The invention discloses a cultivation method of an Arabian jasmine flower penjing planted with old dwarf trees, and belongs to the technical field of penjing cultivation. To solve the technical problems that for an Arabian jasmine flower penjing planted with old dwarf trees which is cultivated by the prior art, flowers are few, quality is poor, branches are easily withered, survival rate is low, and cultivation cost is high, the following steps of S1, Arabian jasmine flower old dwarf trees selection, S2, matrix selection, S3, cultivation, S4, water and fertilizer management, S5, topdressing and pruning, and S6, finished product marketing are adopted. Nutrient soil of the penjing is screened, red soil, mixture of mulberry twigs and bagasse pith, and powdered carbon are selected as matrix, and Arabian jasmine flowers grow vigorously. The mulberry twigs which are the by-products of mulberry planting and silkworm raising, the bagasse pith which is the by-product of cane sugar manufacture, and wood processing tails are fully utilized, the obtained cultivation matrix is fertile and low in cost, large-scale operation can be achieved, and the cultivation method is easily promoted and applied. According to the cultivation method of the Arabian jasmine flower penjing planted with old dwarf trees, pot culture survival rate can be increased, cultivation survival rate is as high as 99%, the Arabian jasmine flower penjing is easily supported after being bought by customers, and ornamental value of the Arabian jasmine flower penjing is largely increased.