Methods and systems for acquiring nucleic acid information of parasites and classifying parasites by sequencing

Abstract

The invention relates to the field of high-throughput sequencing and particularly relates to methods and systems for acquiring nucleic acid information of parasites on a host and classifying parasites by high-throughput sequencing. The method for acquiring nucleic acid information of parasites comprises the following steps: acquiring sequence data of free DNA in host blood based on high-throughput sequencing; filtering off host nucleic acid data in the sequence data of free DNA in the host blood by taking the nucleic acid sequence of the host as a reference; filtering off repeated sequence data in the sequence data of free DNA in the host blood; and acquiring the nucleic acid data of parasites to acquire the nucleic acid information of parasites on the host. Based on the method, the invention further provides the system for acquiring the nucleic acid information of parasites, and a method and system for identifying the species of a parasite on a host. The methods and systems are not limited to prior knowledge, can improve the universality of detection methods, have high sensitivity and are rapid.

Description

technical field

[0001] The invention relates to the field of high-throughput sequencing, in particular to a method and system for obtaining nucleic acid information of parasites on a host through high-throughput sequencing, and a method and system for identifying the type of parasites on a host through high-throughput sequencing. Background technique

[0002] Parasites are a class of microorganisms that depend on their hosts for nutrients to survive. The parasites known to cause human diseases mainly include Protozoa and Helminths. There are many kinds of parasites, and different parasite infections can cause various clinical phenotypes. For different parasite infections, clinicians often need to judge the possibility of infection with a certain parasite based on their clinical phenotype assumptions, and then Do corresponding confirmatory classification and identification, such as etiological microscope classification and identification, immunological classification and iden...

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