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Method for cultivating high-fecundity allotetraploid corn

An allotetraploid and autotetraploid technology, applied in the field of cultivating high seed-bearing allotetraploid maize, can solve the problem of inability to guarantee the number of offspring and effectively preserve beneficial germplasm, uncoordinated growth of offspring, and inability to self-determine. Cross-fertilization and other problems, to achieve the effect of large seeds, stable offspring traits, and full seeds

Active Publication Date: 2020-04-10
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the progeny produced by hybridization of the near allotetraploid maize has problems such as uncoordinated growth, low seed setting rate, and failure to self-seed and fruit due to failure of flowering, unable to guarantee the number of offspring and effectively preserve beneficial germplasm.
Therefore, the low seed setting rate of offspring has become the main obstacle to transfer the excellent traits of maize relatives to maize materials or to apply them in maize breeding.

Method used

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  • Method for cultivating high-fecundity allotetraploid corn
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  • Method for cultivating high-fecundity allotetraploid corn

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Embodiment 1 Breeding of high-fruiting allotetraploid corn of the present invention

[0034] Follow the steps below:

[0035] (1) In June 2016, BF7 (preservation number: CGMCC No.18176, 2n=38~41) was used as the female parent and P1-vv (quoted from CIMMYT) as the male parent in Wenjiang Farm of Sichuan Agricultural University Crossbreed, harvest seeds when mature, get F 1 generation.

[0036] (2) In September 2016, F was taken out of the -20°C refrigerator 1 The generation seeds were planted in Xishuangbanna Breeding Base of Sichuan Agricultural University. 1 generation; take F from mid-September to mid-October 2016 1 Genomic in situ hybridization method (see Tang Q et al. Crop Sci, 2005, 45: 717-721) was used to identify the number of chromosomes and chromosome composition, and select F 1 The total number of chromosomes in the generation materials is 38-42, of which the number of chromosomes in maize (hereinafter referred to as: Mz) is 26-32, the number of chromos...

Embodiment 2

[0039] Embodiment 2 Application of the high-fruiting allotetraploid corn of the present invention

[0040] (1) In March 2018, 30 high-fruiting allotetraploid corn materials obtained in Example 1 were planted in Wenjiang Farm, Sichuan Agricultural University, and 30 corn inbred line Mo17 were planted at the same time. In May 2018, the The high-fruiting allotetraploid corn material obtained in Example 1 was used as the female parent, and Mo17 was used as the male parent for hybridization, and all hybrids could bear fruit; harvested in June 2018, the hybrid F 1 Generation, F 1 The grain shape of the generation is large and full.

[0041] (2) In September 2018, F obtained from step (1) was planted in Xishuangbanna Breeding Base of Sichuan Agricultural University 1 Substitute seed; November 2018 with F 1 As female parent, backcrossed with Mo17 as male parent, harvested in February 2019, obtained BC 1 generation seeds.

[0042] (3) In March 2019, the BC obtained in the planting...

Embodiment 3

[0044] Embodiment 3 Yield comparative test of different allotetraploid corns cultivated by the method of the present invention

[0045] (1) According to the method described in Example 1, BF7 is the female parent, and the autotetraploid corn P1-mm or Twf9 (both cited from CIMMYT) is used as the male parent to cross, and the high seed setting rate of the two families is bred Allotetraploid maize.

[0046] (2) In April 2018, 56 plants of high-fruiting allotetraploid maize were cultivated in two families in the Wenjiang Farm of Sichuan Agricultural University in two rows and three times in the planting step (1).

[0047] (3) In June 2018, the statistics on fertility showed that the average pollen fertility of the two families was 77.6% and 75.4% respectively, and the high-fruiting allotetraploid maize of the two families described in step (2) was automatically pay.

[0048] (4) In August 2018, the harvested seeds were large and full. The test results showed that the average see...

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Abstract

The invention discloses a method for cultivating high-fecundity allotetraploid corn, and belongs to the technical field of corn distant hybridization. According to the method disclosed by the invention, mainly allopolyploid corn BF7 (preservation number is CGMCC No.18176) is used as a female parent, autotetraploid corn is used as a male parent, and hybridization is performed; and then materials which are high in total quantity of chromosomes, high in contained corn, high in tetraploid perennial type corn, high in the quantity of translocation chromosomes and high in fruiting rate in offspringsare subjected to roguing and selfing, and finally, the high-fecundity allotetraploid corn can be cultivated. The allotetraploid corn cultivated by the method disclosed by the invention is high in fecundity, the shortcoming that former closely allotetraploid corn is low in fecundity can be overcome, chromosome exchange and recombination between the corn and the tetraploid perennial type corn can be sufficient and diversified, introduction of good genes into the corn becomes simple and efficient, and a good base is provided for extending corn heredity germplasm base and further cultivating breakthough varieties.

Description

technical field [0001] The invention belongs to the field of corn distant hybrid breeding methods, in particular to a method for cultivating high-fruiting allotetraploid corn. Background technique [0002] Corn is a crop for both food and feed, and it is also an important industrial raw material. With the increase of population, the improvement of people's living standards, and the development of animal husbandry, the demand for corn also increases sharply. Improving yield per unit area is the main way to increase maize yield, but due to the narrow genetic basis of existing maize germplasm, the breeding of breakthrough maize varieties is limited. Therefore, broadening and creating new maize germplasm resources is the key to cultivating high-yielding varieties. [0003] Tetraploid perennial maize (or Zea perennis L.) and Tripsacumdactyloides L. and other wild relatives of maize have formed various excellent characteristics in the long-term evolution process. Teosinte has t...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 唐祈林温小冬赵艳丽穆罕默德·扎法尔·伊克巴尔李影正严旭吴子洲李杨李晓锋何如钰张萍周阳何建美阿米尔·阿里·阿布罗
Owner SICHUAN AGRI UNIV
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