52 results about "Biogenic peptide" patented technology

The invention discloses a construction method of a microbial peptide mass fingerprint spectrum database based on a time-of-flight mass spectrometry principle, wherein the method comprises the steps: carrying out pure culture of obtained strains according to physiological habits, and successively obtaining strains at logarithmic phase; identifying the strains through morphology, taxonomy and methodology; then pretreating the confirmed strains, drying, and then extracting peptide fingerprint spectra by an ethanol/formic acid method and an 80% TFA extraction method; collecting and treating the spectra by adopting an MALDI-TOF MS platform, then carrying out parameter determination of the strain spectra, unifying the determined standard spectra and strain names, labeling a database construction method of the strains, and completing the database construction of the strains; and analogizing by the construction methods of the other strains successively, and completing the construction of the microbial peptide mass fingerprint spectrum database. The invention provides a set of complete method from the investigation of the strains to the construction of the database. The database has complete types of microorganisms, and meets identification requirements of microorganism related industries.

The invention discloses a nano carrier capable of pH-responsive rapid release of object molecules, and a preparation method and an application thereof. The nano carrier is prepared by carrying out a reaction of biological peptide or biological amino acid with a crosslinking agent; the biological peptide includes diphenylalanine and/or diphenylalanine carboxyl terminal amidated hydrochloride; the biological amino acid includes phenylalanine; the crosslinking agent comprises aliphatic dialdehyde. The preparation method comprises the following steps: mixing a biological peptide solution or a biological amino acid solution and a crosslinking agent solution, carrying out a Schiff base reaction, to obtain the nano carrier capable of pH-responsive rapid release of the object molecules. The nano carrier has the advantages of adjustable particle size, good stability and high encapsulation capability on medicines, and is capable of pH-responsive super rapid release.

The invention discloses traditional Chinese medicine gel for resisting oxidation and repairing gynecological diseases and a preparation method thereof, and relates to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 1-3 parts of gold foil, 1-3 parts of saussurea involucrata extract, 3-8 parts of collagen peptide, 5-10 parts of biological peptide, 3-8 parts of propolis, 2-5 parts of radix sophorae flavescentis, 2-5 parts of cortex phellodendri, 1-3 parts of fructus kochiae, 1-3 parts of fructus cnidii, 5-10 parts of aloe, 2-5 parts of ethylparaben and 10-20 parts of purified water. According to the traditional Chinese medicine gel for resisting oxidation and repairing gynecological diseases and the preparation method thereof, the traditional Chinese medicine gel can be used for daily bacteriostatic nursing, is free of dependence, can be used for preventing the gynecological diseases, has better anti-oxidation and bacteriostatic effects, and can maintain and repair the ecological balance of vagina and maintain the health of the vagina.

The invention discloses a production method of novel organic selenium-enriched bean sprouts. The production method comprises the following steps of disinfection, specifically, removing impurities and imperfect grains from soybeans, immersing the soybeans in a sodium hypochlorite solution with the mass percent concentration of 0.5-1.0% for disinfection for 5-30 minutes, and then washing the soybeans with clear water, preparing a nutrient solution, specifically, dissolving a selenium-containing polypeptide solution into water, and preparing an organic selenium solution with the selenium content of 1-5mg/L according to a proportion for later use, soaking, specifically, soaking the disinfected soybeans in the nutrient solution, wherein the ratio of the volume of the nutrient solution to the mass of the soybeans is (2.5-5): 1, and culturing, specifically, placing the soybeans in a seedling culture device, and culturing under a constant-temperature dark condition. The organic biological peptide-selenium chelate prepared by chelating polypeptide and inorganic selenium is used as a selenium source, so that the organic biological peptide-selenium chelate is absorbed by the soybeans in the growth process and is safe and non-toxic, and meanwhile, the polypeptide can effectively supplement amino acid and protein and enhance the nutrition of the bean sprouts.

The invention discloses composite functional male silkworm moth powder and an application thereof and belongs to the technical field of food and medical processing. The composite functional male silkworm moth powder is prepared by the following method: taking whole male silkworm moths, drying, crushing and collecting male silkworm moth cream; adding a bio-peptide or earthworm protein, an emulsifier, glucose syrup and water into the collected male silkworm moth cream for emulsification and homogenization to obtain a male silkworm moth suspension; and drying the suspension after emulsification and homogenization, crushing solids obtained after drying, completely screening and collecting. According to the composite functional male silkworm moth powder disclosed by the invention, the bio-peptide or earthworm protein is selected as an embedding agent, the prepared composite functional male silkworm moth powder can effectively retain the efficacy of a male silkworm moth finished medicine, effectively protect nutrients and active ingredients of the male silkworm moths from losing, and simultaneously effectively control the oil yield and the water yield on the surface of the male silkwormmoth powder, and can be be applied to the field of food or medicine.

The invention discloses a biological peptide solid state fermentation method for an animal feed. The biological peptide solid state fermentation method comprises the following steps of step S1, weighing raw materials; S2, performing mixing and stirring; S3, performing ultrasonic treatment; and S4, fermenting the raw materials in parts by mass of 15-25 parts of amino acids, 20-30 parts of agar, 15-25 parts of glucose, 10-20 parts of antioxidants, 2-4 parts of yeast cream, 10-20 parts of active protein, 1-3 parts of marine products, 5-10 parts of probiotics, 0.5-1 part of digestive enzymes and 0.5-1 part of polysaccharide. The invention further discloses a biological peptide solid state fermentation device for the animal feed. The amino acids are used as the raw materials for fermentation, the marine products provide organic trace elements, the probiotics facilitate protection of intestinal tract health, the digestive enzymes provide feed conversion rate, the polysaccharide can provide energy and growth factors quickly for organisms, a reaction is performed through ultrasonic waves, and the biological peptide for the animal feed is uniform in concentration during making.

The invention discloses a meat duck starter feed, which is composed of the following components by weight: 35-40 parts of corn, 5-8 parts of wheat bran, 8-10 parts of millet, 20-35 parts of soya bean meal, 1-2 parts of protein powder, 0.5-1.2 parts of stone powder, 0.3-0.8 part of calcium hydrogen phosphate, 0.3-0.5 part of glutamine, 8-10 parts of Gorgon fruit, 0.2-0.4 part of lysine, 0.2-0.4 part of salt, 0.2-0.3 part of glucose, 0.1-0.3 part of methionine, 2-4 parts of edible oil, 2-4 parts of biologic peptide, 5-8 parts of fish meal, 2-4 parts of housefly larvae protein powder, 2-4 parts of earthworm protein powder, 1-3 parts of silkworm chrysalis powder, 2-4 parts of hawthorn powder, 5-8 parts of algae powder, 0.5-1.0 part of ginger powder, and 3-8 parts of Chinese herbal medicines. starter meat duck fed with the meat duck starter feed provided by the invention has low feed conversion rate and reduced mortality, thereby reducing the breeding cost and increasing the breeding benefit.

The invention discloses polypeptide nursing antibacterial gel and a preparation method thereof, and relates to the technical field of antibacterial drugs. The polypeptide nursing antibacterial gel isprepared from antibacterial raw materials. The antibacterial raw materials comprise ecological balance liquid and plant extracts, and the ecological balance liquid is prepared from 5-12 parts of biological peptide, 0.1-0.4 part of collagen, 15-27 parts of plant active polysaccharide, 9-11 parts of purified water and 3-8 parts of geranium essential oil; the plant extract comprises radix sophorae flavescentis, cortex phellodendri, fructus cnidii, radix salviae miltiorrhizae, flos carthami, herba leonuri and folium artemisiae argyi; the antibacterial raw materials further comprise 0.2-0.3 part oflactic acid, 0.6-1.8 parts of a pH regulator, 1.5-3.5 parts of a gel matrix and 40-45 parts of purified water. The polypeptide nursing antibacterial gel has the advantage of good environmental protection performance. The preparation method comprises the following steps: mixing and dissolving, medicine extraction, water swelling, dilution, gelling and the like. The preparation method has the advantage that the treatment effect of the product can be improved.

The invention provides a skin micro-ecological balance composition. The skin micro-ecological balance composition is prepared from the following components in parts by mass: 0.01 to 5 parts of probiotics, 0.01 to 10 parts of prebiotics, 0.1 to 2 parts of cell energy biological peptide, 0.01 to 1 part of copper tripeptide-1, 0.1 to 10 parts of vitamin X and 0.01 to 1 part of dipotassium glycyrrhizinate. The invention belongs to the technical field of cosmetics, oligopeptide polypeptides are innovatively matched with prebiotics and probiotics to construct micro-ecological balance, and meanwhile, the oligopeptide polypeptides are matched with copper tripeptide-1, vitamin X and dipotassium glycyrrhizinate to generate a synergistic effect, so that the skin barrier can be tough, the skin can be moisturized for a long time, irritation can be relieved, sensitive skin can be repaired, and the micro-ecological balance of the skin can be maintained.

The invention provides a targeted biological peptide for inhibiting autoimmune inflammatory response of eyes and application of the targeted biological peptide. The invention also relates to a preparation method and application of the polypeptide and a pharmaceutical composition containing the polypeptide. The polypeptide disclosed by the invention has various advantages, such as small molecular weight, low production cost, good water solubility, small immunogenicity, low toxic and side effects, strong tissue penetrability and the like; moreover, researches on two models, namely EAE and EAU, show that the biological peptide has remarkable effects of immunoregulation, inflammation resistance and visual function improvement.

The invention discloses a biomimetic multifunctional lipoprotein nanoparticle modified by biological peptide and a preparation method and application thereof, the multifunctional biomimetic lipoprotein nanoparticle is composed of a biomimetic lipoprotein nano-carrier shell modified by biological peptide and a nano-form drug core, and the carrier shell is composed of apolipoprotein bionic peptide, biological peptide and natural phospholipid substitute. The preparation method comprises a film dispersion method and an emulsification evaporation method. The preparation method disclosed by the invention is simple and mild in condition and low in cost, and the prepared biological peptide modified biomimetic multifunctional lipoprotein nanoparticle has the advantages of high drug loading capacity, high biomimetic property, strong penetrability, biological safety, lesion site targeting property, drug loading mode diversity and the like. A nano-drug delivery platform can realize 'diagnosis and treatment integration' and 'multi-mode combined treatment', and can realize timely diagnosis and efficient treatment of complex progressive diseases such as tumors, neurodegenerative diseases and the like.

The invention discloses a gel preparation for effectively conditioning and preventing HPV (human papillomavirus) in gynecology and a preparation process, and relates to the technical field of medicines. The preparation method comprises the following steps: mixing the cleaned fructus cnidii, radix sophorae flavescentis, cortex phellodendri, fructus kochiae, folium artemisiae argyi and flos carthami, crushing into powder, sieving, adding purified water, heating, filtering, cooling, adding the collagen peptide, the biological peptide and the purified water into the filtrate, heating, filtering and concentrating to obtain an extract, and drying to obtain the collagen peptide biological peptide. And mixing the extract with fully dissolved carbomer, menthol, methylparaben and purified water, uniformly stirring, emulsifying to form gel, filling the gel after the gel is detected to be qualified, filling the gel into a drug delivery device, and sealing the drug delivery device. The traditional Chinese medicine composition is simple and convenient in preparation process, has a relatively good prevention and treatment effect on HPV, is free of toxic and side effects, realizes a good inhibition function on HPV viruses, can enhance the anti-infection ability of a human body and the immunity of the organism, has a good anti-virus effect, and has a wide application prospect.

A two-layer gradient coating article is provided that is operable to cause selective adhesion and directional migration of endothelial cells. The first layer includes cell-resisting polymers that repels cells, the second layer includes one layer of peptides that has affinity to and binds specifically to endothelial cells. Furthermore, the peptides are distributed in a gradient in which attached ECs migrate towards the direction of increased concentration, thus enriching the ECs to a desired locus. The combination of a cell-repelling layer and a graded affinity peptide produces a unique result of selective adhesion, directional migration, thus local enrichment of endothelial cells. A method for using such gradient coating article and its potential use in treating cardiovascular diseases are also provided. The invention provides an inexpensive, stable and effective means for attracting ECs to desirable locations.

The invention discloses the technical field of wet tissue preparation, and particularly relates to an antibacterial and antiviral wet tissue and a preparation method thereof. A silver ion antibacterial agent is prepared in the mode that metal silver simple substance with the particle size reaching the nanoscale is adopted, and silver ions are put into a carrier to be made into a solution, the silver ions are subjected to a contact reaction, consensus components of microorganisms are damaged or dysfunction is generated, when a trace amount of silver ions reach a microbial cell membrane, the microbe carries negative charges, the microbe and the silver ions are firmly adsorbed by coulomb attraction, and the silver ions penetrate cell walls to enter cells, so that proteins are solidified, the activity of cell synthetase is destroyed, and the cells lose division and multiplication capacities to die; the silver ion antibacterial agent and the composite double-chain quaternary ammonium salt disinfectant are matched for use, so that the disinfection component has antibacterial and antiviral capabilities at the same time; and the biological peptide antibacterial gel and the polylysine antibacterial agent are matched for use, the synergistic antibacterial and antiviral effects are achieved, and the polylysine antibacterial agent has a certain preservative effect, is a natural preservative and can enable the disinfection component to keep the disinfection activity for a long time.

The invention belongs to the technical field of biological fertilizers, and particularly relates to an enzymolysis biological peptide fertilizer and a preparation method thereof. The enzymolysis biological peptide fertilizer is prepared from the following raw materials in parts by weight: 4 to 12 parts of poultry meat, 2 to 6 parts of livestock meat, 1 to 5 parts of water, 1 to 5 parts of proteolytic enzyme, 0.4 to 2.5 parts of finely ground garlic powder and 1 to 5 parts of garlic enzyme. By utilizing the activity of proteolytic enzyme, meat of poultry and livestock which are overdue and deteriorated and are not suitable for human being to eat any more is degraded into amino acid and molecular peptide type aqueous solutions, and then water-soluble compounding of allicin is carried out in the later processing process, so that the allicin can be quickly absorbed by plants in agricultural planting, and the bacteriostatic, antibacterial and bactericidal effects of allicin can be utilized to bring a certain protection effect to the plant root system. By adopting the method, the use quantity of chemical fertilizers and pesticides can be reduced, and the plants cannot generate pesticide resistance, so that the aims of saving cost and increasing production and income are fulfilled.

The invention relates to the field of medicine, in particular to novel peptides having biocidal properties, more particularly antibacterial, antifungal and antiviral properties, and preparations based thereon. Proposed are a biocidal peptide of general formula Y-Ile-Leu-Pro-X-Lys-X-Pro-X-X-Pro-X-Arg-Arg-NH2, where X is 4-nitrophenylalanine; 4-chlorophenylalanine; 4-methoxyphenylalanine; D-phenylalanine; 4-aminophenylalanine; 4-aminobenzoylphenylalanine; homophenylalanine; 4-tertbutylphenylalanine; 2-methylphenylalanine; 4-fluorophenyl alanine; pentafluorophenylalanine; or 2-trifluoromethylphenylalanine; and Y is H or palmitoyl or aminodecanoyl, and a preparation in the form of a gel with biocidal properties, containing, as an active substance, said peptide at a concentration of 0.001 to 0.1 wt %. The claimed peptides demonstrate biocidal properties towards bacteria, including spore-forming bacteria, mould fungi, and also viruses. The peptide-based gels can be used for treating bacterial and viral infectious diseases and infectious comorbidities.

The invention discloses a biological peptide preparation capable of lowering blood fat, and a preparation technology of the biological peptide preparation. The biological peptide preparation consistsof 30-70 parts of Zea mays L., 20-60 parts of Avena sativa L., 300-500 parts of milk, 50-90 parts of Allium cepa L., 100-160 parts of semen armeniacae amarum, 15-35 parts of flos chrysanthemi, 40-90 parts of egg white, 240-460 parts of Glycine max (Linn.) Merr., 35-75 parts of Lentinus edodes (Berk.) sing, 15-55 parts of fishskin, 70-120 parts of Momordica charantia L., 300-650 parts of soybean, 26-52 parts of fructus crataegi, 32-66 parts of Actinidia chinensis Planch, 40-75 parts of traditional Chinese medicine preparations and 35-80 parts of biological modifying agents. Since the traditional Chinese medicine preparations are added into raw materials, the biological peptide preparation has excellent performance of lowering blood fat, in addition, the biological peptide preparation contains a great quantity of ingredients capable of lowering blood fat, lowering serum cholesterol, lowering fat, resolving turbidity, invigorating the circulation of blood, promoting circulation of qi, preventing and treating atherosclerosis, promoting blood circulation, replenishing qi and blood and the like, and the biological peptide preparation is simple to operate and can meet the blood fat lowering requirements of people.

The invention provides a biological peptide for treating asthma and application of the biological peptide. The invention also relates to a preparation method and application of the polypeptide and a pharmaceutical composition containing the polypeptide. The polypeptide disclosed by the invention has the advantages of small molecular weight, low production cost, good water solubility, good stability, long half-life period, small immunogenicity, low toxic and side effects, strong tissue penetrability and the like; and moreover, the research on an OVA-induced asthma mouse model shows that the biological peptide has remarkable functions of preventing, treating and/or relieving asthma.

The invention discloses a preparation method of marine fish biological peptide, which comprises the following steps: 1) taking fresh marine fish meat, and adding water to prepare fish slurry; cooking for 20-30 minutes, and cooling to obtain homogenate; 2) adjusting the pH value of the homogenate to 5-7, adding protease and an enzyme activator, carrying out enzymolysis at 48-52 DEG C, and carrying out enzyme deactivation to obtain an enzymatic hydrolysate; 3) performing microfiltration on the enzymatic hydrolysate through a ceramic membrane, performing graded purification through an organic ultrafiltration membrane, and performing nanofiltration through a nanofiltration membrane; 4) drying the filtrate obtained in the step 3) by a freeze-drying method to obtain primary marine fish biological peptide; and 5) removing bitterness and fishy smell of the preliminary marine biological peptide prepared in the step 4). The invention belongs to the technical field of preparation of marine fish oligopeptides, and particularly relates to a preparation method of a marine fish biological peptide which is used for removing bitterness and fishy smell of the marine fish biological peptide and improving the taste, the obtained marine fish biological peptide is stable in property and high in active ingredient content, and meanwhile, the preparation method is simple in technological process, easy to operate and low in cost.

The invention provides a recovery method of low-value fish processing by-products. The recovery method comprises the following steps: (1) adding the low-value fish processing by-products into water, performing uniform stirring to obtain a mixed solution, regulating the pH value of the mixed solution to 6-7, adding compound protease into a vacuum environment, performing heating to 40 DEG C, performing enzymolysis for 3-5 hours to obtain enzymatic hydrolysate, heating the enzymatic hydrolysate for enzyme deactivation, performing cooling, cooling filtering to obtain filtrate, and drying the filtrate to obtain zymolyte; and (2) crushing the zymolyte, sieving the crushed zymolyte with a 80-mesh sieve to obtain zymolyte powder, stirring the zymolyte powder, the zymophyte and water until the materials are uniformly mixed to obtain a mixed material, performing sealed fermentation on the mixed material at 35 DEG C for 50-60 hours to obtain a fermented material, and performing vacuum concentration and drying on the fermented material to obtain the biological peptide. According to the invention, the low-value fish processing by-products can be converted into the biological peptide with excellent feeding value and nutritional value.

The invention provides a method for high-throughput screening of ACE inhibitory peptides, and relates to the technical field of preparation and screening of biological peptides. The method comprises the following steps: 1) preparing a protein hydrolysate; 2) determining the optimal screening concentration of hydrolysate; (3) carrying out biological affinity ultrafiltration; and 4) performing high-throughput identification on peptide composition and performing molecular docking secondary screening by taking binding energy as an index. According to the method, biological affinity ultrafiltration is combined with a high-throughput identification technology, so that the screening efficiency is greatly improved; the screening accuracy is improved to the maximum extent on the basis of the biocompatibility of interaction of the micromolecule ligand and the enzyme; the consumption of enzymes and samples in the whole screening process is greatly reduced, and the ACE inhibitory activity of the peptide can be predicted and the binding site of the peptide and ACE can be analyzed by molecular docking simulation screening taking binding energy as an index; and the method disclosed by the invention has the advantages of high screening efficiency, short time consumption and high accuracy, and can be widely applied to screening of the ACE inhibitory peptide.