Method for tissue culture fast propagation of bletilla and application

A technology of bletilla striata and bletilla striata, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of low survival rate of transplanted seedlings in tissue culture, low natural germination rate, slow propagation speed, etc., and achieve rapid adaptation to natural The environment, the method is simple, and the effect of high application value

Inactive Publication Date: 2016-10-26
丽江海贝瑞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sustainable utilization of Bletilla striata has attracted people's attention, but the natural germination rate of Bletilla striata seeds is low, and currently it is mainly propagated by branching, with low reproduction rate and slow propagation speed; each gourd of Bletilla striata has tens of thousands of seeds, and seeds are used for breeding Tissue culture and rapid propagation, which can reproduce a large number of seedlings in a short period of time, is the only way to solve the problem of Bletilla striata seedlings. However, the cultivation technology of tissue cultured seedlings is not yet mature, and the survival rate of tissue cultured seedlings is low and the cycle is long. By understanding the natural germination and growth characteristics of Bletilla striata, it is the key to solve this bottleneck by researching and formulating tissue culture seedlings and transplanting techniques.

Method used

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  • Method for tissue culture fast propagation of bletilla and application
  • Method for tissue culture fast propagation of bletilla and application
  • Method for tissue culture fast propagation of bletilla and application

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Experimental program
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Effect test

Embodiment 1

[0030] In order to achieve the above object, the present invention adopts the following technical solutions:

[0031] A bletilla striata tissue culture rapid propagation method specifically comprises the following steps:

[0032] S1. Explant sterilization method: first wash the capsule with running water for 26 minutes, scrub the surface of the capsule with washing powder water, rinse twice with distilled water, disinfect with 75% alcohol for 2 minutes on the ultra-clean workbench, and then use 10% sodium hypochlorite solution Soak for 8 minutes, shake continuously during the period to make it thoroughly sterilized, rinse with sterile water 4 times, and dry the surface moisture with sterile filter paper for later use;

[0033] S2. Cut open the capsule under aseptic conditions, grab the capsule shell with tweezers, and shake the seeds gently onto the surface of the culture medium;

[0034] S3. Aseptic germination of Bletilla striata seeds: except for the special instructions o...

Embodiment 2

[0049] In order to achieve the above object, the present invention adopts the following technical solutions:

[0050] A bletilla striata tissue culture rapid propagation method specifically comprises the following steps:

[0051] S1. Explant sterilization method: first wash the capsule with running water for 28 minutes, scrub the surface of the capsule with washing powder water, rinse 3 times with distilled water, disinfect with 75% alcohol for 3 minutes on the ultra-clean workbench, and then use 10% sodium hypochlorite solution Soak for 10 minutes, shake continuously during the period to make it thoroughly sterilized, rinse with sterile water 5 times, and dry the surface moisture with sterile filter paper for later use;

[0052] S2. Cut open the capsule under aseptic conditions, grab the capsule shell with tweezers, and shake the seeds gently onto the surface of the culture medium;

[0053] S3. Aseptic germination of Bletilla striata seeds: except for the special instruction...

Embodiment 3

[0075] In order to achieve the above object, the present invention adopts the following technical solutions:

[0076] A bletilla striata tissue culture rapid propagation method specifically comprises the following steps:

[0077] S1. Explant sterilization method: first wash the capsule with running water for 32 minutes, scrub the surface of the capsule with washing powder water, rinse 3 times with distilled water, disinfect with 75% alcohol for 3 minutes on the ultra-clean workbench, and then use 10% sodium hypochlorite solution Soak for 12 minutes, shake continuously during the period to make it thoroughly sterilized, rinse with sterile water 6 times, and dry the surface moisture with sterile filter paper for later use;

[0078] S2. Cut open the capsule under aseptic conditions, grab the capsule shell with tweezers, and shake the seeds gently onto the surface of the culture medium;

[0079] S3. Aseptic germination of Bletilla striata seeds: except for the special instruction...

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Abstract

The invention discloses a method for tissue culture fast propagation of bletilla. The method specifically comprises the following steps of sterilization of an explant, sterile germination of bletilla seeds, observation of seed embryo form and seed coat breaking process, tissue culture fast propagation of bletilla, and determining of bletilla seed activity by a TTC (triphenyltetrazolium chloride) method. The method has the advantages that the method of obtaining bletilla seedlings is simple, and the seedling culture cost is greatly reduced; the germination is quick, the proliferation rate is high, the method is simple, the adding of tissue culture equipment is not needed, the production cost is low, the batched production is realized, and the application value is high.

Description

technical field [0001] The invention relates to the technical field of planting Chinese medicinal materials, and more specifically relates to a method for tissue culture and rapid propagation of bletilla striata. At the same time, the present invention also relates to the application of bletilla striata anti-oxidation. Background technique [0002] Bletilla striata, a species of the genus Bletilla striata, is a perennial herb with high ornamental value; its dried pseudobulb is a folk traditional Chinese medicinal material with high medicinal value, and its taste is bitter, sweet and astringent , cool in nature, returns lung, stomach, liver meridian. Function astringent to stop bleeding, reduce swelling and promote granulation, and mainly treat lung and stomach bleeding, traumatic bleeding, carbuncle sores, cracked skin, water and fire burns, etc. The higher ornamental value and extremely high medicinal value of Bletilla striata have led to a continuous increase in market d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01C1/02A61K36/898A61P39/06
CPCA01C1/025A01H4/008A61K36/898
Inventor 黄帅
Owner 丽江海贝瑞生物科技有限公司
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