Method for introducing radish chromosomes into cabbage

A chromosome and chromosome number technology, applied in the field of cabbage distant hybridization and utilization, can solve problems such as the inability of chromosomes to be transferred, and achieve the effects of overcoming the obstacles of distant hybridization, avoiding genetic burden, and improving the efficiency of transfer and selection.

Inactive Publication Date: 2011-08-03
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with sexual hybridization, although protoplast fusion technology can overcome the incompatibility of distant hybridization to a certain extent and obtain hybrids, it is still difficult to achieve in terms of the technical maturity of protoplast culture, the genetic stability and fertility

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for introducing radish chromosomes into cabbage
  • Method for introducing radish chromosomes into cabbage
  • Method for introducing radish chromosomes into cabbage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] Such as figure 1 As shown, the specific steps are as follows:

[0025] 1. Cross-pollination and ovary cultivation: take Brassica napus-radish d chromosome addition line as female parent, cabbage (commercially available to buy, variety or homozygous inbred line can be applied to the present invention, without specific limitation) Cross-pollinate the male parent. 2 to 3 days before flowering, the flowering branches of the parents were bagged and isolated separately. The female parent is emasculated at the bud stage, and the pollen of the male parent is taken and applied to the stigma of the female parent. The ovaries 7-15 days after pollination were taken for tissue culture. Medium: 1 / 2MS+inositol (10g / L)+nicotinic acid (100mg / L)+VB6(100mg / L)+VB1(1g / L)+IAA(1.5mg / L)+sucrose (50g / L ), pH=5.8. After cultivating for about a month, the seeds in the ovary are basically mature, and one generation is obtained.

[0026] 2. Identification of the first generation of transfer: ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for introducing radish chromosomes d into cabbage. The method comprises the following steps of: performing sexual hybridization on a cabbage type rape-radish chromosome d addition line and cabbage; introducing the radish chromosomes d into the cabbage; selecting a cabbage plant containing the radish chromosomes d by adopting a molecular marker detection and cell microscopy method; and creating a new cabbage germplasm carrying the radish chromosomes d. In the method, cabbage type rape is taken as a carrier of the radish chromosome d, and is hybridized with cabbage to transfer the radish chromosome d to the cabbage, so that direct hybridization between the cabbage and radish is avoided. Hybridization in brassica alboglabra succeeds easier than intergeneric hybridization between brassica alboglabra and radish. Moreover, only one pair of radish chromosomes is integrated with a cabbage genome, so that more genetic drag caused by the hybridization of an entire radish genome can be avoided, and the transfer and selection efficiencies of target traits can be improved. By adopting the method, a novel thought is provided for distant hybridization utilization.

Description

technical field [0001] The invention relates to a cultivation method of cabbage, in particular to a method for introducing d-chromosome of radish into cabbage, and belongs to the field of distant hybridization and utilization of cabbage. Background technique [0002] Distant hybridization refers to the hybridization between distantly related types, including hybridization between species, genera and families. It can create specific types or new germplasm that existing plants do not have, so distant hybridization has become an important means to solve the shortage of plant genetic resources, and has been widely used in plant genetics and breeding, such as in wheat (Bris B. et al., 1983; Kozub NA et al., 2004), cotton (Vmh B I et al., 1999), rape (Liu Zhongsong et al., 2001; Chen Shuzhong et al., 2000), cucumber (Chen J F et al., 1997) and other crops through interspecific or intergenus hybridization Variety improvement has been carried out to obtain rich genetic variation ty...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H1/02C12Q1/68
Inventor 丁云花李利军李倩简元才康俊根
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap