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Extraction method of dietary fiber from sweet potato dregs as well as application thereof in adjusting intestinal flora

A technology for regulating intestinal flora and dietary fiber, applied in food extraction, application, function of food ingredients, etc., can solve problems such as environmental pollution, economic loss, emission, etc., to increase colonization capacity, increase water content, and increase ratio Effect

Inactive Publication Date: 2019-07-19
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In China, sweet potatoes are mainly used for the processing of starch products, and the by-product sweet potato residue is often used as animal feed or directly discharged with wastewater, causing huge economic losses and environmental pollution

Method used

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  • Extraction method of dietary fiber from sweet potato dregs as well as application thereof in adjusting intestinal flora
  • Extraction method of dietary fiber from sweet potato dregs as well as application thereof in adjusting intestinal flora
  • Extraction method of dietary fiber from sweet potato dregs as well as application thereof in adjusting intestinal flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Experimental procedures: 1) Shandong Hongba sweet potatoes were purchased from farmers’ markets; 2) Wash the sweet potatoes and cut them into sweet potato pieces with a length, width and height of 5cm; 3) Put the sweet potato pieces and water in a high-speed beater at a weight ratio of 1:2 Crush; 4) After the above-mentioned beating liquid is filtered through two layers of gauze, the residue is collected, which is the sweet potato residue; 5) The sweet potato residue is washed 4 times with tap water to fully remove the residual starch; 6) The sweet potato residue is dried in an oven at 60°C Dry to constant weight; 7) Put the dried sweet potato residue into a pulverizer to pulverize, pass through a 100-mesh sieve, and store at 4°C for later use. The yield of sweet potato residue was 3.7%.

Embodiment 2

[0034] Weigh 1.0 g of sweet potato residue, add 40 mL of MES-TRIS buffer solution (0.05 mol / L, pH 8.2, 24° C.), stir and disperse for 1 hour with a magnetic stirrer. Add 50 μL of α-amylase (3000 U / mL) to remove the starch dissolved in the potato residue, and enzymatically hydrolyze in a water bath at 95°C for 35 minutes. Add 100 μL of protease solution (50 mg / mL), and enzymatically hydrolyze in a water bath at 60 °C for 30 min. Adjust the pH to 4.5 with 3 mol / L acetic acid, add 100 μL amyloglucosidase, and enzymatically hydrolyze in a water bath at 60°C for 30 minutes. After the enzymatic hydrolysis is completed, suction filter and collect the filtrate, wash twice with 70°C pure water (about 10 mL), combine the filtrate, add 4 times the volume of 95% ethanol (60°C), stir while adding, and alcohol precipitation at room temperature for 1 h, Centrifuge (3000rpm, 15min), and the precipitate is sweet potato residue dietary fiber after drying. The yield of sweet potato dietary fib...

Embodiment 3

[0036] 3.1 In vitro model to simulate human digestion process

[0037] Accurately weigh 12g of sweet potato dregs dietary fiber and place it in an Erlenmeyer flask, add 200mL of distilled water, stir evenly, add α-amylase, shake the water bath at 37°C for 30min, then adjust the pH to 2.0 with HCl, add 0.54g of pepsin, and Shake in a water bath at 37°C for 2 hours; after the end, adjust the pH to 6.8, add pancreatin and bile, mix well, dialyze, freeze-dry, and store for in vitro simulated intestinal fermentation experiments.

[0038] 3.2 In vitro model to simulate intestinal fermentation process

[0039] Add 90 mL of basal medium to a sterile fermentation bottle, and pre-reduce overnight at 37°C in an anaerobic workstation. Rat feces were aseptically collected, put into sterilized EP tubes, and immediately diluted with pre-reduced phosphate buffer saline (PBS, 0.1mol / L, pH7.0) at a ratio of 1:9. Take 10mL of feces dilution and add it to the pre-reduced basal medium, and the s...

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Abstract

The invention provides an extraction method of dietary fiber from sweet potato dregs. The method can be used to produce high purity dietary fiber in large quantity from the sweet potato dregs, a by-product of sweet potato processing, the process is simple, and productivity is high. The invention also provides an application of the dietary fiber from sweet potato dregs in adjusting intestinal flora, and a simulation experiment of intestinal fermentation in vitro shows that the dietary fiber from sweet potato dregs can improve the colonization ability of intestinal beneficial bacteria and reducethe colonization ability of opportunistic pathogens, and that the effect of prebiotics on the dietary fiber from sweet potato dregs is significant; an animal level experiment shows that, after beingfed the dietary fiber from sweet potato dregs for 28 D, abundance of intestinal flora at phylum, genus and OTU classification level in rats has significant changes, the water content of colon feces rises, the pH of contents drops, the short chain fatty acid content of colon feces rises. Moreover, both villus height and recess depth of small intestine change, the consequence of the experiments showthat the dietary fiber from sweet potato dregs not only improves the health level of intestinal flora, but also enhances the absorption and digestion function of small intestine.

Description

technical field [0001] The invention relates to the extraction and application of dietary fiber, in particular to a method for extracting dietary fiber from sweet potato residue and its application in regulating intestinal flora. Background technique [0002] Sweet potato is rich in nutrients, rich in starch, sugar, protein, vitamins, cellulose and various amino acids, it is a very good nutritional food. Sweet potatoes are widely distributed in China, with the most in the Huaihai Plain, the Yangtze River Basin and the southeastern coastal provinces. my country is the largest sweet potato producer in the world. The perennial sweet potato planting area is 75-80 million mu, and the sweet potato production accounts for about 80% of the world's total production. In China, sweet potatoes are mainly used for the processing of starch products, and the by-product sweet potato residue is often used as animal feed or directly discharged with wastewater, causing huge economic losses and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/21
CPCA23V2002/00A23L33/21A23V2200/32A23V2250/5116A23V2300/14A23V2300/10
Inventor 李学志刘曼周思含田俊邱欣周康杨坤龙李永新
Owner XUZHOU NORMAL UNIVERSITY
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