Method for detecting seed viability of orchid plants

A technology for plant seeds and viability, applied to germination equipment and other directions, can solve the problems of long germination cycle, difficult germination, inability to quickly and effectively measure seed viability, etc., and achieve the effect of convenient operation and difficult observation.

Inactive Publication Date: 2009-11-04
INST OF BOTANY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the determination of orchid seed viability mostly adopts the method of germination; because the seed of orchid is composed of embryo and seed coat, without endosperm, this method requires exogenous nutrients under both symbiotic and non-symbiotic germination conditions, and germination is difficult And the germination cycle is long (the non-symbiotic germination time of most orchid seeds needs more than 90 days), so the viability of seeds cannot be measured quickly and effectively

Method used

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  • Method for detecting seed viability of orchid plants
  • Method for detecting seed viability of orchid plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1、 5

[0023] Example 1, the optimization experiment of pretreatment of orchid orchid seeds and dyeing conditions

[0024] Experiment 1. Comparison of the dyeing effects of calcium hypochlorite treatment on the seeds of five lip orchids at different times

[0025] This experiment uses the Ca(ClO) of 5% mass percentage content 2 The solutions were pretreated for 15, 30, 60, 90, and 120 minutes respectively; 1% by mass of 2,3,5-triphenyltetrazolium chloride phosphate buffer solution at 30°C in the dark Dyeing for 24h. The specific method is as follows:

[0026] 1) Pretreatment: Take the fully mature seed pods of Penicillium chinensis, cut open the pericarp and take out the seeds with tweezers, place the seeds in five 2.0mL centrifuge tubes, each about 0.02g, and then add 5 %Ca(ClO) in mass percent 2 Solution (containing 0.8% Tween-20) 1.5mL, cover the lid of the centrifuge tube, and treat for 15, 30, 60, 90, 120min respectively, shake and mix 3-5 times during the period; Push it i...

Embodiment 2

[0054] Embodiment 2, the viability detection experiment of Dendrobium officinalis seed

[0055] This experiment adopts the Ca(ClO) of 5% mass percentage content 2 Seeds were pretreated with the solution for 30 minutes, and then stained with 1% 2,3,5-triphenyltetrazolium chloride phosphate buffer solution for 24 hours at 30°C and in the dark. The specific method is as follows:

[0056] 1) Pretreatment: Take the fully mature seed pods of Dendrobium bougainvillea, cut open the pericarp and take out the seeds with tweezers, place the seeds in a 2.0mL centrifuge tube, each about 0.02g, and then add 5% mass per cent to the centrifuge tube Content of Ca(ClO) 2 Solution (containing 0.8% Tween-20) 1.5mL, cover the lid of the centrifuge tube, treat for 30min, shake and mix 3-5 times during the period; push a small piece of sterile cotton ball into the centrifuge tube with tweezers Slowly push into the lower part of the centrifuge tube, tilt the bottom of the centrifuge tube upwards, ...

Embodiment 3

[0060] Embodiment 3, the viability detection experiment of Dendrobium officinalis seed

[0061] This experiment adopts the Ca(ClO) of 2.5% mass percentage content 2 Seeds were pretreated with the solution for 60 minutes, and then stained with 2% 2,3,5-triphenyltetrazolium chloride phosphate buffer solution for 12 hours at 25°C and in the dark. The specific method is as follows:

[0062] 1) Pretreatment: Take the fully mature seed pods of Dendrobium bougainvillea, cut open the pericarp and take out the seeds with tweezers, put the seeds in a 2.0mL centrifuge tube, about 0.02g per tube, and then add 2.5% mass per cent to the centrifuge tube Content of Ca(ClO) 2 Solution (containing 0.8% Tween-20) 1.5mL, cover the lid of the centrifuge tube, treat for 60min, shake and mix 3-5 times during the period; use tweezers to push a small piece of sterile cotton ball into the centrifuge tube, and the cotton ball Slowly push into the lower part of the centrifuge tube, tilt the bottom of ...

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Abstract

The invention aims at providing a method for detecting seed viability of orchid plants. The method for detecting seed viability of orchid plants provided in the invention comprises the steps of cleaning the orchid plant seeds processed by calcium hypochlorite solution, dyeing the seeds with 2,3,5-triphenyltetrazolium chloride phosphate buffer solution, and then counting the dyeing percentage of the embryos of the seeds, and finally getting the seed viability of the orchid plants. The detecting method solves the technical difficulty that the seeds are not easily operated and observed and difficult to be counted during dyeing measurement as the seeds are small, and has the advantages of convenient and quick operation, time saving and labour saving as well as having important economic value and application and development value.

Description

technical field [0001] The invention relates to a method for detecting the viability of orchid seeds. Background technique [0002] Orchidaceae contains about 800 genera and 25,000 species, widely distributed in tropical, subtropical and temperate regions, mainly in South America and tropical Asia. There are more than 1,000 species of 166 genera in my country, which are mainly distributed in various provinces in the south of the Yangtze River, mostly in Guangdong, Hainan, Yunnan and Taiwan. Orchidaceae are a special group of plants and one of the most evolved families of angiosperms. Among them, bletilla striata and Gastrodia elata can be used medicinally; a few can be used as spice plants, such as vanilla, etc.; most of them are brightly colored and have a long flowering period, and can be used as valuable ornamental plants with high commercial value. [0003] Among the 1300 species of endangered plants listed in my country's key protection, orchids account for more than ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02
Inventor 何明高宋松泉宋希强王瑞霞
Owner INST OF BOTANY CHINESE ACAD OF SCI
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