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Prebiotic composition and application thereof in inhibiting pathogenic bacterium adhesion and improving intestinal epithelial cell barrier

A technology of composition and prebiotics, which is applied in the field of compositions regulating human intestinal microecology, can solve the problems of unrecorded oligosaccharide mixture, lack of oligosaccharide research, and no anti-adhesion effect, etc., to achieve inhibition of intestinal barrier Effects of damage, inhibition of adhesion, and enhancement of barrier function

Pending Publication Date: 2022-07-29
量子高科(广东)生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the current research on functional oligosaccharides, it is found that some oligosaccharides have no anti-adhesion effect on pathogenic bacteria. Among the functional sugars that have been found to have anti-adhesion effects, the mixture of these oligosaccharides is mostly used as the research object. There is no research on single-component oligosaccharide structure as the research object, and it has not been recorded whether the existing oligosaccharide mixture has the effect of improving the intestinal epithelial cell barrier
[0004] In addition, there is a lack of research on some functional oligosaccharides that are difficult to produce domestically, such as 2'-fucosyllactose (2'-FL), 3-fucosyllactose (3-FL) in human milk oligosaccharides. FL), it has been documented that it has the ability to resist Staphylococcus aureus infection, but whether it has inhibitory effect on other pathogenic bacteria and whether it can improve the barrier function of intestinal epithelial cells is unknown

Method used

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  • Prebiotic composition and application thereof in inhibiting pathogenic bacterium adhesion and improving intestinal epithelial cell barrier
  • Prebiotic composition and application thereof in inhibiting pathogenic bacterium adhesion and improving intestinal epithelial cell barrier
  • Prebiotic composition and application thereof in inhibiting pathogenic bacterium adhesion and improving intestinal epithelial cell barrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] This embodiment provides a prebiotic composition. In parts by weight, the prebiotic composition includes 5-30 parts of 2'fucosyllactose, 0-20 parts of 3-fucosyllactose, and 10 parts of fructooligosaccharides. ~100 parts and 10-100 parts of galactooligosaccharide, the specific proportion of each component is shown in Table 1. The fructooligosaccharide used in this example is FOS-GF 2 -80, galactooligosaccharide is GOS90.

[0033] effect test

[0034] (1) Effect test of prebiotic composition on inhibiting the adhesion of Escherichia coli to Caco-2 cells

[0035] Each sample described in Table 1 was subjected to the anti-adhesion effect test of pathogenic bacteria Escherichia coli K88, and the adhesion inhibition rate of each prebiotic composition sample against the adhesion of Escherichia coli K88 was counted, and the specific test method was as follows:

[0036] Caco-2 cells were cultured in DMEM complete medium, 5% CO 2 . The cells were cultured in a 37°C incubator ...

Embodiment 2

[0050] In this example, the inhibitory effect of the prebiotic composition of the present invention on pathogenic bacteria is analyzed by comparing with other component oligosaccharides, and the specific test method is as follows:

[0051] (1) Culture medium treatment

[0052] LB broth medium (without sugar) components: tryptone 10.0g / L, yeast extract 5.0g / L, sodium chloride 5.0g / L, final pH 7.0±0.2 (25°C). Weigh 20 g of sugar-free LB broth medium, add 1000 mL of distilled water or deionized water, stir and heat to boil until completely dissolved, divide into conical flasks, sterilize by autoclaving at 121 °C for 15 min, and set aside.

[0053] 20g / L of the following compositions were added to the LB broth medium without sugar:

[0054] A. Lactose+FOS-GF 2 -80+GOS90 (weight ratio 30:30:40);

[0055] B. 2'-FL+lactose+FOS-GF 2 -80+GOS90 (weight ratio 15:20:30:35);

[0056] C. 2'-FL+3-FL+inulin+GOS90 (weight ratio 10:10:40:40);

[0057] D. 2'-FL+3-FL+FOS-GF 2 -50+GOS90 (we...

Embodiment 3

[0073] In this example, each component in the prebiotic composition is adjusted to analyze the inhibitory effect of each component on Escherichia coli.

[0074] With reference to the test method for the effect of the prebiotic composition on inhibiting the adhesion of Escherichia coli to Caco-2 cells described in Example 1, each prebiotic in Table 2 was used as the test object, and the results of the adhesion inhibition rate of each prebiotic sample to Escherichia coli K88 were analyzed. , the results are shown in Table 2. The results show that the inhibitory effects of different components on E. coli K88 are slightly different, and the reason for the different differences may be the influence of components, and the interaction between different components may be It also affects the suppression effect. Overall, the inhibitory effect of sample 1 on Escherichia coli K88 is the most obvious, followed by sample 14 and sample 15. Compared with the prebiotic composition formed by a...

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Abstract

The invention belongs to the field of compositions for regulating human intestinal microecology, and particularly relates to a prebiotic composition and application thereof in inhibiting pathogenic bacterium adhesion and improving intestinal epithelial cell barrier. The prebiotic composition comprises 5 to 30 parts of 2 '-fucosyllactose, 0 to 20 parts of 3-fucosyllactose, 10 to 100 parts of fructo-oligosaccharide and 10 to 100 parts of galactooligosaccharide, and the prebiotic composition formed by compounding 2'-FL, 3-FL, FOS and GOS according to a specific ratio not only can effectively inhibit the adhesion of Escherichia coli, but also can inhibit the growth of the Escherichia coli. And by promoting expression of intestinal epithelial cell tight junction proteins ZO-1, Occludin and Claudin-1, the barrier function of the intestinal tract is directly enhanced, and the intestinal tract barrier injury induced by pathogens is inhibited.

Description

technical field [0001] The invention belongs to the field of compositions for regulating human intestinal microecology, in particular to a prebiotic composition and its application in inhibiting the adhesion of pathogenic bacteria and improving the barrier of intestinal epithelial cells. Background technique [0002] Except for infections caused by insect bites or trauma that direct pathogens into tissues or the bloodstream, the vast majority of infections start from the adhesion of pathogens to the surface of host mucosal epithelial cells, which is an essential first step in the formation of infection. When pathogenic bacteria firmly adhere to the surface of susceptible tissue, some grow and multiply on the surface of the tissue, causing disease; some enter the cell to grow and multiply, producing toxic substances and causing cell death, resulting in superficial tissue damage. Ulcers; some pathogens spread further through the mucosal epithelial cells or intercellular substa...

Claims

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Application Information

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IPC IPC(8): A61K31/702A61P1/00A61P31/04A61K31/7016
CPCA61K31/702A61K31/7016A61P1/00A61P31/04A61K2300/00
Inventor 王炜宏杨新球陈子健王涛郝占西
Owner 量子高科(广东)生物有限公司
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