Application of lactobacillus reuteri in preventing and relieving ulcerative colitis
A technology of Lactobacillus reuteri and ulcerative colitis, applied in the field of functional microorganisms, to achieve the effect of improving colonic mucosal damage, reducing relative abundance, and good tolerance
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Embodiment 1
[0053] Example 1: Lactobacillus reuteri CCFM1135 has good tolerance to simulated gastrointestinal fluid
[0054] Inoculate cryopreserved Lactobacillus reuteri CCFM1135 in mMRS medium, culture anaerobically at 37°C for 48 hours, and subculture 2 to 3 times in mMRS medium, take 1 mL of Lactobacillus reuteri CCFM1135 culture medium , mixed with 9.0mL pH 2.5 artificial simulated gastric juice (mMRS medium containing 1% pepsin, pH = 2.5), and anaerobically cultured at 37°C, samples were taken at 0h, 0.5h, 1h, 2h and 3h, and used The mMRS agar medium was poured and cultured for plate colony counting, the number of viable bacteria was determined and the survival rate was calculated (see Table 1).
[0055] Take 1 mL of the culture solution of Lactobacillus reuteri CCFM1135 and add it to 9 mL of artificial simulated intestinal fluid (mMRS medium containing 0.3% bovine bile salt, 1% trypsin, pH=8.0), and culture it anaerobically at 37°C. , 0.5h, 1h, 2h, 3h and 4h, take samples, use mMR...
Embodiment 2
[0061] Example 2: Lactobacillus reuteri CCFM1135 has no toxic side effects on C57BL / 6J mice
[0062] Resuspend Lactobacillus reuteri CCFM1135 in 3mg / 100mL (w / v) sucrose solution to make a concentration of 5.0×10 9 CFU / mL bacterial suspension. Take 6 healthy male C57BL / 6J mice with a body weight of about 14-16g. After adapting to the environment for a week, they are given the bacterial suspension of this concentration by gavage once a day (0.2mL per gavage), observed for a week, and recorded the death and body weight. .
[0063] The results of these tests are listed in Table 3. These results indicated that feeding concentrations of 5.0×10 9 CFU / mL of Lactobacillus reuteri CCFM1135 had no significant impact on mice, no significant change in body weight, and no death. The appearance of the mice had no obvious pathological symptoms.
[0064] Table 3 Changes in body weight and death of mice
[0065] time (days) 1 2 3 4 5 6 7 weight(g) 20.13±0.42 20.42±...
Embodiment 3
[0067] Example 3: Effect of Lactobacillus reuteri CCFM1135 on disease symptoms in UC mice
[0068] Take 30 healthy male C57BL / 6J mice weighing 14-16g, adapt to the environment for 1 week, 6 mice in each group, and randomly divide them into 5 groups: blank group, model group, drug group, Lactobacillus reuteri CCFM1135 intervention group (CCFM1135), Lactobacillus reuteri FCQHC8L6 control group (FCQHC8L6). The dose of intragastric bacterial suspension was 5.0×10 9 CFU / mL, resuspended in 3% (w / v) sucrose solution, orally administered 0.2 mL per day. In the fifth week, 2.5% (w / v) dextran sodium sulfate (dextransulphate sodium, DSS) was added to drinking water for 7 days to induce colitis in mice. The grouping and treatment methods of the experimental animals are shown in Table 4.
[0069] Table 4 Animal experiment design
[0070]
[0071]
[0072] In the fifth week, that is, during the modeling period (DSS treatment), the body weight of the mice was regularly weighed ever...
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