79 results about "Normal saline flush" patented technology

A process for preparing the dried active amnion includes such steps as choosing health placenta tissue, sterilizing, separating amnion, removing sponge layer, spreading it on nitro-cellular paper, shearing by needed sizes, incubating in trypsase solution and/or EDTA solution, removing epithelial cells, flushing by physiologic saline, vacuum freeze drying, vacuum packing and gamma-ray radiating.

The invention discloses a formaldehyde-free animal specimen fixed liquid and a preparation method thereof, the formaldehyde-free animal specimen fixed liquid includes the following components: a biocide mildewcide, a protein denaturant, a polyol moisturizing agent and balance of water, and if the biocide mildewcide is in solid state, the mass concentration of the biocide mildewcide is 5-20 g/L, if the biocide mildewcide is liquid, the volume concentration of the biocide mildewcide is 1-2%; the volume concentration of the protein denaturant is 5%-40%; and the volume concentration of the polyol moisturizing agent is 2% to 10%. The formaldehyde-free animal specimen fixed liquid does not contain formaldehyde, and is non-volatile, and harmless to the human body; a fresh animal organ is washed with normal saline, and stored in the fixed liquid, a year later, the organ is not corrupted, free of mildew spot, and free of obvious color and shape change, the fixed liquid color has no obvious change, and the storage effect is good.

The invention provides a flushing fluid for surgery, which comprises solute and solvent, wherein the solute is one or two selected from cellulose glycollic ether, carboxymethyl chitosan or carboxymethyl chitosan, the solvent being water for injection, physiological saline solution, sodium chloride flushing fluid, mannitol flushing fluid, glucose flushing fluid, chlorhexidine flushing fluid, gentamicin physiological saline flushing fluid, active iodine flushing fluid, benzalkonium bromide flushing fluid, aminoacetic acid flushing fluid, metronidazole flushing fluid or physiological equilibrium liquid comprising phosphates cushioning liquid, NaHCO3, sodium gluconate and mannitol.

The invention relates to a method for extracting sub-totipotent stem cells from a chorion of a fetal surface of a placenta. The method comprises the steps of removing an amnion and stagnated blood from the placenta, and then repetitively washing the surface of the placenta to perform sterilization; shearing the chorion of the fetal surface in a glass utensil, removing placenta lobule tissues remained on the surface as much as possible, and shearing the chorion into small blocks; washing small tissue blocks by using a screen and a great amount of normal saline, and removing residual blood cells; performing tissue digestion: performing oscillatory digestion in a constant temperature shaker by using mixed enzymes; adding a proper amount of FBS for termination after digestion, performing filtration through a filter screen, and adding a great amount of normal saline to wash filter residues to obtain cells as many as possible; performing centrifugation, abandoning supernatant, adding saline water for washing and performing centrifugation to obtain mononuclear cells; performing magnetic bead sorting (OCT-4 positive, Nanog positive and STRO-1 negative) to obtain target cells. The invention further relates to the sub-totipotent stem cells obtained by adopting the method and pharmaceutical use thereof. The sub-totipotent stem cells have excellent characteristics.

The preparation process of heterogeneous bone transplanting material includes the following steps: 1. washing animal bone as the material with high pressure pulse distilled water or normal saline at normal pressure and room temperature and in aseptic condition to eliminate bone marrow and blood cell; 2. defatting with 95-100 wt% concentration ether solution and rinsing with aseptic water to eliminate ether; 3. ultralow temperature freezing in liquid nitrogen at normal pressure; and 4. irradiating with gamma ray.

The invention relates to the field of medicines, and particularly relates to an operation irrigating solution and a preparation method thereof. The irrigating solution comprises sodium hyaluronate and a solvent. The irrigating solution is characterized in that the solvent is normal saline, injection water, a ringer solution irrigating solution, a glucose irrigating solution, a mannitol irrigating solution, a chlorhexidine irrigating solution, a gentamycin normal saline irrigating solution, a povidone iodine irrigating solution, a bromogeramine irrigating solution, a glycine irrigating solution, a metronidazole irrigating solution or a physiological balanced solution; and the concentration of the sodium hyaluronate in the operation irrigating solution is 0.1-10g/L. The operation irrigating solution has the effects of operation irrigation, has functions of preventing adhesion and promoting wound healing after operation, and has the advantages of no toxic or side effect, good stability and excellent effect.

The invention relates to a method for detecting the total number of bacterial colonies in an activated lactobacillus drink, relating to a microorganism detecting method. The method comprises the following steps of: filtering diluent of the activated lactobacillus drink with a filter membrane, flushing the filter membrane with physiological saline, pasting the flushed filter membrane on a TTC nutrient agaragar tablet with the filtering face facing upwards, converting the nutrient agaragar tablet and culturing at 37 DEG C, and counting after culturing for 48+/-2h. By adopting the method, the total number of pollution bacterial colonies in the activated lactobacillus drink can be accurately, simply and quickly detected so as to provide an effective method for detecting the sanitation quality of the of the activated lactobacillus drink.

The invention relates to a preparation method of a biological wound protecting film. The preparation method comprises the steps of (1) selecting a placenta, and taking down the caul; (2) washing to remove the chorion to obtain the amnion; (3) washing the amnion by normal saline, and soaking; (4) scrapping the amnion to remove the residual chorion and the appendage of the chorion; (5) washing; (6) putting the amnion into soak solution, treating and then fixing the amnion on a special diaphragm for standing; (7) washing the amnion by normal saline, and then washing the amnion by protecting liquid; (8) putting the amnion into clean protecting liquid, and irradiating by an ultraviolet lamp; (9) putting the amnion into a packaging bag, injecting the protecting liquid into the packaging bag and sealing to obtain the biological wound protecting film. The wound protecting film has the characteristics of being good in toughness, elasticity and air permeability, and the like; furthermore, the wound is not exclusive with the biological wound protecting film, and the biological wound protecting film is strong in application property; according to the wound protecting film and the preparation method of the wound protecting film, the cost is low, so that the financial burden and the pain of a patient are relieved, and dressing change and excision of eschar do not need to be carried out on a patient who has burn within three degrees; therefore, the biological wound protecting film is a most advanced biotechnology in China at present.

The invention discloses a culture method of oral squamous cell carcinoma organoid. The culture method comprises the following steps: S1, preparing a dissociation enzyme; S2, preparing a culture medium, wherein the culture medium is composed of a basal culture medium DMEM/F12, an R-spondin1 conditional culture medium, a Wnt3A conditional culture medium, sterile water and functional components; S3, obtaining a sample through clinical operation, namely obtaining a tumor specimen through material taking or surgical resection, cutting tissue, washing the tissue with sterile normal saline for three times, soaking the tissue in a DMEM medium for 1 hour and continuing to dissociate; S4, tissue pretreatment, namely placing the sample obtained in the step S3 in a sterile 6-well plate, and cutting up sample tissue blocks by using a disposable sterile surgical blade; S5, tissue dissociation and digestion, namely adding the dissociation enzyme prepared in the step S1, repeating the process of centrifugation-supernatant removal for three times so as to fully remove the dissociation enzyme, and mixing the cells with the sample tissue blocks before the last time of centrifugation; and S6, carrying out organoid culture.

The invention discloses a biological adhesive which is characterized in that the adhesive comprises two parts which are solute and solvent; the solute is an azide benzoylate acidylated biomacromolecule compound; the solvent can be water, water for injection, normal saline, washing liquor of Ringer solution, washing liquor of mannitol, washing liquor of dextrose, washing liquor of chlorhexidine, washing liquor of gentamicin normal saline, washing liquor of glycin, washing liquor of metronidazole or physiologic balance solution; the weight percentage concentration of the azide benzoylate acidylated biomacromolecule compound in the biological adhesive is 0.1 percent to 20 percent. When in use, the biological adhesive needs to be solidified by using ultraviolet irradiation. The biological adhesive of the invention can be used for bonding active physiological tissues, wadding and patching physiological channels, sealing surface of wound to stanch bleeding, bonding physiological materials with non-physiological materials as well as bonding non-physiological materials, with short clotting time.

The invention discloses a guide wire seat. The guide wire seat comprises a first seat body and a second seat body. Through the cooperation of the first seat body and the second seat body, a guide wireis locked/loosened, and heparinized normal saline can be injected into a catheter under the condition that the guide wire and the guide wire seat are not detached to flush the inner wall of the catheter and the surface of the guide wire, so that the operation process is simplified, the operation time is shortened, and the pain of a patient is relieved.

The invention belongs to the technical field of biological proteins, and particularly relates to a method for purifying and preparing superoxide dismutase in rabbit blood. The method mainly includes technological steps of adding anticoagulants into the rabbit blood and centrifugally collecting precipitates; washing the precipitates by the aid of normal saline, centrifuging the precipitates and removing hemoglobin; stirring and dissolving the blood by the aid of deionized water with equal volumes; adding 95% of precooled ethyl alcohol with the equal volumes and precooled chloroform with 0.2-times volume into the dissolved blood; stirring and centrifuging the blood and acquiring supernatants; heating the supernatants until the temperatures of the supernatants reach 60 DEG C, preserving heat for 15 minutes, quickly cooling the supernatants until the temperatures of the supernatants reach the room temperature, centrifuging the supernatants and acquiring second supernatants; cooling the second supernatants in normal saline, adding precooled acetone into the second supernatants, stirring the second supernatants, acquiring second precipitates and dissolving the second precipitates by the aid of deionized water; carrying out desalination and chromatography on the second precipitates; washing the second precipitates twice by the aid of HAC-NaAC liquor, then dissolving the second precipitates again by the aid of PBS liquor and filtering the second precipitates by the aid of 0.45 micrometer filter paper; carrying out ion exchange chromatography on the second precipitates; carrying out ultra-filtration on the second precipitates; centrifuging SOD eluent under the condition of temperature of 4 DEG C, acquiring third supernatants which are SOD stock liquid. The method has the advantages that the method is easy to implement, and the superoxide dismutase extracted by the aid of the method is high in purity.