Method for rapidly detecting viability of aeluropus littoralis seeds

A viability and seed technology, which is applied in the field of rapid detection of the viability of Roegrass seeds, can solve problems such as long detection time, and achieve the effects of shortening soaking time, reducing mildew, and reducing mechanical damage.

Active Publication Date: 2015-09-16
SHANDONG AGRI SUSTAINABLE DEV INST
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Problems solved by technology

[0004] The present invention provides a method for quickly detecting the viability of roe chinensis seeds, solves the problem of long detection time in the prior art, can quickly and effectively detect the viability of roe chinensis seeds, and has important practice for grassland utilization and ecological protection significance

Method used

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  • Method for rapidly detecting viability of aeluropus littoralis seeds
  • Method for rapidly detecting viability of aeluropus littoralis seeds

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preparation example Construction

[0025] Solution preparation:

[0026] 1. Preparation method of TTC solution: Prepare two solutions: solution Ⅰ: dissolve 9.078g potassium dihydrogen phosphate (KH2PO4) in 1000ml water; solution Ⅱ: dissolve 9.472g disodium hydrogen phosphate (Na2HPO4) in 1000ml water, or Dissolve 11.876g of disodium hydrogen phosphate (Na2HPO4·2H2O) in water. Take 2 parts of solution I and 3 parts of solution II and mix to obtain a buffer solution. The pH value of the buffer solution is kept in the range of 6.5-7.5. Dissolve tetrazolium powder in the buffer solution in proportion to obtain a definite concentration. Put it into a brown reagent bottle and store it at 4°C for use.

[0027] 2. Preparation method of lactic acid phenol transparent grade: Add 10g phenol into 10mL distilled water, heat to dissolve, then add 10g lactic acid (specific gravity 1.21) and 20g glycerin.

[0028] Calculation method of seed viability of roe chinensis: seed viability of roe chinensis = number of seeds whose...

Embodiment 1

[0031] A method for rapidly detecting the viability of roe chinensis seeds, comprising the following steps:

[0032] (1) First soak 50 roewort seeds (collected in northern Shouguang County, Shandong Province in 2014) in 1.2 mg / L ozone water for 2 minutes, take out and add 8% alkyl glycoside and 12% fatty acid methyl ester ethoxylate Soak the seeds in an aqueous solution, wait for the seeds to swell evenly, and then cross-cut along the 3 / 4 of the seed embryo;

[0033] (2) the TTC solution and the zinc gluconate solution of 0.3% (the zinc gluconate solution used in the present invention is the aqueous solution of zinc gluconate, the following implementation The same as above) according to the mass ratio of 5:1, the mixture was dyed in a dark environment at 30°C, then rinsed twice with distilled water, and then soaked with lactic acid phenol transparent agent at 38°C for 30 minutes. Observe the dyeing condition of the seeds of Roe chinensis, the dyeing is uniform, consistent, br...

Embodiment 2

[0037] A method for rapidly detecting the viability of roe chinensis seeds, comprising the following steps:

[0038] (1) First, soak 50 roewort seeds (collected in Mengcun Hui Autonomous County in the southeast of Hebei Province in 2014) in 1.2mg / L ozone water for 3 minutes, take out and add 5% alkyl glycoside and 15% fatty acid methyl ester B Soak the seeds in an aqueous solution of oxides, wait for the seeds to swell evenly, and then cross-cut along the 3 / 4 of the seed embryo;

[0039] (2) Stain the end of the seed embryo of the roe chinensis seeds with a mixture of TTC solution with a mass percentage of 0.5% and a zinc gluconate solution with a mass percentage of 0.23% according to a mass ratio of 5:1 and place it in a dark environment at 35°C. Then rinse with distilled water for 3 times, and then soak the dyed seeds with lactic acid phenol transparent agent at 38°C for 30 minutes to observe the dyeing of the seeds of roe chinensis. The dyeing is uniform and consistent, and...

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Abstract

The invention belongs to the technical field of seed detection, and in particular relates to a method for rapidly detecting viability of aeluropus littoralis seeds. The method is realized through the following steps of pre-soaking the aeluropus littoralis seeds in ozone water and aqueous solution containing alkyl glycoside and fatty acid methyl ester ethoxylate, then, dyeing one ends of the aeluropus littoralis seeds having seed embryos through mixed solution of TTC (Triphenyl Tetrazolium Chloride) solution and zinc gluconate solution, soaking the dyed seeds by using a lactic acid phenol transparent agent, observing the dyeing condition of the aeluropus littoralis seeds, and calculating the percentage of viability seeds. The method provided by the invention is rapid and effective; the soaking time can be shortened; the mechanical injury to seeds can be reduced; and the method provided by the invention has important practical significance on pasture utilization and ecological protection.

Description

technical field [0001] The invention belongs to the technical field of seed detection, and in particular relates to a method for rapidly detecting the vitality of roe chinensis seeds. Background technique [0002] Swertia chinensis belongs to Gramineae, which is mainly vegetative growth and vegetative reproduction. Stolon is its important vegetative reproductive organ. Compared with it, sexual reproduction makes little contribution to the population of Swertia chinensis, and the ear formation rate is only 15.7%. The seeds are very small, and the thousand-grain weight is only 0.12±0.0015g. It is widely distributed in the inland and coastal saline soil areas of the northern provinces of China. It is a salt-secreting plant among halophytes and is an excellent forage for grazing in sand-fixing and saline-alkali areas. Due to the physiological characteristics of the saline-alkali tolerance and the physiological phenomenon of vegetative reproduction, currently, there are many stu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02
Inventor 贾春林盛亦兵王国良张进红刘洋吴波杨秋玲
Owner SHANDONG AGRI SUSTAINABLE DEV INST
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