Method for quickly detecting vigor of morchella esculenta L. hyphae

A technology of hickory chick and mycelium, which is applied in the field of physiologically active substance detection, can solve problems such as inability to completely stabilize inheritance of good traits, easy aging or degradation, time-consuming and labor-intensive problems, etc., to achieve stable and reliable measurement results, simple measurement of absorbance value, and experimental low cost effect

Inactive Publication Date: 2015-10-21
辽宁省农业科学院蔬菜研究所
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Problems solved by technology

[0004] Morel strains, like other edible and medicinal fungi, belong to fungi, with significant genetic variation, excellent traits cannot be completely inherited stably, and are prone to aging or degeneration, which brings great risks to batch cultivation and is also a difficult problem
[0005] How to select an excellent production species is th

Method used

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  • Method for quickly detecting vigor of morchella esculenta L. hyphae
  • Method for quickly detecting vigor of morchella esculenta L. hyphae
  • Method for quickly detecting vigor of morchella esculenta L. hyphae

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Embodiment

[0029] Embodiment A kind of method of rapid detection hickory chick mycelia vigor

[0030] (1) Test strains: the test strains used are shown in Table 1.

[0031] Table 1 Tested strains

[0032]

[0033] (2) Reagents:

[0034] Tris-HCl buffer: pH8.4;

[0035] TTC solution: 0.5% concentration by weight; prepared as needed.

[0036] Physiological saline: the weight percent concentration is 0.85%;

[0037] Mother seed plate medium: by weight percentage, peeled potato 20%, glucose 2%, peptone 0.3%, KH 2 PO 4 0.3%, MgSO 4 ·7H 2 O 0.15%, pH 7, balance water.

[0038] Liquid submerged medium: by weight percentage, peeled potato 20%, glucose 2%, peptone 0.3%, KH 2 PO 4 0.3%, MgSO 4 ·7H 2 O 0.15%, Vitamin B 1 10mg / L, water balance.

[0039] (3) The method for rapid detection of hickory chick mycelium vigor

[0040] 1) Mycelia culture

[0041] The strain to be tested was cultured at 23°C for 10 days in the mother plate culture medium, and then 2 pieces of bacterial b...

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Abstract

The invention relates to a method for quickly detecting the vigor of morchella esculenta L. hyphae. The method includes the steps that after a bacterial strain to be detected is cultivated in mother strain flat plate culture media, a bacterium block on a flat plate is cut to be transferred into liquid deep layer culture media for shake culture, and accordingly bacterium liquid is obtained; the bacterium liquid is collected into a centrifuge tube for centrifuging, liquid supernatant is removed, the hyphae are extracted, and the hyphae are washed by normal saline; the hyphae are weighed and added with water to be matched into the bacterium liquid to be detected; the bacterium liquid to be detected, a Tris-HCl buffer solution and a TTC (2,3,5-triphenyltetrazolium chloride) solution are transferred into the centrifuge tube for reacting; after reacting is completed, reacting liquid is placed on ice immediately, centrifuging is conducted at the temperature of 4 DEG C, a cell sediment is obtained, and an extraction agent is added into the centrifuged cell sediment so that extract liquor can be obtained; the extract liquor is centrifuged, the liquid supernatant is collected, and the absorbance value is detected at the wave length of 485 nm. Compared with a traditional method that the vigor of strains is detected according to hypha characteristics, the method has the advantages of being stable and reliable in detection result, good in reproducibility, high in accuracy, convenient to operate, high in speed and the like.

Description

technical field [0001] The invention relates to a method for detecting enzyme activity, more specifically a method for detecting the mycelia activity of edible and medicinal fungi, and belongs to the field of detection of physiologically active substances. Background technique [0002] Morel (Morchella esculenta L.), also known as delicious Morchella, commonly known as goat sparrow, corn fungus, etc., belongs to Ascomycotina (Ascomycotina), Discomycetes (Discomycetes) and Discomycetes ( Pezizales), Morchellaceae, Morchella. It gets its name because its cap is a mesh body full of depressions and ridges, shaped like a morel. Morchella has high nutritional value and medicinal value, and is a large edible and medicinal fungus. According to the determination, the protein content of morel is 22.1%, containing 19 kinds of amino acids, zinc, selenium, iron, germanium, copper and other trace elements and vitamin B 1 , B 2 , B 12 , niacin, pantothenic acid, biotin and other vitam...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 李红李超刘娜李宏亮何雨赵春燕
Owner 辽宁省农业科学院蔬菜研究所
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