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Method for promoting fertility of distant hybridization generation of paddy and applied protein

A distant hybridization, protein technology, applied in chemical instruments and methods, botanical equipment and methods, biochemical equipment and methods, etc.

Active Publication Date: 2019-04-26
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hybrid weak genes Hwi1 and Hwi2 were identified through the introgression lines constructed from common wild rice and Asian cultivated rice, but there is no report on the isolation of female sterility genes from the hybrid offspring of common wild rice and Asian cultivated rice

Method used

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  • Method for promoting fertility of distant hybridization generation of paddy and applied protein
  • Method for promoting fertility of distant hybridization generation of paddy and applied protein
  • Method for promoting fertility of distant hybridization generation of paddy and applied protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1. Reducing the expression of the gene encoding ESA1 can improve the female gamete fertility of hybrid offspring of wild rice and cultivated rice

[0067] In this example, a protein derived from O. sativa Yuanjiang was discovered, and its name was ESA1. Reducing the expression of the protein-coding gene in offspring of hybrids between O. The amino acid sequence of ESA1 is sequence 1 in the sequence listing. In Yuanjiang wild rice, the genome sequence of the ESA1 gene is sequence 2 in the sequence listing, and the CDS sequence is sequence 3 in the sequence listing, the 83-1903 coding sequence 1 of sequence 3. ESA1 shown.

[0068] 1. Preparation of semi-sterile infiltration line YIL42

[0069] Using Yuanjiang wild rice as the donor parent and the indica rice Teqing as the recurrent parent, a set of introgression populations containing 127 lines was constructed through crossing and backcrossing for 4 generations, and a plant with a low seed setting rate was select...

Embodiment 2

[0112] Embodiment 2, ESA1 gene expression detection

[0113] The plant to be tested is: the near-isogenic line NIL-qSSR1, which is obtained by using YIL42 as the donor parent and the indica rice Teqing as the recurrent parent through hybridization and backcrossing to exclude other chromosome introgression segments. The near isogenic line contains the ESA1 gene.

[0114] Extract the total RNA of the plant to be tested, and reverse transcribe it into cDNA, use primers (5'-TGGAACAGAAGAAAGGCTTG-3' (forward primer), 5'-AGCCACATCCTCAAGATGAA-3' (reverse primer)) to detect the expression level of ESA1 gene, use The Actin gene was used as an internal reference, and the internal reference primers were: 5'-TACAGTGTCTGGATTGGAGGAT-3' (forward primer) and 5'-TCTTGGCTTAGCATTCTTGGGT-3' (reverse primer).

[0115] The result is as figure 2 shown. The results showed that the relative expression level of NIL-qSSR1 in the panicle was higher in the P8-P13 period (that is, the panicle length was...

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Abstract

The invention discloses a method for promoting fertility of distant hybridization generation of paddy and an applied protein. The method for promoting fertility of distant hybridization generation ofpaddy disclosed by the invention comprises the step of reducing content of ESA1 in receptor paddy or restraining activity of ESA1 in receptor paddy, thereby acquiring a target paddy with fertility higher than that of the receptor paddy and realizing enhancement of paddy fertility. The receptor paddy is the distant hybridization generation of paddy. ESA1 is the protein shown as a sequence 1 in a sequence table. An experiment proves that the maturing rate of the distant hybridization generation of paddy can be increased, the fertility of distant hybridization generation of paddy can be promotedand the obstacle of distant hybridization of wild paddy and cultivated paddy can be overcome after the expression of ESA1 encoding gene in the invention is restrained. The method has an important significance in effectively utilizing excellent genes of wild paddy to improve the cultivated paddy.

Description

technical field [0001] The invention relates to a method for improving the fertility of rice distant hybrid offspring and the protein used in the field of biotechnology. Background technique [0002] Rice is one of the most important food crops in the world, accounting for 50% of the world's population as a staple food. Increasing rice yield per unit area is one of the powerful measures to ensure world food security. The narrow genetic diversity among rice varieties has become a bottleneck restricting the further improvement of rice yield potential. Wild rice is an important beneficial gene pool, which carries genes for resistance to diseases and insect pests, stress resistance, high yield and high quality. Using the favorable genes of wild rice through distant hybridization is an effective way to improve the level of rice breeding. However, due to the phenomenon of sterility or semi-sterility in wild rice and cultivated rice hybrids and hybrid offspring, the effective use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/46A01H1/02
CPCA01H1/02C07K14/415C12N15/8218
Inventor 付永彩侯晶晶刘雅馨刘凤霞谭禄宾朱作峰才宏伟孙传清
Owner CHINA AGRI UNIV
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