55 results about "Biogenic peptide" patented technology

The invention relates to the field of preparation and application of biological peptides, in particular to a hippocampal swelling peptide and application thereof in preparation of functional products for repairing male reproductive system injury. A green controllable enzymolysis technology is utilized to obtain protein polypeptide with nutritional, health-care and medicinal values, five active peptide sequences with high biological activity are obtained through further research on the basis, the high-purity active peptide is obtained through a chemical synthesis mode, and the biological activity of the peptide is verified through mouse spermatogonium.

The invention relates to the field of preparation and application of biological peptides, in particular to a swelling hippocampal peptide and application of the swelling hippocampal peptide in preparation of anti-fatigue products. Five active peptide sequences with high biological activity are obtained through research, animal behavioral experiments prove that the belly distention hippocampal peptide has a remarkable anti-fatigue effect and can prolong the swimming exhaustion time and enhance the muscle strength, the anti-fatigue effect of the belly distention hippocampal peptide is verified, and a new way is provided for development of anti-fatigue hippocampal products.

The invention relates to the field of preparation and application of biological peptides, in particular to a swelling hippocampal peptide and application of the swelling hippocampal peptide in preparation of a product for improving memory ability and cognitive impairment. According to the invention, a green controllable enzymolysis technology is utilized to obtain protein polypeptide which has the main molecular weight of less than 1000 Da and has nutritional, health-care and medicinal values, and four active peptide sequences with high biological activity are obtained by further research on the basis of the protein polypeptide.

The invention provides a multifunctional self-assembly peptide recognition method and system, and relates to the technical field of biological peptide recognition. The method comprises the following steps: firstly, obtaining peptide sequences of a self-assembled peptide and a plurality of biological functional peptides, and inputting the peptide sequences into an embedded layer of a polypeptide function prediction model for 21-dimensional vectorization; and learning combination modes and sequence features of amino acids through an amino acid association feature extraction layer of the LSTM network. Then, acquiring direct contact information among different amino acids on the basis of a fine-grained feature learning layer of a multi-head self-attention mechanism; and finally, designing a Transform multi-task classifier which comprises five sub-task classifiers which are used for respectively predicting the self-assembly function, the anti-cancer function, the anti-inflammatory function, the antibacterial function and the antioxidant function. According to the method, a multi-layer structure is adopted to carry out layer-by-layer deep learning and optimization on polypeptide sequence features, efficient recognition and classification of multifunctional biological peptides are achieved, and finally the model performance is comprehensively evaluated through the accuracy rate, the precision rate, the recall rate and the F1 value.

The invention relates to the field of preparation and application of biological peptides, in particular to a swelling hippocampal peptide and application thereof in preparation of uric acid reducing and gout resisting products. The amino acid sequence is shown as SEQ ID NO.1. The uric acid reducing effect of the compound is proved through in-vitro xanthine oxidase (XOD) and adenosine deaminase (ADA) inhibition experiments and cell experiments; on the basis, the uric acid reducing and gout resisting activity of the peptide sequence is further verified by means of a hyperuricemia animal model. The achievement provides a solid theoretical support for high-value development and utilization of the hippocampus japonicus and research and development of uric acid reducing and gout resisting related products.

The invention discloses a corn peptide for dispelling effects of alcohol and protecting liver as well as a preparation method and application thereof, and belongs to the technical field of biological peptide extraction. The preparation method comprises the following steps: crushing and sieving corn, dissolving the corn in deionized water, adjusting the pH value to be alkaline, and performing ultrasonic-assisted extraction to obtain corn homogenate; centrifuging, taking supernate, adjusting the pH to be neutral, centrifuging, adding an ethanol solution with the mass fraction of 10%, standing, carrying out continuous flow centrifugation, suspending precipitates in distilled water, dialyzing in a dialysis bag, and freeze-drying to obtain crude corn protein; the preparation method comprises the following steps: preparing an aqueous solution, carrying out variable-temperature treatment, adjusting the pH value to be alkaline, adding trypsin and alkaline protease, carrying out enzymolysis, repeating the enzymolysis twice, and after the enzymolysis is finished, carrying out heating enzyme deactivation, centrifugation, sediment discarding, ultrafiltration, centrifugation, supernatant taking and spray drying to obtain the corn peptide. The corn peptide can remarkably shorten the drunkenness time and sobering time of mice and reduce the drunkenness duration, and has good effects of dispelling the effects of alcohol and protecting the liver.

The invention relates to the field of preparation and application of biological peptides, in particular to a swelling hippocampal peptide and application thereof in preparation of uric acid reducing and gout resisting products. The amino acid sequence is shown as SEQ ID NO.1-5. The uric acid reducing effect is verified by adopting an in-vitro XOD inhibition experiment and a cell experiment; furthermore, the uric acid reducing and gout resisting activity of the compound is verified through a hyperuricemia animal model, and a theoretical basis is provided for high-valued utilization of the belly-swelling hippocampus and development of uric acid reducing and gout resisting products.

The invention belongs to the technical field of biological medicine, and particularly relates to peptide slow-release temperature-sensitive hydrogel as well as a composition, a preparation method and application thereof. The composition consists of a hydrogel monomer polymer and biological peptide, wherein the biological peptide is Leucokinin VIII, and the biological peptide is Leucokinin VIII The hydrogel monomer polymer is prepared by mixing and heating polyethylene glycol, lactide and stannous zincate. The preparation method of the peptide slow-release temperature-sensitive hydrogel comprises the following steps: mixing polyethylene glycol, lactide and stannous zincate, and carrying out heating and stirring reaction to prepare a hydrogel monomer polymer; and then mixing the hydrogel monomer polymer with the Leucokinin VIII, so as to obtain the peptide slow-release temperature-sensitive hydrogel. The peptide sustained-release temperature-sensitive hydrogel has the advantages of excellent temperature-sensitive characteristic, injectability, drug sustained-release performance, biocompatibility and biological safety.

The invention relates to the technical field of antioxidant peptides, in particular to an antioxidant peptide HYHE derived from dietary protein and application of the antioxidant peptide HYHE. The amino acid sequence of the antioxidant peptide is HYHE and is as shown in SEQ ID NO: 1. The antioxidant peptide is obtained based on prediction of an antioxidant peptide prediction platform Pred5AOP; 27 dietary proteins are virtually hydrolyzed through trypsin and pepsin, and the antioxidant peptide HYHE is screened out; compared with chemical drugs, the antioxidant peptide HYHE serving as the biological peptide has the advantages of high stability, low immunogenicity, high purity and the like; meanwhile, the invention further proves that the dietary protein has rich nutritional value, provides a new thought for resource recycling, and has good social and economic benefits.

The invention provides antibacterial shampoo for preventing infection and a preparation method thereof.The antibacterial shampoo is composed of 5-10 wt% of active ingredient composition and 90-95 wt% of auxiliary materials, the active ingredient composition comprises a component A and a component B with the mass ratio being 2-4: 1, and the component A comprises 2-4 parts of agilawood micro-emulsion, 2-4 parts of morinda citrifolia fruit micro-emulsion, 1-3 parts of ganoderma lucidum fermentation liquor and 1-3 parts of biological peptide; the component B is prepared from 1 to 3 parts of saponin extract, 1 to 3 parts of cacumen biotae extract, 1 to 3 parts of radix polygoni multiflori extract, 1 to 3 parts of radix ginseng extract, 0.8 to 1.2 parts of herba houttuyniae extract, 2 to 4 parts of radix sophorae flavescentis extract, 2 to 4 parts of fructus viticis extract and 0.8 to 1.2 parts of herba ecliptae extract; the auxiliary materials comprise the following components in parts by weight: 4-6 parts of chitosan / cationic guar gum composite gel, 0.2-0.4 part of a thickening agent, 1-3 parts of a surfactant and 500-600 parts of water. According to the shampoo disclosed by the invention, the active components are compounded, so that efficient and mild bacteriostasis is realized, infection after hair transplantation is prevented, wounds can be quickly repaired, scalp can be protected, and the shampoo is safe and non-irritant and has popularization value.

The invention belongs to the technical field of biological peptide preparation, and particularly relates to a globin peptide stock solution feeding and mixing device for peptide copper preparation. Comprising a stirring tank, the top of the stirring tank is connected with a protective shell, and the bottom end of the stirring tank is connected with a discharge port; the stirring tank is connected with a quantitative feeding mechanism, and the protective shell is connected with a stirring and flapping mechanism; the quantitative feeding mechanism comprises a feeding box, a rotating disc and a discharging disc which are sequentially arranged from top to bottom. The stirring and beating mechanism comprises a motor, a second rotating shaft and a beater; bubbles on the surface of mixed liquid can be easily damaged through the stirring and flapping mechanism, the blockage problem is not prone to occurring when the quantitative feeding mechanism is installed, uniform feeding and mixing can be achieved through cooperation with stirring, and the requirement for quantitative feeding can be continuously met; the problems existing in the protein feeding process are solved.

The present invention relates to the preparation and application of biological peptides, and in particular to a mussel peptide and its application in the preparation of uric acid-lowering products. The amino acid sequence is shown in SEQ ID NO.1‑4. The present invention prepares mussel polypeptides by composite enzyme-directed enzymatic hydrolysis and membrane separation and purification technology, and screens out four novel polypeptides with potential biological activity by combining LC‑MS / MS analysis and activity prediction. Molecular docking and cell experiments have confirmed that these four polypeptides can specifically inhibit GLUT9 protein activity and reduce uric acid levels; animal experiments have further verified their efficacy in lowering uric acid, providing a scientific basis for the high-value development of mussel resources and the development of functional uric acid-lowering products.

The present application relates to the preparation and application field of biological peptide, and more particularly to a hippocampus abdominalis peptide and its application in preparation of products for reducing uric acid and resisting gout. The amino acid sequence is shown as SEQ ID NO. 1, and the present application confirms the uric acid reducing effect through in vitro xanthine oxidase (XOD) and adenosine deaminase (ADA) inhibition experiments and cell experiments; on this basis, the uric acid reducing and gout resisting activity of the peptide sequence is further verified by means of a high uric acid animal model. This achievement provides a solid theoretical support for high-value development and utilization of hippocampus abdominalis, and research and development of products for reducing uric acid and resisting gout.

The invention relates to the field of preparation and application of biological peptides, in particular to a swelling hippocampal peptide and application of the swelling hippocampal peptide in preparation of a product for improving memory ability and cognitive impairment. According to the invention, a green controllable enzymolysis technology is utilized to obtain the protein polypeptide with the main molecular weight of less than 1000 Da and nutritional, health-care and medicinal values, further research is carried out on the basis to obtain an active peptide sequence with high biological activity, and the medicinal value of the active peptide sequence is verified.

This invention belongs to the field of biopeptide extraction technology, specifically relating to a water lily polypeptide, its extraction method, and its application. The invention involves sequentially disinfecting, washing, drying, and pulverizing the water lily sample to remove impurities from its surface. Heating the pulverized sample with water facilitates the initial precipitation of active ingredients from the water lily. Adding papain for enzymatic hydrolysis and then using an ethanol solution for analysis helps to penetrate the water lily sample, fully releasing the internal water lily polypeptides while maintaining high activity. Therefore, the water lily polypeptide provided by this invention has high activity and can be used in anti-wrinkle and whitening products.

The invention relates to the technical field of bioinformatics, and discloses a biological peptide activity data analysis method and system, and the method comprises the steps: carrying out the embedded vector construction of an input biological peptide sample; on the basis of the embedded representation, in combination with a preset candidate model set, calculating an adaptation probability between each model and the sample; constructing a path diagram structure by taking the model as a node, introducing a model switching cost function, and jointly optimizing a reasoning path based on the adaptation probability and the cost; executing a reasoning process of the model in the path, and completing sequence transmission and scheduling control of an intermediate result; and finally integrating all model outputs to generate a biological peptide activity prediction result. The system supports dynamic path planning, inference process fault tolerance and execution optimization, and the precision and efficiency of biological peptide analysis are improved. According to the method, the problems of single model adaptation, low resource utilization and unstable execution process in the existing method are effectively solved, and the method has good universality and expansion capability.

The invention discloses an intestine-moistening and stomach-protecting wheat peptide and a preparation method and application thereof, belongs to the technical field of biological peptides, and particularly relates to a preparation method of the intestine-moistening and stomach-protecting wheat peptide, which comprises the following steps: pre-mixing wheat gluten protein with water to obtain a wheat gluten protein aqueous solution; dropwise adding a NaOH aqueous solution, centrifuging, and taking supernate; adding citric acid to adjust the pH value, standing, and taking a precipitate; dissolving the precipitate in water to obtain a protein solution, adding a compound enzyme preparation, carrying out enzymolysis to obtain a peptide solution, centrifuging, taking a supernatant to obtain a wheat peptide solution, adding mannitol, then adding glycine, and carrying out ultrafiltration and freeze drying to obtain wheat peptide; the compound enzyme preparation comprises ProteAX enzyme, Alcalase protease and EDTA (Ethylene Diamine Tetraacetic Acid). By adjusting the preparation method and matching with the special compound enzyme preparation prepared from ProteAX enzyme, Alcala enzyme protease and EDTA, the intestine moistening and stomach protecting effects of the prepared wheat peptide are improved.

The invention belongs to the technical field of biological peptides and application, and particularly relates to an antioxidant peptide with a Keap1 inhibiting effect and application of the antioxidant peptide. The antioxidant peptide provided by the invention comprises antioxidant pentapeptide, antioxidant tripeptide and the like which all show good in-vitro antioxidant activity, the scavenging rate of ABTS free radicals reaches 0.69-1.11 [mu] mol TE / [mu] mol, the scavenging rate of DPPH free radicals reaches 30.11%-34.94%, in addition, the antioxidant peptide can activate the expression of Nrf2 and downstream genes HO-1 and NQO1 thereof by inhibiting the expression level of Keap1 protein, so that the antioxidant activity of the antioxidant peptide is improved. Therefore, the compound has a wide application prospect in the fields of cosmetics, medicines, foods and the like.

The invention relates to the technical field of high-value utilization of marine biological resources, and discloses a high-activity marine biological peptide prepared by an enzymolysis method and application of the high-activity marine biological peptide in food. The target high-activity marine biological peptide and an affinity molecular switch sensitive to the concentration change of the target high-activity marine biological peptide are jointly fixed on a magnetic nano-carrier with bitter peptide adsorption functional groups on the surface to obtain the intelligent enzyme carrier; and adding the intelligent enzyme carrier into a reaction system containing a marine protein raw material and a first amphiphilic microcapsule wall material to carry out an enzymatic hydrolysis reaction in the reaction process. By constructing an intelligent enzyme carrier integrated with a directional enzyme and an affinity molecular switch, precise cutting and process self-control of an enzymolysis reaction are realized, and the affinity molecular switch can respond to the change of the concentration of the high-activity marine biological peptide and spontaneously adjust the enzyme activity, so that excessive degradation of a target product is effectively avoided.

This invention discloses an abalone-derived xanthine oxidase inhibitory peptide and its application, belonging to the field of biopeptides. Using abalone as raw material, this invention employs a mild blanching-ultra-high pressure-ultrasound synergistic pretreatment process, followed by enzymatic hydrolysis, separation and purification, and bioinformatics screening combined with molecular docking technology to obtain four polypeptides with amino acid sequences as shown in SEQ ID NO. 1-4. Experimental results show that the abalone-derived polypeptides provided by this invention have outstanding XOD inhibitory activity, with a half-maximal inhibitory concentration as low as 0.53 mg / mL, superior to existing shellfish peptides. They are non-toxic or non-sensitizing and can effectively reduce uric acid and pro-inflammatory factor levels in a hyperuricemia cell model, and can be applied to the preparation of uric acid-lowering drugs. This invention effectively overcomes the shortcomings of low activity and limited sources of natural peptides, promotes the high-quality utilization of abalone protein resources, improves the level of deep processing in the abalone industry, and provides novel natural candidate substances and technical solutions for the prevention and treatment of hyperuricemia.

The invention belongs to the technical field of biological peptide processing, and particularly discloses oyster peptide for improving male functions and a preparation method of the oyster peptide. The preparation method comprises the following steps: S1, mixing fresh oyster meat with an algae substance, adding deionized water, grinding, adjusting the pH value to 6.5-7.0, and carrying out ultrasonic treatment to obtain a homogenate; s2, adding compound protease into the homogenate obtained in the step S1, and performing enzymolysis to obtain enzymatic hydrolysate; s3, adding an organic matter into the enzymatic hydrolysate obtained in S2, and heating for reaction to obtain a reaction solution; s4, adjusting the pH value of the reactant obtained in S3 to 5.5-6.5, adding lactic acid bacteria, and fermenting to obtain fermentation liquor; and S5, performing inactivation and ultrafiltration on the fermentation liquor obtained in the step S4 to obtain the oyster peptide. The invention discloses an oyster peptide capable of improving male functions and a preparation method thereof. The oyster peptide obtained by adopting the preparation method is high in yield, free of fishy smell and capable of remarkably enhancing male function activity.

The invention belongs to the technical field of biological peptide enzymolysis, and particularly discloses a sheep three-medullary peptide composition for assisting in improving memory and application of the sheep three-medullary peptide composition. The sheep three-medullary peptide composition is prepared from the following components in parts by mass: 40 to 50 parts of sheep three-medullary peptide, 20 to 35 parts of sheep colostrum peptide and 10 to 25 parts of casein hydrolyzed peptide. The invention discloses a sheep three-medullary peptide composition for assisting in improving memory and application thereof, which can obviously improve the level of acetylcholine in the brain and inhibit the activity of acetylcholin esterase to enhance nerve conduction, and can effectively relieve the damage of oxidative stress to brain tissues by improving the activity of SOD and GSH-Px and reducing the content of MDA.

The invention relates to the field of preparation and application of biological peptides, in particular to a mussel peptide and application thereof in preparation of uric acid reducing products. The amino acid sequences of the gene are as shown in SEQ ID NO.1-4. The mussel polypeptide is prepared through directional enzymolysis of a compound enzyme and a membrane separation and purification technology, and four novel polypeptides with potential biological activity are screened by combining LC-MS / MS analysis and activity prediction. Molecular docking and cell experiments prove that the four polypeptides can specifically inhibit the GLUT9 protein activity and reduce the uric acid level; animal experiments further verify the effect of the mussel in the aspect of reducing uric acid, and a scientific basis is provided for high-value development of mussel resources and development of functional uric acid-reducing products.

The invention relates to the field of medicines, in particular to a biological peptide bacteriostatic tablet and a preparation method thereof. Comprising the following raw materials in parts by weight: 10-20 parts of biological peptide, 5-15 parts of chitosan, 5-10 parts of sodium alginate, 1-5 parts of glycerol, 2-8 parts of an antibacterial synergist, 1-3 parts of titanium dioxide and 0.5-2 parts of nano-silver particles. Titanium dioxide and nano-silver particles are introduced to jointly improve the antibacterial effect of the biological peptide antibacterial tablet.

The invention provides a method for extracting chitin and biological peptide based on waste insects, and relates to the technical field of biological enzymolysis. The method comprises the following specific steps: S1, pretreatment: removing impurities from waste insects, drying, and carrying out superfine grinding to obtain insect powder; s2, separation and purification: adding the insect powder in the S1 into deionized water, adding a compound enzyme, carrying out enzymolysis and solid-liquid separation, collecting a liquid phase, and washing a solid phase for later use; s3, preparing chitin: adding the solid phase in the S2 into an ethylenediamine tetraacetic acid solution, carrying out constant-temperature microwave-ultrasonic synergistic treatment, adjusting the pH value of the solution, adding chitin compound enzyme, and continuing constant-temperature microwave-ultrasonic synergistic treatment and solid-liquid separation to obtain the chitin as the solid phase; s4, preparing the biological peptide: concentrating the liquid phase in S2, separating through an ultrafiltration membrane to obtain a concentrated solution, and freeze-drying to obtain the biological peptide. The preparation method disclosed by the invention is mild in process conditions, does not need strong acid and strong alkali, does not cause pollution to the environment, and has good economic benefits and social benefits.

The invention provides an antioxidant biological peptide as well as a preparation method and application thereof. The modified sodium alginate is added into the antioxidant skin-care biological peptide composition, and the antioxidant biological peptide is combined into a double-crosslinking structure of the modified sodium alginate, so that better antioxidant effect and stability can be realized. The modified sodium alginate is formed through multi-step treatment, so that the problems that the traditional biological peptide is unstable in structure and is easily influenced by the environment to lose efficacy can be solved, and the action time of the biological peptide can be prolonged; the gallic acid has oxidation resistance and can be in synergistic interaction with the biological peptide, so that a foundation is laid for high-efficiency oxidation resistance of the composition. In the composition, the modified sodium alginate can improve the compatibility with other components and the system stability, and enhance the overall free radical scavenging ability; meanwhile, biological peptides are protected, activity attenuation in storage is reduced, the anti-degradation capacity of the composition is enhanced, and the problems that existing antioxidant cosmetics are poor in stability and poor in long-term use effect are solved.

The invention relates to foot powder containing a biological peptide composition, which comprises 3%-5% of a composite biological peptide composition, 50%-70% of an adsorbent, 1%-3% of an antifungal agent, 0.5%-2% of a cool soothing agent, 0.5%-1% of a pH regulator, 1%-5% of a natural additive, 1%-5% of essence, 0.5%-1.5% of ceramide and 2%-3% of a purslane extract, according to the foot powder containing the biological peptide composition and the preparation method of the foot powder, the biological peptide is added to inhibit common bacteria on feet in a targeted mode, the risk of drug resistance is reduced, the natural extracts are combined, the anti-inflammatory and itching-relieving effects are enhanced, a weak acid formula is adopted to be attached to the physiological environment of the skin, irritation to the skin is reduced, and the foot powder containing the biological peptide composition and the preparation method of the foot powder containing the biological peptide composition have the advantages of being free of toxic and side effects. And the ceramide and the purslane extract are added to protect the skin, and the independent biological peptide powder is adopted to enhance the treatment in the acute attack period, so that the experience feeling is improved while the anti-inflammatory and sterilizing effects are improved.

The invention belongs to the technical field of biochemistry, and particularly discloses anti-helicobacter pylori mouth wash, which is prepared from small molecule peptides of SEQ NO: 1-Trp-Cys-Glu-Arg-Met-His-Ile and SEQ NO: 2-Phe-Asp-Val-Lys-Tyr-Gln-Leu according to a specific proportion, 5-(2-aminopropyl)-1, 3, 4-thiadiazole-2-mercaptan (APTT), and auxiliaries such as purified water, ethanol and the like. The SEQ NO: 1 is targeted to helicobacter pylori cell membrane transporter, and cooperates with SEQ NO: 2 acting on DNA repair enzyme for bacteriostasis, APTT synergism is achieved, and biological peptide is stabilized. During preparation of the mouthwash, all the components are synthesized and purified firstly and then are mixed and stirred. The product has the advantages of high helicobacter pylori inhibition rate, long action time and good stability, can be used for daily oral care and prevention and adjuvant therapy of helicobacter pylori infection related diseases, has good biocompatibility, and has wide application prospects.

The present application relates to the preparation and application field of biological peptide, and particularly relates to a plectinum peptide and application of the plectinum peptide in preparation of a product for repairing male reproductive system damage function. The present application utilizes green controllable enzymolysis technology to obtain protein polypeptides with nutritional health care and medicinal values, and further researches on the basis to obtain an active peptide sequence with high biological activity, obtains the active peptide with high purity through a chemical synthesis mode, and verifies the biological activity of the peptide by using mouse spermatogonia.

The invention relates to the technical field of biological peptide processing, in particular to a raw material treatment device for biological peptide processing and a treatment process thereof.The inner sides of two supporting column plates are fixedly connected with two triangular prism plates correspondingly, the inner sides of the four triangular prism plates are fixedly connected with lower grinding blocks, and the inner sides of the two supporting column plates are fixedly connected with supporting plates; the inner side of the supporting plate is rotationally connected with an upper grinding block, the upper grinding block is located at the top of the lower grinding block, a feeding groove is formed in the upper grinding block, a partition net is installed on the inner side of the feeding groove, the top of the upper grinding block is fixedly connected with a connecting middle column, and the top end of the connecting middle column is fixedly connected with a rotating top plate. According to the biological peptide crushing device, biological peptide can be better crushed, meanwhile, by means of the special structural design, materials are repeatedly extruded and cut in the grinding process, the crushing effect is improved, and therefore the problems that biological peptide particles are high in viscosity and prone to agglomeration are effectively solved, and the biological peptide crushing device further pays attention to environmental protection and energy conservation.