Method for breeding male sterile line and maintainer line of kale
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A male sterile line, kale technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of long time, labor and time, lack of feasibility, etc., achieve good results, and speed up the transformation. process, the effect of shortening the breeding time
Inactive Publication Date: 2013-12-25
ZHENJIANG SUIHAN AGRI
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This method takes a long time, and it takes more than 15 generations to obtain the male sterile line and t
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Embodiment 1
[0028] Selected commodity ornamental kale variety HG-11 (HG-11 is purchased from Japan Takii (Takii) Seedling Co., Ltd. 1 つぐみ, (つぐみ translated into Chinese is "spotted thrush"), HG-11 is the code in the breeding process), round leaves, green outer leaves, pink heart leaves; selection of cabbage cytoplasmic male sterile material C-12 ( C-12, also known as Zhonggan No. 22, produced by Vegetable and Flower Research Institute of Chinese Academy of Agricultural Sciences) as a sterile source, see figure 2 Be explained.
[0029] 1. Ornamental kale variety HG-11 (F 1 つぐみ), observe the offspring BF after selfing the first generation 1 Separation and change of traits. Select horizontal plant type, round leaves, green outer leaves, red heart leaves, good ornamental value, cold resistance, late bolting, strong disease resistance, large pollen amount, and good compatibility as the breeding targets. Select individual plants, BF 1 Orientation is preferred for single plants to carry out ...
Embodiment 2
[0040] Select commercial ornamental kale variety MH (MH is also known as "Osaka White" from Japan, purchased from Zhejiang Hongyue Flower Co., Ltd.) with wavy leaves, dark green outer leaves and white heart leaves, and broccoli cytoplasmic male sterile material CMS034 ( Source: Broccoli "Excellent", produced by Japan SAKATA (Sakata) Company) as a source of sterility. see image 3 Be explained.
[0041] 1. Ornamental kale variety MH, after selfing the first generation, observe the offspring BF 1 Separation and change of traits. Select horizontal plant type, wrinkled leaves, green outer leaves, white heart leaves, good ornamental, cold resistance, strong disease resistance, large pollen amount, and good compatibility as the breeding targets. Directional selection Strains, BF1 orientation preferred single plant for the second generation of selfing.
[0042] 2. In the self-bred offspring group BF 2 After selection and confirmation, the individual plants continue to self-fertil...
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Abstract
The invention discloses a method for breeding a male sterile line and a maintainer line of kale. The method mainly comprises the following steps: selecting a kale variety; determining a breeding goal after the first generation of selfing; directionally selecting a single plant and performing the second generation of selfing; directionally selecting a single plant from selfing progenies and continuing to self; directionally selecting the single plant according to the breeding goal; meanwhile, performing interspecific hybridization with a cytoplasmic male sterile line as a female parent and the selected single plant as a male parent to obtain hybrid F1, so that the CMS (cytoplasmic male sterility) gene of the sterile line is transferred to the kale; purifying by continuing to self the selfed progenies; meanwhile, screening the sterile plant, which is closest to the male parent breeding goal, from cytoplasmic male sterile hybrid plant population to serve as the female parent, screening the single plant, which is closest to the breeding goal, from the selfed progenies as the male parent, and continuing to perform hybridization in pairs; and purifying the selfed progenies and transferring the male sterile line of each generation in pairs at the same time, thus obtaining the maintainer line and the male sterile line after selfing for a plurality of generations. By the method, labor and cost can be reduced remarkably, and breeding time can be shortened.
Description
technical field [0001] The invention belongs to the technical field of flower breeding, in particular to a method for breeding male sterile lines and maintainer lines of kale. technical background [0002] Ornamental kale (Brassica oleracea var.acephala f.tricolor H or t.) is a variety of Brassica oleracea in the Brassicaceae family. It is native to the European Mediterranean to the North Sea coast. The shape is beautiful and changeable, the heart and leaves are colorful like flowers, and the cold resistance is strong. It is the first choice for viewing in late autumn, winter and early spring, and has high ornamental value and economic benefits. [0003] Kale is a typical cross-pollination crop with obvious heterosis. Using heterosis is one of the fast and effective ways to breed new varieties of kale, especially the use of cytoplasmic male sterile lines to prepare hybrids, which can avoid Artificial emasculation saves time and labor, the hybridization rate can reach 100%, ...
Claims
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