing and highly permeable bioactive peptides. The method is simple and easy to implement, enabling high-throughput screening of hundreds of candidate peptides or formulations. The screened peptide products can be specifically applied in functional foods and pharmaceuticals, showing significant market potential and providing new ideas for related research.

The invention relates to absorbable bone wax for promoting bone healing and a preparation method thereof, and belongs to the technical field of bone healing materials, the bone wax comprises a degradable copolymer, PTMC, magnesium-calcium silicate-biological peptide composite microspheres, mesoporous silica loaded panax notoginseng saponins and hydrogenated castor oil. The degradable copolymer is prepared by reacting PEG (Polyethylene Glycol), stannous octoate, D, L-lactide and epsilon-caprolactone and precipitating. The magnesium-calcium silicate-biological peptide composite microspheres are prepared by loading biological peptide on porous magnesium-calcium silicate microspheres and then coating the biological peptide with polydopamine. The biological peptide is a peptide fragment between 1kDa and 5kDa obtained by carrying out step-by-step enzymolysis on urechis unicinctus through papain and trypsin. All the components are compounded according to a specific proportion to prepare the bone wax, the limitation that traditional bone wax is poor in degradability and single in function is broken through, and the integrated functions of hemostasis, degradation and efficient bone induction are achieved. Through cooperation of the controllable degradation base material and the high-activity functional component, precise matching of the degradation rate and the bone regeneration period is achieved.