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Biological agent for preventing strawberry powdery mildew and preparation method thereof

A technology for strawberry powdery mildew and biological agents, applied in botany equipment and methods, biocides, chemicals for biological control, etc., can solve low food safety, excessive pesticide residues in plants and soil, and unfavorable long-term development of soil and other issues, to achieve the effects of increasing yield, benefiting prevention and treatment, and benefiting crop yield

Inactive Publication Date: 2019-11-22
嘉特(烟台)生态农业科技研发有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are still pesticides and fungicides that lead to excessive pesticide residues in plants and soil, low food safety, and are not conducive to the long-term development of the soil. A biological agent for the control of strawberry powdery mildew that regulates plant vegetative growth and reproductive growth and increases crop production is hereby developed. and its preparation method is crucial

Method used

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  • Biological agent for preventing strawberry powdery mildew and preparation method thereof
  • Biological agent for preventing strawberry powdery mildew and preparation method thereof

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Embodiment approach

[0026] The principles and features of the present invention are described below, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0027] A biological preparation for preventing and treating strawberry powdery mildew mainly consists of Harpin protein, Bacillus subtilis fermentation liquid and adsorption carrier components. Through the compounding of Harpin protein and Bacillus subtilis bacterial liquid, combining the advantages of the two, not only can prevent the occurrence of strawberry powdery mildew, have the function of disease resistance, but also can cure strawberry powdery mildew, and have the ability to assist in the treatment of diseases. At the same time, Harpin protein can provide nutrition for crops, promote plant growth, increase crop yield, and realize the effect of one drug with multiple functions. The weight ratio unit of the components in this application is uniformly adop...

Embodiment 1

[0044] A biological agent for preventing and treating strawberry powdery mildew mainly consists of 10kg of Harpin protein, 50kg of fermentation liquid of Bacillus subtilis and 25kg of light calcium carbonate.

[0045] A method for preparing a biological agent for preventing and treating strawberry powdery mildew, comprising the following steps,

[0046] Step 1, selecting the Bacillus subtilis strain, activating, expanding cultivation and fermentation, and obtaining the Bacillus subtilis fermentation liquid after cell lysis;

[0047] Described bacterial classification activation adopts solid plate culture, and the substratum that solid plate culture adopts is PDA solid medium, and PDA solid medium comprises 200g potato, 20g glucose or sucrose as raw material, and potato is boiled in water 30min and filters, and after filtering, take Add 20g of glucose or sucrose and 20g of agar to the supernatant, and add water to make up to 1000mL, sterilize at 123°C and pressure of 0.132MPa f...

Embodiment 2

[0056] A biological agent for preventing and treating strawberry powdery mildew mainly consists of 12kg of Harpin protein, 60kg of fermentation liquid of Bacillus subtilis and 30kg of light calcium carbonate.

[0057] A method for preparing a biological agent for preventing and treating strawberry powdery mildew, comprising the following steps,

[0058] Step 1, selecting the Bacillus subtilis strain, activating, expanding cultivation and fermentation, and obtaining the Bacillus subtilis fermentation liquid after cell lysis;

[0059] Described bacterial classification activation adopts solid plate culture, and the substratum that solid plate culture adopts is PDA solid medium, and PDA solid medium comprises 200g potato, 20g glucose or sucrose as raw material, and potato is boiled in water 30min and filters, and after filtering, take Add 20g of glucose or sucrose and 20g of agar to the supernatant, add water to make up to 1000mL, sterilize at 125°C and pressure of 0.168MPa for 0...

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Abstract

The invention relates to a biological agent for preventing and treating strawberry powdery mildew and a preparation method thereof, and belongs to the field of disease prevention and treatment. The biological agent is mainly composed of Harpin protein, bacillus subtilis fermentation liquid and an adsorption carrier component, and not only can prevent and protect strawberry powdery mildew, but alsocan cure and protect strawberry powdery mildew. The Harpin protein can enable plants to generate disease-resistant, insect-pest-resistant and stress-resistant effects, and active substances generatedin the growth process of the bacillus subtilis have an obvious inhibiting effect on pathogenic bacteria or conditioned pathogenic bacteria of endogenous infection, so that prevention and treatment ofpowdery mildew of strawberries are facilitated. In addition, the biological agent can provide nutrition for crops, automatically adjust vegetative growth and reproductive growth of the crops and improve the yield of the crops. The Harpin protein in the biological agent is high-resistance immune protein, can provide nutrition for crops, can promote plant growth and is beneficial to increase of crop yield.

Description

technical field [0001] The invention relates to a biological agent for preventing and treating strawberry powdery mildew and a preparation method thereof, belonging to the technical field of disease prevention and control. Background technique [0002] Strawberry powdery mildew is a common and important disease of strawberries, especially in low-temperature and high-humidity environments. The incidence of powdery mildew is very high. Generally, it can cause a 20% to 30% reduction in strawberry field production. In severe cases, it can reduce production by 50%, or even fail. Seriously affected the planting benefits of strawberry growers. [0003] At present, the measures to prevent and control strawberry powdery mildew include agricultural control, mainly including reasonable crop rotation, selection of disease-resistant varieties, reasonable fertilization, and rational irrigation. The main control measures are chemical control. The large-scale use of strawberry and soil pes...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N61/00A01N25/08A01N25/14A01P3/00A01P21/00
CPCA01N25/08A01N25/14A01N61/00Y02A40/10
Inventor 宫玉胜董战孙燕霞丛瑜王作全周祖华
Owner 嘉特(烟台)生态农业科技研发有限公司
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