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Composition based on brown algae extract and lactobacillus reuteri and application of composition

A technology of Lactobacillus reuteri and composition, which is applied in the field of composition based on brown algae extract and Lactobacillus reuteri, can solve the problems of slow action and effect not reaching the expected effect, and increase insulin secretion , Improve the colonization rate, reduce the effect of fasting blood sugar and glycosylated hemoglobin concentration

Active Publication Date: 2021-08-06
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when Lactobacillus reuteri is used or mixed with other lactic acid bacteria to directly regulate the intestinal tract, the effect is slow, and the effect is often not as expected.

Method used

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  • Composition based on brown algae extract and lactobacillus reuteri and application of composition
  • Composition based on brown algae extract and lactobacillus reuteri and application of composition
  • Composition based on brown algae extract and lactobacillus reuteri and application of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Effect of brown algae extract on the growth of Lactobacillus reuteri

[0033] Preparation of brown algae extract: Take 10g (dry weight) of kelp, add 1L of deionized water, beat and break, add alginate lyase enzyme solution according to the enzyme-to-bottom ratio of 5000U / g, stir at room temperature for 8 hours, and then boil the water bath to make the enzyme Inactivation, the water extract is lyophilized after ultrafiltration to remove enzymes, and the obtained brown algae extract is obtained.

[0034] In the ultra-clean workbench, the bacterial solution in the preservation tube was dipped, and after the plate was streaked, it was cultured overnight at 37°C in an anaerobic workstation. Pick a single colony on the plate to inoculate and activate in liquid medium for 24 hours, and then carry out fermentation culture according to the inoculum amount of 5.0%.

[0035] Lactobacillus reuteri fermentation liquid was inoculated into the culture medium with different...

Embodiment 2

[0036] Embodiment 2: Brown algae extract promotes Lactobacillus reuteri to produce short-chain fatty acids

[0037] Centrifuge the fermentation broth samples of Lactobacillus reuteri in different additive media at 12000r / min for 5min, filter the supernatant with a 0.22μm filter membrane, and detect short-chain fatty acids by HPLC. Chromatographic column: Waters XBridge C18 5μm, mobile phase : 30% acetonitrile, 70% water (0.05% phosphoric acid), column temperature: 35°C, flow rate: 0.5mL / min, injection volume: 20μL.

[0038] Lactobacillus reuteri hardly produces short-chain fatty acids in the medium without additives, and the total acid content is less than 10mg / mL; after adding brown algae extract and mannan oligosaccharides, the production of short-chain fatty acids is significantly increased, mainly concentrated in acetic acid , butyric, propionic and valeric acids, especially butyric, valeric and isovaleric acids. Among them, butyric acid is the most important short-chain ...

Embodiment 3

[0039] Embodiment 3: Brown algae extract promotes Lactobacillus reuteri to form biofilm

[0040] Lactobacillus reuteri was formulated into a bacterial concentration of 1.0×10 7 cfu / mL bacterial suspension, placed in a 96-well plate for 24 hours, discarded the culture medium, added 0.2mL 1% crystal violet for 30min, rinsed with distilled water and added 0.2mL 95% ethanol, the resulting colored solution had a wavelength of 590nm Measure the OD as the OD of Lactobacillus reuteri in the medium without oligosaccharides 590nm as comparison. The method for judging the ability of bacteria to form biofilm is: OD≤Dc, no biofilm formation ability; Dc4Dc, strong biofilm formation ability ability to form.

[0041] Comparing the staining results, Lactobacillus reuteri can form a small amount of biofilm in the medium without additives, and the film-forming ability is weak. Both brown algae extract and mannan oligosaccharide can significantly improve the film-forming ability of Lactobacil...

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Abstract

The invention discloses a composition based on brown algae extract and lactobacillus reuteri and application of the composition. The composition disclosed by the invention is prepared from the following components in parts by weight: 30 to 97 parts of brown algae extract and 3 to 70 parts of lactobacillus reuteri. The combination of the brown algae extract and the lactobacillus reuteri can promote field planting of the lactobacillus reuteri in intestinal tracts, and the field planting rate is remarkably increased. The combination of the brown algae extract and the lactobacillus reuteri can significantly reduce the fasting blood glucose and glycosylated hemoglobin concentration of type 2 diabetes model mice, improve the insulin secretion amount, significantly improve the abundance of beneficial bacteria such as bifidobacterium and lactobacillus in intestines, reduce the abundance of conditioned pathogenic bacteria such as enterococcus and proteobacteria. Good application prospects are realized.

Description

technical field [0001] The invention relates to a composition based on brown algae extract and Lactobacillus reuteri and its application, belonging to the field of biotechnology. Background technique [0002] Lactobacillus plantarum is an intestinal probiotic that can colonize the intestinal tract and exert a probiotic effect. Studies have found that Lactobacillus reuteri has a good ability to regulate metabolism, oral administration of Lactobacillus reuteri can improve animal lipid metabolism and intestinal permeability, prevent obesity and improve lipopolysaccharide endotoxemia. Preliminary clinical trials also show that Lactobacillus reuteri is safe, and appropriate supplementation can significantly reduce insulin secretion and total cholesterol levels in overweight and obese patients, and reduce insulin resistance. The effect of Lactobacillus reuteri on improving disease symptoms has also been reported in succession. Lactobacillus reuteri is closely related to the phys...

Claims

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Application Information

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IPC IPC(8): A61K36/03A61P1/00A61P3/10A61P31/00A61K35/747
CPCA61K36/03A61K35/747A61P1/00A61P3/10A61P31/00A61K2236/19A61K2300/00
Inventor 李恒吴轶凡俞嘉乐丁一珍刘顺吴艳丽郑可欣史劲松许正宏
Owner JIANGNAN UNIV
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