Method for detecting haloxylon ammondendron seed vitality

A technology of vitality and seeds, applied in the direction of germination equipment, etc., can solve the problem of reduced germination activity and achieve the effect of convenient operation

Inactive Publication Date: 2015-12-23
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the determination of the viability of Haloxylon seeds mostly adopts the method of germination. With the prolongation of storage time of Haloxylon seeds, the germination activity decreases, and it usually takes 3-7 days to detect the viability of Haloxylon seeds.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The optimization experiment of embodiment 1, Haloxylon seed viability assay

[0018] Haloxylon seeds were collected from the wild Haloxylon forest in Alxa League Left Banner, Inner Mongolia.

[0019] The Haloxylon seeds tested were divided into two types, Haloxylon seeds with the seed coat removed and Haloxylon seeds without the seed coat removed.

[0020] 1. Comparison of the dyeing effect of Haloxylon seeds with and without the testa coat removed

[0021] 1. Take Haloxylon seeds, place them in 1% TTC solution at 30°C, and stand in the dark for 12 hours for dyeing.

[0022] 2. After completing step 1, take the seeds, wash them with sterile water, observe and calculate the viability of the seeds (the seeds whose seed coats have been removed are directly observed and counted, and the seeds whose seed coats have not been removed are first removed and then observed and counted).

[0023] Carry out 3 times of repeated tests, and in each repeated test, 200 seeds of the Hal...

Embodiment 2

[0040] Embodiment 2, adopt each parameter optimized in embodiment 1 to carry out the detection of Haloxylon seed viability

[0041] Haloxylon seeds were collected from the wild Haloxylon forest in Alxa League Left Banner, Inner Mongolia.

[0042] The Haloxylon seeds tested were divided into two types, Haloxylon seeds with the seed coat removed and Haloxylon seeds without the seed coat removed.

[0043] 1. Remove the Haloxylon seeds of the testa, place them in 1% TTC solution, and leave to stain for 4 hours at 30° C. in the dark, then get the seeds, wash them with sterile water, observe and calculate the viability of the seeds ( direct observational statistics).

[0044] 2. Take the Haloxylon seeds without removing the seed coat, put them in 1% TTC solution, stand and dye at 30°C in the dark for 24 hours, then take the seeds, wash them with sterile water, remove the seed coats, observe and calculate The viability of the seeds (remove the seed coat first and then observe the s...

Embodiment 3

[0047]Embodiment 3, the detection of the viability of Haloxylon seed collected in different years

[0048] Haloxylon seeds were collected in Alxa League Left Banner in October 2013 and October 2014, respectively.

[0049] One, adopt the method provided by the invention to detect Haloxylon seed viability

[0050] 1. Remove the Haloxylon seeds of the testa, place them in 1% TTC solution, and leave to stain for 4 hours at 30° C. in the dark, then get the seeds, wash them with sterile water, observe and calculate the viability of the seeds ( direct observational statistics). The vitality of the Haloxylon seeds collected in October 2013 was 90%. The vitality of Haloxylon seeds collected in October 2014 was 87%.

[0051] 2. Take the Haloxylon seeds without removing the seed coat, put them in 1% TTC solution, stand and dye at 30°C in the dark for 24 hours, then take the seeds, wash them with sterile water, remove the seed coats, observe and calculate The viability of the seeds (r...

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Abstract

The invention discloses a method for detecting haloxylon ammondendron seed vitality. The provided method for detecting haloxylon ammondendron seed vitality comprises the following steps: dyeing haloxylon ammondendron seeds by using a 2,3,5-Triphenyltetrazolium chloride solution, and obtaining the haloxylon ammondendron seed vitality through the following equation: the haloxylon ammondendron seed vitality is equal to the quantity of the detected seeds of which embryo is dyed red is divided by the quantity of the detected seeds and then is multiplied by 100%. The provided method for detecting haloxylon ammondendron seed vitality overcomes the technical problem that a conventional method for determining the haloxylon ammondendron seed vitality by detecting the germination rate is relatively long in time, possesses characteristics of being convenient and rapid in operation, time-saving and labor-saving, and possesses important economic and application popularization value.

Description

technical field [0001] The invention relates to a method for detecting the vitality of Haloxylon seeds. Background technique [0002] Haloxylon is one of the most widely distributed desert vegetation types in the Asian desert area. It is also a super-xerophyte, extremely drought-resistant and salt-tolerant, and plays an important role in windbreak and sand fixation and ecological restoration. [0003] Haloxylon seed is a short-lived seed with a lifespan of only 10 months when stored at room temperature. In the process of Haloxylon seed storage, germplasm resource preservation and seed quality evaluation, the determination of Haloxylon seed viability is a key technology. At present, the determination of the viability of Haloxylon seeds mostly adopts the method of germination. With the prolongation of the storage time of Haloxylon seeds, the germination activity decreases, and it usually takes 3-7 days to detect the viability of Haloxylon seeds. Contents of the invention ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02
Inventor 张红宋松泉多伟白生玉唐庆立
Owner INST OF BOTANY CHINESE ACAD OF SCI
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