Gene chip for specific detection of bacillus thuringiensis

Abstract

The invention discloses a gene chip for specific detection of bacillus thuringiensis, and belongs to a soil microorganism gene detection technology. An rpoB unique gene fragment and a 16S unique gene fragment of the bacillus thuringiensis are selected as target sequences for detection according to a reported 16S DNA sequence and rpoB gene sequence of the bacillus thuringiensis, and corresponding multiple PCR amplification primer pairs, probes and detection chips are designed. Accordingly, qualitative and quantitative detection can be conducted on the bacillus thuringiensis in a short time through the gene detection chip, and classification and determination on the bacillus thuringiensis on the species level are achieved; a powerful tool is provided for tracking colonization and reproduction of the bacillus thuringiensis in fertilizer or soil.

Description

[0001] Technical field: [0002] The invention relates to a microorganism DNA detection technology, in particular to a Bacillus thuringiensis DNA detection chip and its preparation, as well as its application in soil microorganism detection. [0003] technical background: [0004] Bacillus thuringiensis, a functional microorganism, is often used to make bio-organic fertilizers and in soil to regulate the microbial flora structure to achieve disease resistance, growth promotion, and quality improvement of crops. However, how to qualitatively and quantitatively track the colonization and reproduction behavior of this bacterium in fertilizers and soils has been perplexing relevant workers. The conventional detection of the number of effective viable bacteria has been unable to meet the needs of scientific research and production. The qualitative and quantitative analysis of common functional bacteria by gene chip technology has strong application value. At present, the traditiona...

AI Technical Summary

Problems solved by technology

At present, the traditional microbial detection method is mainly the culture method. This method has a long detection cycle and cannot classify and identify Bacillus thuringiensis, Bacillus thuringiensis, and Bacillus thuringiensis that are closely related.

In recent years, barcode sequences similar to 16S DNA have also been frequently used in the identification of microorganisms, but the difference between species of 16S DNA is very small, so it is inevitable that the classification and identification based on 16S DNA alone will have the problem of poor specificity

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