Screening and application of yeast CGMCC (china general microbiological culture collection center) 4748 with high ethanol yield and low fusel oil yield in production of Chinese Maotai-flavor liquor

A technology of fusel oil and ethanol, which is applied in the field of bioengineering, can solve problems such as production risks, complex processes, and various influencing factors, and achieve the effects of optimizing production processes, improving wine quality, and reducing production costs
CN102766583AInactive Publication Date: 2012-11-07JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2012-11-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to screening and application of yeast CGMCC (china general microbiological culture collection center) 4748 with high ethanol yield and low fusel oil yield in production of Chinese Maotai-flavor liquor, and belongs to the technical field of biological engineering. The yeast strain is Saccharomyces exiguous CGMCC4748 and is obtained by separating in various yeasts for making hard liquor, fermented grains, fermenting materials and fermenting environments from many Chinese manufacturers of famous liquors. A screening method of the yeast strain includes thinning the yeasts, the fermented grains, the fermenting materials or fermenting environmental materials, coating to a TTC (triphenyltetrazolium chloride) primary-screening flat plate, selecting remarkably red colonies, and transferring the colonies to shaking culture for secondary screening, measuring content of ethanol and fusel oil in fermentation broth by distilling and proportioning process and HS-SPME-GC-MS (head space solid-phase micro-extraction and gas chromatography-mass spectrometry), and screening to obtain the strain with high ethanol concentration and low fusel oil concentration. The strain is applicable to food industry and liquor-making industry.
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Description

technical field

[0001] The invention relates to a method for screening yeasts with high ethanol and low fusel oil yields, and the application of the method to screen yeasts with high ethanol and low fusel oil yields in the production of Chinese Maotai-flavor liquor and its application in food technology and brewing wine industry. It belongs to the technical field of bioengineering. Background technique

[0002] Ethanol is one of the most important and main components in brewing wine. The ethanol content in Chinese liquor is as high as 40%-55% (v / v); in rice wine, it is 14%-20%; in beer, it is 2%-5%. Yeast is the main microorganism that produces ethanol, and it is also an indispensable microorganism in the brewing industry. Therefore, finding high-yielding yeast can optimize the brewing process and reduce production costs, which is of great significance in the brewing industry.

[0003] Fusel oil is a secondary metabolite produced by yeast during brewing fermentation, inclu...

Claims

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