Preparation method of spray reagent for preventing avian influenza of livestock and poultry

An avian flu and drug technology, which is applied in the field of spray drug preparation, can solve problems such as liver and kidney damage, drug residues, etc., and achieve the effects of promoting healthy growth, maintaining health, and promoting recycling.

Inactive Publication Date: 2018-05-29
袁春华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention: aiming at the problem that the current existing medicines for bird flu are basically composed of chemical substances, which are easy to produce drug residues in livestock and poultry, and cause damage to the liver and kidneys of poultry when taken for a long time, a kind of medicine is provided. Preparation method of spray agent for preventing livestock and poultry avian influenza

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027] A method for preparing a spray agent for preventing influenza in livestock and poultry, the preparation method comprising the steps of:

[0028] (1) Slice the camellia root, dry it in vacuum at a temperature of 50~60°C for 20~24 hours, and crush it to obtain a crushed product. According to a mass ratio of 1:3~4, put the crushed product and petroleum ether at a temperature of 70~80°C Reflux and degrease for 1~2h, filter with suction to get the filter residue, mix the filter residue and distilled water according to the mass ratio of 1:20~30, soak for 5~6h, and then ultrasonically extract at a temperature of 40~60°C and an ultrasonic power of 600~1200W 10~50min, filter under reduced pressure to obtain the filtrate, concentrate the filtrate under reduced pressure, add ethanol 3~4 times the weight of the filtrate, sink at a temperature of 2~4°C for 20~24h, centrifuge at 3000~4000r / min for 8~ After 10 minutes, the precipitate was obtained, and the precipitate was dried at a t...

Embodiment 1

[0033] Chitosan solution The chitosan solution is mixed with chitosan and 0.2mol / L acetic acid solution at a mass ratio of 1:7, and stirred in a water bath at a temperature of 45°C at a rate of 200r / min for 6 hours to obtain a shell. polysaccharide solution.

[0034] The nutrient solution is calculated in parts by weight. Take 40 parts of water, 10 parts of peptone, 5 parts of sodium chloride, and 3 parts of beef extract powder, mix them evenly, and sterilize at high temperature to obtain the nutrient solution.

[0035] The liquid medium is calculated in parts by weight, and 70 parts of water, 20 parts of glucose, 10 parts of peptone, 10 parts of beef extract, 5 parts of yeast extract, 5 parts of sodium acetate, 2 parts of diammonium citrate, and 1 part of Tween 80. Mix 0.5 part of magnesium sulfate and 0.25 part of magnesium sulfate evenly, and sterilize at high temperature to obtain a liquid medium.

[0036] Secondary activation refers to putting it into the activation solu...

Embodiment 2

[0043] Chitosan solution Chitosan solution is prepared by mixing chitosan and 0.2mol / L acetic acid solution according to the mass ratio of 1:8.

[0044] Mix and stir in a water bath at a rate of 250r / min for 7h at a temperature of 47°C to obtain a shell

[0045] polysaccharide solution.

[0046] The nutrient solution is calculated in parts by weight. Take 45 parts of water, 12 parts of peptone, 5 parts of sodium chloride, and 4 parts of beef extract powder, mix them evenly, and sterilize at high temperature to obtain the nutrient solution.

[0047] The liquid medium is calculated in parts by weight, and 75 parts of water, 25 parts of glucose, 12 parts of peptone, 12 parts of beef extract, 5 parts of yeast extract, 5 parts of sodium acetate, 2 parts of diammonium citrate, and 1 part of Tween 80. Mix 0.7 parts of magnesium sulfate and 0.3 parts of magnesium sulfate evenly, and sterilize at high temperature to obtain a liquid culture medium.

[0048] Secondary activation refers...

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PUM

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Abstract

The invention belongs to the technical field of animal drugs and particularly relates to a preparation method of a spray reagent for preventing avian influenza of livestock and poultry. According to the spray reagent disclosed by the invention, a Chinese herbal probiotic preparation can protect respiratory mucosa and can be also used as a disinfectant for improving the environment of the livestockand poultry; the aim of disinfecting is achieved by using an antagonistic action of probiotics and metabolite thereof to pathogenic bacteria and conditioned pathogen. Chinese herbal medicines and theprobiotics have a synergistic effect, and further the capability of diffusion propagation of the avian influenza is weakened; in addition, the avian influenza is prevented and treated by a spray mode, so that the damage to liver and kidney caused by long-term administration is avoided.

Description

technical field [0001] The invention belongs to the technical field of animal medicines, and in particular relates to a preparation method of a spray medicine for preventing livestock and poultry avian influenza. Background technique [0002] Avian influenza is the abbreviation of avian influenza. It is an infectious disease of poultry caused by influenza A virus. It mainly occurs in chickens, turkeys, guinea birds and other birds, especially migratory waterfowl such as wild ducks and swans. According to the different types of pathogens, avian influenza can be divided into three categories: highly pathogenic, low pathogenic and non-pathogenic avian influenza: non-pathogenic avian influenza does not cause obvious symptoms, and only causes infection in the body of infected birds. produce antiviral body; low pathogenic avian influenza can cause mild respiratory symptoms, reduced food intake, decreased egg production, and sporadic death in poultry; highly pathogenic avian influe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/539A61K9/12A61K47/36A61P31/16A61P37/04A61L2/22A61K35/742A61K35/747A61K35/744A61L101/56A61L101/52
CPCA61K36/539A61K9/12A61K35/742A61K35/744A61K35/747A61K36/282A61K36/284A61K47/36A61K2236/331A61K2236/39A61K2236/51A61K2236/53A61L2/22A61L2202/25A61K2300/00
Inventor 袁春华乔舍林凡
Owner 袁春华
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