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Bifidobacterium longum subsp. Longum capable of preventing and relieving colitis symptoms and application thereof

A technology for Bifidobacterium longum and colitis, applied in the field of microorganisms, can solve the problems of increasing the probability of patients suffering from intestinal cancer, reducing the quality of life of patients, and not being able to use them in large quantities for a long time, so as to improve stool properties and blood in the stool, and reduce mRNA expression. , Improve the effect of intestinal flora changes

Active Publication Date: 2022-03-11
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

UC has a long course of disease and is prone to recurrence. While reducing the quality of life of patients, it also increases the chance of patients suffering from bowel cancer
[0004]With the increasing incidence of UC in China, commonly used drugs such as aminosalicylic acid, hormones and immunosuppressants for the treatment of UC have large side effects and are expensive, although temporary Effective in controlling disease activity, but cannot be used in large quantities over a long period of time

Method used

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  • Bifidobacterium longum subsp. Longum capable of preventing and relieving colitis symptoms and application thereof
  • Bifidobacterium longum subsp. Longum capable of preventing and relieving colitis symptoms and application thereof
  • Bifidobacterium longum subsp. Longum capable of preventing and relieving colitis symptoms and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: Screening, identification, cultivation, observation and preservation of Bifidobacterium longum subspecies KLDS K5

[0039] 1. Screening

[0040]Take 1g of feces samples from healthy infants in Harbin, spread them on MRS solid medium (including rapid screening) after serial dilution, and culture them in an anaerobic environment at 37°C for 72 hours, observe and record the colony morphology; , Protruding, white and yellowish colonies were streaked on the MRS solid medium, and purified and cultivated under anaerobic conditions at 37°C. Repeat this operation 3 times to obtain purified single colonies; pick a single colony on Streak on the MRS solid medium and incubate anaerobically at 37°C for 36h.

[0041] 2. Identification

[0042] Extract the genome of the bacterial strain obtained by screening, amplify and sequence the 16S rDNA of the bacterial strain (the nucleotide sequence of the 16S rDNA obtained by amplification is shown in SEQ ID NO.1), and carry o...

Embodiment 2

[0048] Protective effect of Bifidobacterium longum subsp. longum KLDS K5 on colon tissue of DSS-induced colitis mice:

[0049] (1) Preparation of 3% DSS solution: dextran sodium sulfate (DSS) was prepared into a 3% (w / v) DSS solution with sterilized tap water.

[0050] (2) 40 C57BL / 6J mice (SPF grade, male, 7 weeks old, 22-24g) were provided by Beijing Weishenghe Experimental Animal Technology Co., Ltd. All mice were acclimatized for 7 days before the experiment. Mice were reared in cages under standard temperature (20°C±2°C), humidity (50%±5%), and photoperiod (12h / 12h light / dark cycle) conditions of Northeast Agricultural University (Harbin). The experimental period is 22 days

[0051] (3) All mice were randomly divided into 4 groups, 10 in each group, which were blank control group, model group (DSS), long subspecies KLDS K5 intervention group and BB-12 group, as shown in Table 1. All mice were free to eat and drink from day -6 to day 0. Mice in the long subspecies KLDS...

Embodiment 3

[0067] Protective effect of Bifidobacterium longum subsp. longum KLDS K5 on the colonic mucosal barrier in colitis mice:

[0068] The mucin on the surface of the colonic epithelial cells can be stained with AB, showing blue, and the staining results of AB in mouse colon tissue are as follows: Figure 7 As shown, the colonic mucin content of the mice in the blank group was abundant, while the colonic mucin content of the mice in the DSS group was significantly reduced, and this change was improved in the Bifidobacteria treatment group. obviously increase.

[0069] MUC2 is one of the main components of mucin, and the effects of Bifidobacterium longum subsp. longum on the mRNA expression level of MUC2 in colon tissue are as follows: Figure 8 As shown, after DSS induction, the mRNA expression level of MUC2 in the mouse colon was significantly (p<0.05) lower than that in the blank group, and all tested bifidobacteria could significantly (p<0.05) up-regulate the mRNA expression of...

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Abstract

The invention provides bifidobacterium longum subsp. Longum KLDS K5 capable of preventing and relieving symptoms of colitis, and belongs to the technical field of microorganisms. The invention further provides a preparation method of the bifidobacterium longum subsp. Longum KLDS K5. Experiments show that the bifidobacterium longum provided by the invention can tolerate the human gastrointestinal environment, relieve weight loss in the ulcerative colitis disease period, improve colonic mucosa injury and reduce MPO activity, and inhibit oxidation-related factors through iNOS and COX-2 signal pathways so as to relieve inflammatory bowel diseases; nF-kappa B p65 nuclear transfer is inhibited through a TLR4 / MyD88 / NF-kappa B signal channel, the expression quantity of proinflammatory factors TNF-alpha, IL-1beta, IL-6 and IL-10 in colon is reduced, the transcriptional level of colon tight connection related proteins Claudin-1, ZO-1 and Occludin and mucoprotein MUC2 is up-regulated, the intestinal flora change after DSS induction can be improved in the genus level, and the richness and diversity of the intestinal flora are improved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Bifidobacterium longum subsp. longum capable of preventing and alleviating colitis symptoms and its application. Background technique [0002] Inflammatory bowel disease (IBD) is a chronic, non-specific intestinal inflammatory disease that mainly accumulates in the colorectum and ileum, mainly including ulcerative colitis (UC) and Crohn's disease (Crohn's disease). disease, CD), the clinical manifestations are abdominal pain, diarrhea, mucus pus and blood in the stool. At present, its pathogenesis is not completely clear, and it tends to be that genetic, diet, environmental and other factors act on genetically susceptible hosts to trigger abnormal immune responses and lead to chronic intestinal inflammation. [0003] Ulcerative colitis (UC) is a form of IBD with unknown etiology. It mainly manifests as non-specific inflammation of the rectum and colon, with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/745A61P1/00C12R1/01
CPCC12N1/20A61K35/745A61P1/00A61K2300/00
Inventor 霍贵成赵莉李柏良董佳欢刘德玉徐宝峰刘飞王松
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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