79 results about "Normal saline flush" patented technology

The invention discloses a preparation method of an immobilized microorganism embedding microsphere for restoring a riverbed bottom mud ecosystem. The preparation method comprises the steps of PVA (polyvinyl alcohol) gel preparation, cross-linking agent preparation and microsphere preparation, wherein the PVA gel preparation comprises the steps of preparing a PVA solution with a concentration of 8-10%, adding embedding agents to the PVA solution, namely sodium alginate with a concentration of 0.5-2%, calcium carbonate with a concentration of 0.2-0.5%, silicon dioxide with a concentration of 2.0-4.0%, and 300-500 mesh attapulgite powder or 300-500 mesh activated carbon powder with a concentration of 0.5-1.0%, and adding activated limon microorganism bacterium liquid with a concentration of 10-15%; and the microsphere preparation comprises the steps of dropwise adding PVA gel to a calcium chloride saturated boric acid solution, stirring, obtaining immobilized microorganism activated spherular particles with the particle size of 3-5mm, conducting immobilized crosslinking for 24-36h at 4-8 DEG C, taking out, and washing with normal saline. Compared with the prior art, the microsphere can remove total organic carbon and total nitrogen in riverbed bottom mud effectively, and can improve COD (chemical oxygen demand), ammonia nitrogen and total nitrogen pollution conditions of an overlying water body of the bottom mud, and the preparation method is an ecological riverway management scheme which is efficient, low-consumption and simple to operate, and has a development prospect.

The invention relates to a biocompatible pre-gelatinized modified starch. The water absorbency is not less than one time, and the biocompatible pre-gelatinized modified starch is taken as biocompatible hemostatic material, biocompatible anti-blocking material, biocompatible tissue-healing promoting material, biocompatible surgical sealant or biocompatible wound closure tissue glue. The invention has the advantages that the biocompatible pre-gelatinized modified starch is directly acted on the wounded area with blood for immediately stopping bleeding, has obviously increased water absorbency and speed of water absorption and greater viscosity and stickiness and further plays the role in preventing the tissue and the blood vessel from being damaged during the process of stopping bleeding; the modified starch is easy to swell or dissolve in water, and is washed by normal saline after the bleeding stopping so as to reduce the residual in the body, to be favorable for wound healing and to avoid the pain due to tearing the gauze and the bandage out; the pre-gelatinized modified starch has the actions of bacterial resistance and anti-inflammatory; and the pre-gelatinized modified starch is stable, not easy to decompose, long in guarantee period, convenient for storage, resistant at high pressure and low pressure, resistant at high temperature and low temperature and not easy to change the physicochemical characteristics.

A process for preparing the dried active amnion includes such steps as choosing health placenta tissue, sterilizing, separating amnion, removing sponge layer, spreading it on nitro-cellular paper, shearing by needed sizes, incubating in trypsase solution and / or EDTA solution, removing epithelial cells, flushing by physiologic saline, vacuum freeze drying, vacuum packing and gamma-ray radiating.

The invention discloses a formaldehyde-free animal specimen fixed liquid and a preparation method thereof, the formaldehyde-free animal specimen fixed liquid includes the following components: a biocide mildewcide, a protein denaturant, a polyol moisturizing agent and balance of water, and if the biocide mildewcide is in solid state, the mass concentration of the biocide mildewcide is 5-20 g / L, if the biocide mildewcide is liquid, the volume concentration of the biocide mildewcide is 1-2%; the volume concentration of the protein denaturant is 5%-40%; and the volume concentration of the polyol moisturizing agent is 2% to 10%. The formaldehyde-free animal specimen fixed liquid does not contain formaldehyde, and is non-volatile, and harmless to the human body; a fresh animal organ is washed with normal saline, and stored in the fixed liquid, a year later, the organ is not corrupted, free of mildew spot, and free of obvious color and shape change, the fixed liquid color has no obvious change, and the storage effect is good.

The invention relates to a manufacture method of a tissue engineering bracket with both an internal microstructure and an individualized appearance. The manufacture method comprises the following steps of: (1) designing the negative shape of a bracket structure; (2) printing the negative shape of the porous-structure bracket by a three-dimensional paraffin type printer; (3) uniformly mixing a biological material with self-solidifying property or hot coagulation property into slurry shape with a solution such as normal saline, pouring the slurry into holes of the negative shape of the porous-structure bracket, cooling and solidifying, and scraping off the redundant biological material from the surface of the bracket; and (4) heating the poured negative shape of the bracket into a heating furnace till the temperature of the negative shape is higher than the temperature of the melting point of the paraffin shape of the bracket, preserving the temperature for 1-5 minutes till paraffin is melted and disappears and the biological material is solidified to obtain the biological material bracket, and washing the biological material bracket with normal saline so as to obtain the tissue engineering bracket with both the internal microstructure and the individualized appearance. The manufacture method has wider adaptability to biological materials and internal microstructure controllability and can be used for manufacturing the tissue engineering bracket with the internal microstructure and the individualized appearance.

The invention provides a flushing fluid for surgery, which comprises solute and solvent, wherein the solute is one or two selected from cellulose glycollic ether, carboxymethyl chitosan or carboxymethyl chitosan, the solvent being water for injection, physiological saline solution, sodium chloride flushing fluid, mannitol flushing fluid, glucose flushing fluid, chlorhexidine flushing fluid, gentamicin physiological saline flushing fluid, active iodine flushing fluid, benzalkonium bromide flushing fluid, aminoacetic acid flushing fluid, metronidazole flushing fluid or physiological equilibrium liquid comprising phosphates cushioning liquid, NaHCO3, sodium gluconate and mannitol.

The invention relates to a method for extracting sub-totipotent stem cells from a chorion of a fetal surface of a placenta. The method comprises the steps of removing an amnion and stagnated blood from the placenta, and then repetitively washing the surface of the placenta to perform sterilization; shearing the chorion of the fetal surface in a glass utensil, removing placenta lobule tissues remained on the surface as much as possible, and shearing the chorion into small blocks; washing small tissue blocks by using a screen and a great amount of normal saline, and removing residual blood cells; performing tissue digestion: performing oscillatory digestion in a constant temperature shaker by using mixed enzymes; adding a proper amount of FBS for termination after digestion, performing filtration through a filter screen, and adding a great amount of normal saline to wash filter residues to obtain cells as many as possible; performing centrifugation, abandoning supernatant, adding saline water for washing and performing centrifugation to obtain mononuclear cells; performing magnetic bead sorting (OCT-4 positive, Nanog positive and STRO-1 negative) to obtain target cells. The invention further relates to the sub-totipotent stem cells obtained by adopting the method and pharmaceutical use thereof. The sub-totipotent stem cells have excellent characteristics.

The invention pertains to the field of biomedical technology, in particular to an orthotopic transplanted mouse liver cancer model which takes the research of the occurrence and development mechanisms of liver cancer and the development of anti-tumor drugs as the purposes, and the preparation method and the application thereof. The H22 cell strain of stable transfection EGFP is injected into the BALB / c mouse peritoneal cavity for amplification; the ascites is extracted from the H22 ascites cancer mouse peritoneal cavity, then cell separation is carried out and cell suspension is prepared; the mouse peritoneal cavity is opened, and the cell suspension is extracted by a micro-syringe to be injected into the mouse liver; a cotton bud with 75 percent alcohol is pressed on the needle hole till the surface of the liver does not bleed any more, and the needle hole is sealed by bonding agent; then the liver surface is washed by physiological saline, and layer separation is carried out and the peritoneal cavity is closed. The results show that the occurrence rate of mouse liver cancer is 100 percent and the natural remission rate is zero. The model is an ideal orthotopic liver cancer model which can be used in the research of the development and metastasis mechanisms of orthotopic liver cancer and can also be applicable to the development of anti-tumor drugs, the experimental treatment and diagnosis research of liver cancer.

The invention relates to a method to prepare and preserve homogeneous bioactive bone tissue, its steps: 1, obtaining: on the aseptic condition, obtaining homogeneous bone tissue; 2, finishing: eliminating soft tissue and periosteum, eliminating bone marrow tissue, and reserving partial joint bursa, ligament and adhesive point of muscle; 3, defatting: dipping in chloroformic methanol, washing with physiological saline and then packaging; 4, gradient temperature reducing: placing the preprocessed bone tissue in program temperature reducer to make gradient temperature reduction up to -40 deg.C--60 deg.C for 20-80 min.; 5, preserving: putting the bone tissue in the -60 deg.C - 80 deg. C refrigerator. It can keep the integrality of bone ultramicro structure, bone inducing activity, bioforce intensity and maintain a certain quantity of survival soft bone cells.

The invention relates to the field of medicines, and particularly relates to an operation irrigating solution and a preparation method thereof. The irrigating solution comprises sodium hyaluronate and a solvent. The irrigating solution is characterized in that the solvent is normal saline, injection water, a ringer solution irrigating solution, a glucose irrigating solution, a mannitol irrigating solution, a chlorhexidine irrigating solution, a gentamycin normal saline irrigating solution, a povidone iodine irrigating solution, a bromogeramine irrigating solution, a glycine irrigating solution, a metronidazole irrigating solution or a physiological balanced solution; and the concentration of the sodium hyaluronate in the operation irrigating solution is 0.1-10g / L. The operation irrigating solution has the effects of operation irrigation, has functions of preventing adhesion and promoting wound healing after operation, and has the advantages of no toxic or side effect, good stability and excellent effect.

The invention relates to a method for detecting the total number of bacterial colonies in an activated lactobacillus drink, relating to a microorganism detecting method. The method comprises the following steps of: filtering diluent of the activated lactobacillus drink with a filter membrane, flushing the filter membrane with physiological saline, pasting the flushed filter membrane on a TTC nutrient agaragar tablet with the filtering face facing upwards, converting the nutrient agaragar tablet and culturing at 37 DEG C, and counting after culturing for 48+ / -2h. By adopting the method, the total number of pollution bacterial colonies in the activated lactobacillus drink can be accurately, simply and quickly detected so as to provide an effective method for detecting the sanitation quality of the of the activated lactobacillus drink.

The invention relates to a preparation method of a biological wound protecting film. The preparation method comprises the steps of (1) selecting a placenta, and taking down the caul; (2) washing to remove the chorion to obtain the amnion; (3) washing the amnion by normal saline, and soaking; (4) scrapping the amnion to remove the residual chorion and the appendage of the chorion; (5) washing; (6) putting the amnion into soak solution, treating and then fixing the amnion on a special diaphragm for standing; (7) washing the amnion by normal saline, and then washing the amnion by protecting liquid; (8) putting the amnion into clean protecting liquid, and irradiating by an ultraviolet lamp; (9) putting the amnion into a packaging bag, injecting the protecting liquid into the packaging bag and sealing to obtain the biological wound protecting film. The wound protecting film has the characteristics of being good in toughness, elasticity and air permeability, and the like; furthermore, the wound is not exclusive with the biological wound protecting film, and the biological wound protecting film is strong in application property; according to the wound protecting film and the preparation method of the wound protecting film, the cost is low, so that the financial burden and the pain of a patient are relieved, and dressing change and excision of eschar do not need to be carried out on a patient who has burn within three degrees; therefore, the biological wound protecting film is a most advanced biotechnology in China at present.

The invention belongs to the technical field of biological product separation culture, and particularly relates to a human umbilical cord mesenchymal stem cell separation culture method. The method comprises the following steps that an in-vitro umbilical cord of a term fetus born by caesarean delivery is taken and put into a sterile tissue preserving fluid for storage; flushing is conducted multiple times with normal saline, and remained bloodstains are removed; two umbilical veins and two umbilical arteries of tissue mass are removed with toothed tweezers, and Wharton's jelly is completely cut into pieces with sterile scissors; the Wharton's jelly which is cut into pieces is transferred into a culture medium, and vibration and centrifugation are conducted in sequence; the centrifugal sedimentation part is transferred into a cell culture flask; after culture is conducted for 5-6 days, it can be seen that part of cells climb out from the periphery of small pieces of the tissue, then a culture substrate is replaced once every three days, and culture continuous to be conducted; on the fourteenth day or so, the degree of cell fusion reaches 80% or above, and spiral growth is achieved;afterwards, each transmission of the next generation takes three days, and adequate mesenchymal stem cells can be obtained. Compared with a traditional tissue method, the method is simpler, and the purity and yield of stem cells are higher.

The invention discloses a culture method of oral squamous cell carcinoma organoid. The culture method comprises the following steps: S1, preparing a dissociation enzyme; S2, preparing a culture medium, wherein the culture medium is composed of a basal culture medium DMEM / F12, an R-spondin1 conditional culture medium, a Wnt3A conditional culture medium, sterile water and functional components; S3, obtaining a sample through clinical operation, namely obtaining a tumor specimen through material taking or surgical resection, cutting tissue, washing the tissue with sterile normal saline for three times, soaking the tissue in a DMEM medium for 1 hour and continuing to dissociate; S4, tissue pretreatment, namely placing the sample obtained in the step S3 in a sterile 6-well plate, and cutting up sample tissue blocks by using a disposable sterile surgical blade; S5, tissue dissociation and digestion, namely adding the dissociation enzyme prepared in the step S1, repeating the process of centrifugation-supernatant removal for three times so as to fully remove the dissociation enzyme, and mixing the cells with the sample tissue blocks before the last time of centrifugation; and S6, carrying out organoid culture.

The invention discloses a manufacturing method of dry fruit powder of medlar. The method comprises the steps of cleaning fresh medlar fruits with clear water, then rinsing and draining, then putting in a ClO2 solution so as to be soaked, flushing, draining, putting in a sodium percarbonate solution so as to be soaked, then flushing with purified water and removing pedicel, flushing with normal saline, then rinsing with purified water and then draining, pre-freezing dry medlar fruits after draining, performing vacuum freeze drying, performing vacuum drying, grinding into powder, and packaging to enter a warehouse. The method has the advantages of simplicity in operation and short production cycle, and the obtained dry powder has the advantages of good color and high nutrition.

The invention discloses a culture method of trichoderma producing cellulase. The method includes: inoculating the trichoderma onto a PDA slant, culturing at 45 DEG C for 5 d, washing spores with 5 mL of sterilized normal saline after the spores are formed, counting the collected spore solution by using a blood counting chamber so as to allow the number of the spores in each milliliter of the solution to be 106-107, inoculating onto a solid fermentation culture medium according to a 5% inoculating amount, and culturing at 28 DEG C for 146 h. The enzyme output in unit volume in trichoderma solid-state fermentation is higher than that of liquid fermentation. The method has characteristics of no stirring, low energy consumption, low equipment investment, simple post treatment and basically no pollution.

The invention discloses a biological adhesive which is characterized in that the adhesive comprises two parts which are solute and solvent; the solute is an azide benzoylate acidylated biomacromolecule compound; the solvent can be water, water for injection, normal saline, washing liquor of Ringer solution, washing liquor of mannitol, washing liquor of dextrose, washing liquor of chlorhexidine, washing liquor of gentamicin normal saline, washing liquor of glycin, washing liquor of metronidazole or physiologic balance solution; the weight percentage concentration of the azide benzoylate acidylated biomacromolecule compound in the biological adhesive is 0.1 percent to 20 percent. When in use, the biological adhesive needs to be solidified by using ultraviolet irradiation. The biological adhesive of the invention can be used for bonding active physiological tissues, wadding and patching physiological channels, sealing surface of wound to stanch bleeding, bonding physiological materials with non-physiological materials as well as bonding non-physiological materials, with short clotting time.

The invention discloses a method for culturing stem cell factors for beauty. The method comprises the following steps of (1) placing mesenchymal stem cells in a serum-free medium, sub-culturing to 4 generations, flushing with normal saline, then adding digestive juice, and carrying out digestion counting; (2) when the fusion degree of the cells obtained in the step (1) reaches 80-90%, replacing with an induction culture medium, and continuing to culture; (3) filtering the induction culture medium obtained in the step (2) for 3-4 times, merging filtrates, and concentrating to 1 / 10-1 / 5 of the original volume; adding normal saline containing sodium citrate to complement to the original volume, filtering again, collecting filtrate, and concentrating the filtrate to 1 / 20-1 / 10 of the original volume; and (4) filtering the product obtained in the step (3) by using a filter membrane with the molecular weight cutoff of 8-14KD, and collecting to obtain the stem cell factor concentrated solution. According to the method, the content of cell factors in the prepared concentrated solution can be effectively increased.

The invention discloses a medical robot with a function of cleaning an arm wound before an operation. The medical robot comprises a main body, wherein sensors are symmetrically and fixedly arranged atthe upper and lower ends in the main body; a movable rod capable of moving back and forth is arranged in the main body and is positioned between the sensors at the upper and lower sides; a left sliding cavity is arranged between the sensors at the upper and lower sides and is positioned at the left side of the movable rod; and a left sliding plate is arranged in the left sliding cavity in a manner of sliding leftward and rightward. According to the medical robot disclosed by the invention, a pre-treatment manner of small and medium-size wounds of an arm is adopted; the medical robot is fixedon the arm through movable wheels and then is washed through normal saline to wash and disinfect the wounds; then body hairs nearby the wounds are removed through a knife net; finally, the wounds arewiped through sterile gauze and are prepared for the subsequent operation, so that secondary infection caused by the fact that the wounds are not properly treated before the operation is avoided; andmeanwhile, the operation efficiency is improved.

The invention relates to an animal eyeball pathological section manufacturing method which comprises the following steps: picking an animal eyeball, reserving 3-8mm optic nerve, and washing with normal saline to remove bloodstain; placing the animal eyeballs in normal saline, and then subjecting the animal eyeballs to heating treatment for 4-12 min with medium fire in a microwave oven; putting animal eyeballs into a stationary liquid prepared from glacial acetic acid, chloroform and methanol, and fixing for 60-90 hours; dehydrating the animal eyeballs 2-4 times by using n-butyl alcohol, wherein each time lasts for 0.5-3 hours; then, subjecting the animal eyeballs to transparent treatment with dimethylbenzene for 2-4 times, wherein each time lasts for 10-20 min, and the total time of dimethylbenzene transparent treatment is 30-50 min; performing the first paraffin treatment for 1-2 hours, performing the second paraffin treatment for 1-2 hours, and performing paraffin embedding after the treatment; and slicing according to a set thickness. When the animal eyeball pathological section is manufactured by the method, the eyeballs are treated by microwaves in combination with the structural characteristics of the animal eyeballs, so that the eyeball structural protein is denatured, the strength of the eyeball structural strength is pre-enhanced, and the method has important significance on accurate research and teaching of fine structures of the animal eyeballs.

The invention relates to a method for quickly detecting the vigor of morchella esculenta L. hyphae. The method includes the steps that after a bacterial strain to be detected is cultivated in mother strain flat plate culture media, a bacterium block on a flat plate is cut to be transferred into liquid deep layer culture media for shake culture, and accordingly bacterium liquid is obtained; the bacterium liquid is collected into a centrifuge tube for centrifuging, liquid supernatant is removed, the hyphae are extracted, and the hyphae are washed by normal saline; the hyphae are weighed and added with water to be matched into the bacterium liquid to be detected; the bacterium liquid to be detected, a Tris-HCl buffer solution and a TTC (2,3,5-triphenyltetrazolium chloride) solution are transferred into the centrifuge tube for reacting; after reacting is completed, reacting liquid is placed on ice immediately, centrifuging is conducted at the temperature of 4 DEG C, a cell sediment is obtained, and an extraction agent is added into the centrifuged cell sediment so that extract liquor can be obtained; the extract liquor is centrifuged, the liquid supernatant is collected, and the absorbance value is detected at the wave length of 485 nm. Compared with a traditional method that the vigor of strains is detected according to hypha characteristics, the method has the advantages of being stable and reliable in detection result, good in reproducibility, high in accuracy, convenient to operate, high in speed and the like.

The invention discloses an extraction and preparation method of placenta pluripotent stem cells. The extraction and preparation method comprises the following steps: under a sterile condition, obtaining placenta tissues; repeatedly washing with PBS, disinfecting with betadine and sufficiently washing a placenta with a saline solution for at least three times; cutting the placenta tissues into fragments with the size of 1*1*1mm<3> and putting the fragments into a sterile centrifugal tube; digesting the placenta tissue fragments with 0.25% trypsase and 0.2% IV type collagenase; centrifuging and collecting to obtain cell sediment; re-suspending the cell sediment into a fetal calf blood serum alpha-MEM culture medium with the volume percent of 10% and freezing by a conventional method; and carrying out flow cytometry analysis on CD73, CD90 and CD105 cell expression. The placenta is obtained under the sterile condition and the placenta is subjected to a series of pre-treatment including PBS repeated washing, placenta surface disinfection and washing of the placenta with the saline solution, so as to guarantee that the obtained placenta is fresh and conveniently guarantee the activity of the pluripotent stem cells in the placenta tissues.

The invention discloses a guide wire seat. The guide wire seat comprises a first seat body and a second seat body. Through the cooperation of the first seat body and the second seat body, a guide wireis locked / loosened, and heparinized normal saline can be injected into a catheter under the condition that the guide wire and the guide wire seat are not detached to flush the inner wall of the catheter and the surface of the guide wire, so that the operation process is simplified, the operation time is shortened, and the pain of a patient is relieved.

The invention belongs to the technical field of biological proteins, and particularly relates to a method for purifying and preparing superoxide dismutase in rabbit blood. The method mainly includes technological steps of adding anticoagulants into the rabbit blood and centrifugally collecting precipitates; washing the precipitates by the aid of normal saline, centrifuging the precipitates and removing hemoglobin; stirring and dissolving the blood by the aid of deionized water with equal volumes; adding 95% of precooled ethyl alcohol with the equal volumes and precooled chloroform with 0.2-times volume into the dissolved blood; stirring and centrifuging the blood and acquiring supernatants; heating the supernatants until the temperatures of the supernatants reach 60 DEG C, preserving heat for 15 minutes, quickly cooling the supernatants until the temperatures of the supernatants reach the room temperature, centrifuging the supernatants and acquiring second supernatants; cooling the second supernatants in normal saline, adding precooled acetone into the second supernatants, stirring the second supernatants, acquiring second precipitates and dissolving the second precipitates by the aid of deionized water; carrying out desalination and chromatography on the second precipitates; washing the second precipitates twice by the aid of HAC-NaAC liquor, then dissolving the second precipitates again by the aid of PBS liquor and filtering the second precipitates by the aid of 0.45 micrometer filter paper; carrying out ion exchange chromatography on the second precipitates; carrying out ultra-filtration on the second precipitates; centrifuging SOD eluent under the condition of temperature of 4 DEG C, acquiring third supernatants which are SOD stock liquid. The method has the advantages that the method is easy to implement, and the superoxide dismutase extracted by the aid of the method is high in purity.

The invention relates to a method for building mouse primary liver cancer mode to research liver cancer and anti-liver-cancer drug, wherein it comprises that: taking ascites from the Heps ascites carcinoma mouse abdominal cavity; separating cell and preparing cell suspension; opening mouse abdominal cavity; using injector to absorb cell suspension to be injected into mouse liver; using 75% alcohol cotton swab to press the needle hole until the liver does not blood; using alpha-cyanoacrylate adhesive to seal needle hole; using normal saline to wash the liver; closing abdominal cavity. The result has proved that: the liver cancer rate is 100%, the natural anneal rate is zero. And the inventive mode can research the primary liver cancer mechanism, etc.

The invention relates to a biocompatible pre-gelatinized modified starch. The water absorbency is not less than one time, and the biocompatible pre-gelatinized modified starch is taken as biocompatible hemostatic material, biocompatible anti-blocking material, biocompatible tissue-healing promoting material, biocompatible surgical sealant or biocompatible wound closure tissue glue. The invention has the advantages that the biocompatible pre-gelatinized modified starch is directly acted on the wounded area with blood for immediately stopping bleeding, has obviously increased water absorbency and speed of water absorption and greater viscosity and stickiness and further plays the role in preventing the tissue and the blood vessel from being damaged during the process of stopping bleeding; the modified starch is easy to swell or dissolve in water, and is washed by normal saline after the bleeding stopping so as to reduce the residual in the body, to be favorable for wound healing and to avoid the pain due to tearing the gauze and the bandage out; the pre-gelatinized modified starch has the actions of bacterial resistance and anti-inflammatory; and the pre-gelatinized modified starch is stable, not easy to decompose, long in guarantee period, convenient for storage, resistant at high pressure and low pressure, resistant at high temperature and low temperature and not easy to change the physicochemical characteristics.