Omega-9 quality brassica juncea

a brassica juncea, omega technology, applied in the field of improved brassica species, can solve the problems of reducing the marketable value of oil, shortening the operation time, and not all fatty acids in vegetable oil are equally vulnerable to high temperature and oxidation

Inactive Publication Date: 2013-11-21
CORTEVA AGRISCIENCE LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0016]Alternative aspects of the invention include plants and plant parts. As used herein, “plant parts” includes plant cells, seeds, pollen bearing the nucleic acids of the invention or having the fad2 / fad3 coding sequences of the invention or having regulatory sequences, such as sequences upstream of FAD2 / FAD3 coding regions, that express FAD2 and / or FAD3 enzymes from Brassica napus. Methods are provided for using the plants of the invention, including progeny plants selected by markers of the invention, to obtain plant products. As used herein, “plant products” includes anything derived from a plant of the invention, including plant parts such as seeds, meals, fats or oils, including plant products having altered oleic acid and linolenic acid concentrations. Methods are provided for modifying plants so that they have transferred fad2 / fad3 coding sequences from Brassica napus capable of expressing an active FAD2 enzyme and / or FAD3 enzyme. Such methods may for example involve transferring one or more of the fad2-a and / or fad3-a coding sequences from Brassica napus in a plant through interspecific hybridization, so that the plant has substituted fad2 and / or fad3 coding sequences. Such methods allow identification and precise introgression of derived mutations into Brassica juncea.

Problems solved by technology

Further, not all fatty acids in vegetable oils are equally vulnerable to high temperature and oxidation.
Poor oxidative stability brings about, for example, shortened operation times in the case where the oil is used as a fry oil because oxidation produces off-flavors and odors that can greatly reduce the marketable value of the oil.
Other limitations of the related art will become apparent to those of skill in the art upon a reading of the specification and a study of the drawings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Backcrossing

[0137]Referring to FIG. 1, one or more backcrosses (BC3 and BC4) between the high oleic—low linolenic selections and B. juncea parents (Zem1, Zem2 and ZE Skorospelka) in order to fully recover B. juncea genetic background. Only zero erucic B. juncea lines were used in the backcrossing program since this would allow for full expression of the fad2 and fad3 mutant alleles in a non competitive situation with the FAE gene(s).

[0138]After each advanced back-cross (for example BC3, BC4) and subsequent self pollination (for example BC3F2, BC4F2), progeny seed is subjected first to tissue screening for presence of fad2a and fad3a genes (using markers as described in more detail herein), then grown on to flowering to be used in the subsequent backcrosses. Harvested seed from selected lines is subjected to oil profile analysis using half seed, non-destructive single whole seed NIR analysis, or single seed NIR (FTNIR). Subsequently, samples containing increased oleic levels, and red...

example 2

Development of B. Juncea-Specific Markers

[0145]DNA Markers are developed that can detect presence of the BB genomic DNA relevant to FAD2b and other available sequences from B. nigra and B. juncea (representing the BB genome). Double haploid mapping populations are developed for marker development. In addition, DNA (SSR and SNP) markers are developed from the known B-genome sequences. These markers are able to confirm the extent of recovery of B. juncea background in the converted lines.

[0146]A total of 1931 B. napus SSR markers, predominantly containing, di- and tri-nucleotide repeat motifs, are available for parental screening. These markers are currently being screened on a panel of Brassica lines that belong to B. juncea (Zem1, Zem2 and ZE Skorospelka lines), Omega-9 B. napus, B. rapa, B. nigra, and B. oleracea. This screening provides two types of information. First, since these SSR markers were developed from B. napus genome, the screening provides information on their utility ...

example 3

Recovery and Determination of B Genome

[0151]Self-pollinated and doubled haploid plants exhibiting seed oil profile of high oleic and low linolenic acids, as previously described, are screened using the markers selected for the BB genome. The BB genome is confirmed present in the converted lines. These mutant B. juncea lines also show a decrease (or complete absence of) in the number of positive C genome markers selected for B. napus. These profiles are further enhanced by additional backcrosses and selfing techniques known in the art which improve the agronomics of the line, e.g., reduce yield drag, reduce pod shatter, alter maturity for various growing zones, increase stress tolerance, increase disease resistance, and the like.

[0152]Three different methods are used for the determination of B genome in the self-pollinated and DH progeny from B. napus and B. juncea interspecific crosses exhibiting desired seed oil profile.

A) Molecular Markers

[0153]Molecular markers capable of detecti...

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Abstract

The invention relates to improved Brassica species, including Brassica juncea, improved oil and meal from Brassica juncea, methods for generation of such improved Brassica species, and methods for selection of Brassica lines. Further embodiments relate to seeds of Brassica juncea comprising an endogenous oil having increased oleic acid content and decreased linolenic acid content relative to presently existing commercial cultivars of Brassica juncea, seeds of Brassica juncea having traits for increased oleic acid content and decreased linolenic acid content in seed oil stably incorporated therein, and one or more generations of progeny plants produced from said seeds.

Description

CROSS-REFERENCE TO RELATED APPLICATION[0001]This application is a divisional of U.S. patent application Ser. No. 12 / 590,197, filed Nov. 4, 2009, pending, which application is a utility conversion of U.S. Provisional Patent Application Ser. No. 61 / 198,422, filed Nov. 4, 2008, for “Omega-9 Quality Brassica Juncea,” the entire disclosure of each of which is hereby incorporated herein by this reference.FIELD OF THE INVENTION[0002]The invention is in the field of improved Brassica species, including Brassica juncea, improved oil and meal from Brassica juncea, methods for generation of such improved Brassica species, and methods for selection of Brassica lines. Further embodiments relate to seeds of Brassica juncea comprising an endogenous oil having increased oleic acid content and decreased linolenic acid content relative to presently existing commercial cultivars of Brassica juncea, and seeds of Brassica juncea having traits for increased oleic acid content and decreased linolenic acid...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C11C1/00A01H5/10A23L25/00
CPCC11C1/002A01H5/10A01H6/201A01H1/045
Inventor RIPLEY, VAN LEONARDTHOMPSON, STEVEN ARNOLDEHLERT, ZOE CHRISTINA
Owner CORTEVA AGRISCIENCE LLC
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