38 results about "Infective virus" patented technology

The invention also provides a method for rapidly detecting various viruses of melons. The method comprises the following steps of: extracting total RNA of a virus; performing reverse transcription to obtain a cDNA template; performing multiple polymerase chain reaction PCR on the template by using primers; detecting the product; and determining the type of the virus, wherein 5 pairs of primers from SEQ ID No. 1 to 10 are applied in the multiple PCR. The method has the advantages of saving the time, reducing the cost and improving the efficiency during the detection of the complex infective virus of the melons.

A persistently infective virus vector is produced by using a gene so modified as to encode an amino acid sequence including a valine substituted for an amino acid residue at position-1618 in the amino acid sequence for an L protein of a persistently non-infective Sendai virus. A non-transmissible, persistently infective virus vector is also produced by defecting or deleting at least one of M gene, F gene, and HN gene. These virus vectors have no cytotoxicity, can achieve the sustained gene expression over a long period of time, is safe, and is therefore useful.

The invention relates to a sterilizing anti-viral odor-removing reusable medical photocatalyst protective mask. The sterilizing anti-viral odor-removing reusable medical photocatalyst protective mask is characterized in that an supercritical nano-scale photocatalyst coating is arranged on the outer surface of a non-woven fabric or cotton yarn fabric layer, and frameworks are arranged at the upper end and the lower end of the non-woven fabric or cotton yarn fabric layer of the photocatalyst mask. A spraying method of a photocatalyst solution comprises the steps that during spraying, the photocatalyst solution is shaken evenly and contained in a bottle, the amount of conveyed air is adjusted, the distance between a spray gun head and an object to be coated with the photocatalyst solution is 10 cm during spraying, the spraying movement speed is 0.1 m / s, the sprayed solution moves at a constant speed from left to right after being jetted, and the moving distance is 1 m; after the sprayed solution reaches the right side, a gas spraying state is adjusted, the spray gun is moved downards by 50 mm, the travel of the spray gun is adjusted to be the maximum, and then reciprocation is conducted in this way. The sterilizing anti-viral odor-removing reusable medical photocatalyst protective mask can effectively kill germs and viruses, remove odor, prevent a pollen allergy, a dust allergy and an air conditioner allergy and protect public health security and the respiratory system, has extremely powerful killing and restraining functions on infective viruses, and is disposable and reusable.

In this application is described the complete genomic sequence of avian parmyxovirus type 2, strains Yucaipa, England, Kenya and Bangor. The sequences are useful for production of recombinant infective virus, a virus vector, for vaccine development and for therapeutic compositions.

The invention relates to a detection kit and method for prawn infective virus, belonging to the technical field of marine organism pathogeny detection. The invention aims to provide a detection kit and method. The detection method is more sensitive and specific, and can shorten the reaction time and reduce the experimental steps. The detection kit comprises (1) an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) buffer tube, (2) a primer tube, (3) an enzyme tube, (4) a deionized water tube, (5) a negative control tube and (6) a positive control tube, wherein the RT-PCR buffer tube is filled with a 2*RT-PCR buffer; the primer tube is filled with infective muscle necrosis virus forward / reverse primers; the enzyme tube is filled with reverse transcriptase and DNA polymerase; the deionized water tube is filled with sterile deionized water; the negative control tube is filled with SPF (Specific Pathogen Free) prawn nucleic acid; and the positive control tube is filled with infective muscle necrosis virus cDNA (complementary deoxyribonucleic acid) positive clone. The detection method comprises (1) an RT-PCR reaction system and (2) an RT-PCR reaction procedure.

The invention is suitable for the technical field of tracking, and provides a target tracking method, a target tracking device, a storage medium and intelligent equipment. The target tracking method comprises the steps of: acquiring an MAC address of mobile equipment in a specified region; determining target mobile equipment according to the preset MAC address list and a preset MAC address list, wherein the preset MAC address list comprises a target MAC address corresponding to mobile equipment to be tracked, and the target mobile equipment is mobile equipment corresponding to an MAC address identical to the target MAC address in the preset MAC address list; acquiring first signal intensity of the target mobile equipment; determining position information of the target mobile equipment based on the first signal intensity; and tracking the target mobile equipment according to the position information of the target mobile equipment. According to the target tracking method, the tracking efficiency can be improved, and the position of a person who is diagnosed or suspected to carry infectious viruses can be tracked as soon as possible, thereby effectively reducing the possibility of cross infection of the person to others.

Cloned, i.e. defined, defective interfering (DI) influenza A virus is produced in embryonated hens eggs using a method which generates large quantities of DI virus material. Cloned DI virus is then used in tests on mice and ferrets given a lethal challenge of wild-type influenza A virus. When cloned DI influenza A virus is co-administered with a lethal dose of virulent influenza A virus, mice are protected compared to a control of inactivated cloned DI influenza A virus. Mice which survived the administration of cloned DI influenza A virus and infective challenge virus are three weeks later still protected against lethal challenge with infective virus. Control mice which received only cloned DI influenza A virus and no lethal challenge are not protected three weeks later on lethal challenge with infective virus.

Unlike the existing powered air-purifying respirators (PAPR) that are bulky, heavy, and expensive the invention offers a light-weighted inexpensive portable PAPR consisted of a single piece secured on user's head. The respirator is for use in contaminated environment including one with highly contagious viruses such as SARS-CoV-2 that causes COVID-19. A clear visor of the respirator protects eyes, nose, and mouth. The rest of the user's head protected with a soft airtight cover. A small fan or fans pump air through the filters. Because the fan capacity exceeds airflow required for breathing, air pressure under the cover is slightly higher than ambient pressure. It protects the visor and user glasses from fogging and prevents leaks through any looseness. A special port allows use of equipment and devices as a stethoscope for medics or oxygen supply for patients. Despite the respirator primarily targeted for medical personnel and patients, its convenience, l lightness and affordability allow usage by general population especially at the time of an epidemic or a pandemic.

The invention relates to a gene chip for detecting pathogen of equine infectious virus. The gene chip comprises a solid phase carrier and an oligonucleotide probe fixed on the carrier, wherein the oligonucleotide probe comprises a detection probe and a quality control probe; and the detection probe is a nucleic acid fragment selected from the nucleotide sequences of rabies virus, equine infectious arteritis virus, equine infectious rhinopneumonitis virus and equine infectious equine infectious anemia virus. The invention also relates to a method for preparing the gene chip and a method for detecting four equine infectious diseases viruses by means of the chip, comprising the steps of probe synthesis, chip preparation and hybridization with a treated and labeled to-be-detected sample. The invention further relates to a reagent kit which is used for detecting pathogen of equine infectious virus and consists of the chip, a sample treatment reagent, a hybridization reagent, a colour-producing reagent and a specification. The gene chip can detect four viruses at the same time, and has the advantages of simple operation, high accuracy and strong repeatability, and the gene chip is of great significance to both laboratory research and production practice.

In this application is described the complete genomic sequence of avian parmyxovirus type 2, strains Yucaipa, England, Kenya and Bangor. The sequences are useful for production of recombinant infective virus, a virus vector, for vaccine development and for therapeutic compositions.

The invention relates to a protein and virus quickly-enriching method, composed of the steps: 1), flocculating protein: diluting the sample to release protein and virus particles, and adding in agglomerant to make protein and virus particles form agglutinating matter; 2), making separate-phase deposition: adding in phase-separating agent to deposit protein and virus particles and cells between phases; 3), making secondary deposition: adding in depositing solvent to deposit them to the tube bottom; 4), eliminating clear solution and retaining deposit: eliminating top clear solution, washing, centrifuging, re-eliminating top clear solution, re-centrifuging, and then absorbing all the top clear solution to get the enriched solution. It can be finished within 10-20 minutes. It can concentrate by about 100 times, making detection rate and accuracy of protein and virus raised by 100-1000 times. Its enriching speed is specially fast.

The present invention relates to a noninfectious virus recombinant gene SARS-CoV-EGFP for screening medicine for resisting SARS virus and making basic research of virology. In 5' end of total genome of SARS coronate virus the promotor sequence T7 Pro. is introduced, the coding sequence of external structural domain related to viral pathogenicity and toxicity in S protein can be sustituted with EGFD gene of code enhanced green fluorescin, and in the 3' end of total genome HDV antisense genome ribozyme sequence HDV R2 and T7 transcription terminator sequence T7 Ter are successively introduced to create recombinant gene SARS-CoV-EGFP, then it is inserted into carrier to construct recombinant plasmid pUC19 / SARS-CoV-EGFP, after cell transfection, through replication, transcription, traslation, processing and packaging to obtain recombinant virus capable of making transfected cell give out green fluorescence under the fluorescent microscope.

The invention discloses a method and a device for detecting and clearing an infective virus in a PE (portable executable) file. The method comprises the following steps of judging whether an PE file head of a target PE file contains a preset feature which is suspected to infect with the infective virus or not; when the PE file head of the target PE file contains the preset feature, preprocessing the target PE file; executing the corresponding file in a debug type, and setting an int3 breakpoint at an entrance point of the corresponding file; when the file is executed to the entrance point, if an invalid API (application program interface) call is detected, removing the call; processing a debug event, and finishing the executing process until the abnormal condition occurs and the debug event cannot be processed; matching the content of a final valid byte in the PE structure of the target PE file in a current memory and the virus feature of a preset virus base; according to the matching result, determining whether the target PE file is infected with the infectious virus or not, and clearing the virus of the target PE file infected with the infective virus. The method has the advantage the virus defection efficiency is improved.

The invention discloses a compound radix bupleuri and analgin transdermal agent and a preparation method thereof. The compound radix bupleuri and analgin transdermal agent is prepared from the whole grass of radix bupleuri grass, analgin, sodium thiosulfate, EDTA-2Na, azone, Tween 80 and dimethyl sulfoxide by certain weight proportion. The invention has the characteristics that the administration is realized by spraying through the respiratory system or the secondary cuticula of the ears, the treatment of the diseases of livestock and poultry is quick, simple and convenient, the treatment cost is reduced, the cost of the medicine is lowered, the medicine effect is lasting, and the use range is extensive. The invention is widely used for treating the diseases of the cold, the pyrexia, the fever, the blue ear and the like of the livestock and the poultry caused by the infective viruses of the respiratory tract and the digestive tract.

The invention provides an enzyme-catalyzed conductivity immune sensor based on a micro-gap array electrode and a method which is used for detecting infectious virus, and the sensor comprises a micro-gap array electrode, an electrode substrate which is carried out the silanization treatment and enzyme sediments with great conductivity. The invention utilizes the micro-gap electrode array immune sensor to carry out the detection of human immunodeficiency virus (HIV) antibody and hepatitis B virus surface antigen (HBsAg), thus providing the diagnosis method of infectious diseases with rapidity, high sensitivity and high throughput and possibly providing the effective technical means for the early diagnosis and the on-site screening of the infectious diseases.

This invention relates to a method for recombinantly producing via rescue canine distemper virus, a nonsegmented, negative-sense, single-stranded RNA virus, and immunogenic compositions formed therefrom. Additional embodiments relate to methods of producing the canine distemper virus as an attenuated and / or infectious viruses. The recombinant viruses can be prepared from cDNA clones, and, accordingly, viruses having defined changes, including nucleotide / polynucleotide deletions, insertions, substitutions and rearrangements, in the genome can be obtained.

The invention provides a method and a device for detecting infective viruses, wherein the method comprises the following steps of extracting a feature vector, which comprises structural characteristics of susceptible type virus infection, of a to-be-detected file; detecting whether the to-be-detected file is an infective virus file or not according to the feature vector by utilizing an infective virus identifying model which is obtained by performing machine learning based on the feature vector. The method overcomes the problem that the labor cost for manually analyzing, identifying and starting rules is high; moreover, the method for detecting infective viruses based on the infective virus identifying model greatly increases the detecting speed and can effectively detect unknown infective viruses.

Cloned, i.e. defined, defective interfering (DI) influenza A virus is produced in embryonated hens eggs using a method which generates large quantities of DI virus material. Cloned DI virus is then used in tests on mice and ferrets given a lethal challange of wild-type influenza A virus. When cloned DI influenza A virus is co-administered with a lethal dose of virulent influenza A virus, mice areprotected compared to a control of inactivated cloned DI influenza A virus. Mice which survived the administration of cloned DI influenza A virus and infective challange virus are three weeks later still protected against lethal challange with infective virus. Control mice which received only cloned DI influenza A virus and no lethal challange are not protected three weeks later on lethal challange with infective virus. A therapeutic benefit of administering cloned DI influenza a virus is found when the administration takes place in less than 48 hours after challange with infective virus. Cloned DI influenza A virus of one subtype is found to act in vivo as an effective antiviral against the same or any other sub-type of influenza A virus. The antiviral effect has been found to have both a therapeutic and a prophylactic application against influenza A infection in humans, mammals and birds.

The invention relates to a method for establishing an HCV (hepatitis C virus) cell model by utilizing tree shrew bone marrow mesenchymal stem cells, and belongs to the technical field of biological medicines. The method comprises the steps that firstly, an OCLN (occludin) lentiviral vector is adopted to infect the tree shrew bone marrow mesenchymal stem cells, then a CD81 lentiviral vector is added for further infection, then a miR-122 lentiviral vector is added for infection for 4 hours, then a fresh culture medium containing 4 mcg / mL of polybrene with 1 / 2 volume of a current culture medium is added, and the used culture medium is removed the next day and is replaced with the fresh culture medium for continuous culture so as to obtain the HCV (hepatitis C virus) cell model. According to the method, the cell model has the basic characteristics and the differentiation potential of the bone marrow mesenchymal stem cells and is susceptible to HCV (hepatitis C virus), HCV (hepatitis C virus) endocytosis and copy are supported, infective virus particles are produced, and new cell model resources are provided for HCV (hepatitis c virus) pathogenesis researching and drug screening.

The invention provides a method and a device used for detecting infective viruses. The method comprises the following steps: extracting the feature vector of a file to be detected, wherein the feature vector comprises the distribution frequency of an entropy of the immediate data of an entry point; and utilizing an infective virus identification model obtained by machine learning on the basis of the feature vector to detect whether the file to be detected is an infective virus file or not on the basis of the feature vector. The problems of high manual cost of manual analysis identification and manual rule startup are overcome, and the method for detecting the infective viruses on the basis of the infective virus identification model greatly improves detection speed, and can effectively detect unknown infective viruses.

A persistently infective virus vector is produced by using a gene so modified as to encode an amino acid sequence including a valine substituted for an amino acid residue at position-1618 in the amino acid sequence for an L protein of a persistently non-infective Sendai virus. A non-transmissible, persistently infective virus vector is also produced by defecting or deleting at least one of M gene, F gene, and HN gene. These virus vectors have no cytotoxicity, can achieve the sustained gene expression over a long period of time, is safe, and is therefore useful.

The invention relates to an atomized traditional Chinese medicine gas dust filtration and virus isolation inactivation respirator, belongs to the field of environmental protection, and aims to solve the technical problem that virus can be spread again due to the fact that a traditional mask does not have a disinfection function. The specific method for solving the problem is characterized in thatthe respirator consists of a rigid chassis support body 1, a rotating shaft 2, an atomized traditional Chinese medicine box 3, a negative pressure gas dust filtration and virus inactivation box 4, a positive pressure gas virus inactivation box 5, a gas guide pipe 6, a flat grid bar 7, an atomized flat grid bar 8, an inactivation flat grid bar 9, a sealing ring 10, a sealing cover 11, a negative pressure exhaust pipe 12, a negative pressure air inlet pipe 13, a positive pressure air outlet 14, a positive pressure air inlet pipe 15 and a strap hanging column 16. The respirator is mainly used forpreventing infectivity virus from spreading again. The respirator adopts a technical method for inactivating virus and filtering dust and atomizing traditional Chinese medicine through one-way airflow, and the purposes of negative pressure supply of clean traditional Chinese medicine atomized gas, positive pressure inactivation of ambient gas virus and killing of virus in the lung of an inhaler are achieved.

The present invention relates to a noninfectious virus recombinant gene SARS-CoV-EGFP for screening medicine for resisting SARS virus and making basic research of virology. In 5' end of total genome of SARS coronate virus the promotor sequence T7 Pro. is introduced, the coding sequence of external structural domain related to viral pathogenicity and toxicity in S protein can be sustituted with EGFD gene of code enhanced green fluorescin, and in the 3' end of total genome HDV antisense genome ribozyme sequence HDV R2 and T7 transcription terminator sequence T7 Ter are successively introduced to create recombinant gene SARS-CoV-EGFP, then it is inserted into carrier to construct recombinant plasmid pUC19 / SARS-CoV-EGFP, after cell transfection, through replication, transcription, traslation, processing and packaging to obtain recombinant virus capable of making transfected cell give out green fluorescence under the fluorescent microscope.

Cloned, i.e. defined, defective interfering (DI) influenza A virus is produced in embryonated hens eggs using a method which generates large quantities of DI virus material. Cloned DI virus is then used in tests on mice and ferrets given a lethal challenge of wild-type influenza A virus. When cloned DI influenza A virus is co-administered with a lethal dose of virulent influenza A virus, mice are protected compared to a control of inactivated cloned DI influenza A virus. Mice which survived the administration of cloned DI influenza A virus and infective challenge virus are three weeks later still protected against lethal challenge with infective virus. Control mice which received only cloned DI influenza A virus and no lethal challenge are not protected three weeks later on lethal challenge with infective virus.

The present invention discloses a human papilloma virus cerevisiae proliferation model and a preparation method, which is the saccharomyces cerevisiae cell Y303 ATCC No.96659 or saccharomyces cerevisiae cell YPH500 ATCC No.76626 of a HPV genome containing a cerevisiae nutrition defect screening mark Ura3. The model of the present invention can be used for researching on the function of HPV gene, or a cerevisiae RNA interfere technology is used for researching on the functions of different genes, or a cerevisiae expression vector is introduced again to express interested foreign protein in cells, so as to research on the effect of the cerevisiae expression vector in virus living period, or virus main capsid protein L1 expression vector is introduced for packing infective virus granules to be used for the experiment or research on HPV bacterin.

The invention relates to a method for detecting cytotoxicity of an anti-HCV medicament. 2a-type chimeric virus J6 / JFH1 is utilized to transfect Huh7.5.1 cell to generate an HCV cell culture system (HCV cell culture, HCVcc) with infective virus particles, an MTT method for analyzing cell activity and cell growth and proliferation is utilized to detect the cytotoxicity of the HCV medicament, the inhibition ratio of the medicament to cell growth is calculated, and IC50 is obtained by calculation through Graphpad Prism 5. The method has the advantage of simple operation, economy, rapidness, sensitivity and no radioactive pollution, can accurately detect the cytotoxicity of the medicament, has good repeatability, and is an accurate detection method for smoothly implementing evaluation of in-vitro antiviral medicinal effect.

The invention also provides a method for rapidly detecting various viruses of melons. The method comprises the following steps of: extracting total RNA of a virus; performing reverse transcription to obtain a cDNA template; performing multiple polymerase chain reaction PCR on the template by using primers; detecting the product; and determining the type of the virus, wherein 5 pairs of primers from SEQ ID No. 1 to 10 are applied in the multiple PCR. The method has the advantages of saving the time, reducing the cost and improving the efficiency during the detection of the complex infective virus of the melons.

The invention relates to a method for detecting cytotoxicity of an anti-HCV medicament. 2a-type chimeric virus J6 / JFH1 is utilized to transfect Huh7.5.1 cell to generate an HCV cell culture system (HCV cell culture, HCVcc) with infective virus particles, an MTT method for analyzing cell activity and cell growth and proliferation is utilized to detect the cytotoxicity of the HCV medicament, the inhibition ratio of the medicament to cell growth is calculated, and IC50 is obtained by calculation through Graphpad Prism 5. The method has the advantage of simple operation, economy, rapidness, sensitivity and no radioactive pollution, can accurately detect the cytotoxicity of the medicament, has good repeatability, and is an accurate detection method for smoothly implementing evaluation of in-vitro antiviral medicinal effect.

The invention provides a method and a device for detecting infective viruses. The method comprises the following steps: extracting a feature vector of a file to be detected, wherein the feature vector comprises the instruction frequency of a code of an entry point; and utilizing an infective virus identification model obtained by machine learning on the basis of the feature vector to detect whether the file to be detected is an infective virus file or not. The problems of high manual cost of manual analysis identification and manual rule startup are overcome, and the method for detecting the infective viruses on the basis of the infective virus identification model greatly improves detection speed, and can effectively detect unknown infective viruses.