Method for constructing primary liver cancer model of mice

Abstract

The invention relates to a method for building mouse primary liver cancer mode to research liver cancer and anti-liver-cancer drug, wherein it comprises that: taking ascites from the Heps ascites carcinoma mouse abdominal cavity; separating cell and preparing cell suspension; opening mouse abdominal cavity; using injector to absorb cell suspension to be injected into mouse liver; using 75% alcohol cotton swab to press the needle hole until the liver does not blood; using alpha-cyanoacrylate adhesive to seal needle hole; using normal saline to wash the liver; closing abdominal cavity. The result has proved that: the liver cancer rate is 100%, the natural anneal rate is zero. And the inventive mode can research the primary liver cancer mechanism, etc.

Description

1. Technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a method for establishing a mouse primary liver cancer model for the purpose of studying the mechanism of occurrence and development of liver cancer and the development of antitumor drugs. 2. Background technology [0002] Primary liver cancer is one of the common malignant tumors in my country, and its mortality rate is high, ranking third in the death order of malignant tumors. About 110,000 people die from liver cancer every year in my country, accounting for 45% of the death toll from liver cancer in the world. The treatment of liver cancer needs to be improved urgently. An ideal animal model of liver cancer is an indispensable experimental tool for the development of anti-tumor drugs, the mechanism of liver cancer development, diagnosis and treatment. Therefore, the successful establishment of experimental animal models of liver cancer plays a vital role i...

AI Technical Summary

Problems solved by technology

In the experiment of Han et al., the production procedure is cumbersome and the success rate is low, and the model needs to be subcutaneously implanted in mice to form solid tumors, so the tumor tissue blocks transplanted in each experiment cannot come from the same mouse body, and the cut tissue The size of the block is not easy to be consistent, the content is not uniform, and it contains other non-cancerous tissues, which will seriously affect the repeatability and reliability of the experiment, and cannot fully reflect the effect of anti-liver cancer drugs

Li Qi et al. and Shao Chengwei et al. established a rat transplanted liver cancer model by intrahepatic injection of Walker-256 cells. The Walker-256 cell line used in the model can better simulate the expansion and infiltration of human liver cancer after implantation into the liver. However, the cell line is derived from the spontaneous carcinosarcoma of Wistar rat mammary glands rather than liver cancer tissue. Therefore, this model cannot well simulate the occurrence and development of liver cancer, let alone meet the requirements of anti-liver cancer drug development and screening, liver cancer molecular biology research needs

In addition, rats are more expensive and require a larger breeding space, so it is not easy to produce in large quantities

Although the existing human-nude mouse xenograft liver cancer model has a high transplantation success rate, the immune system of nude mice is deficient, which cannot reflect the real situation of normal organisms, and it is impossible to study the role of the immune system in the occurrence of liver cancer and the The impact on the subsequent development of liver cancer cannot simulate the normal metabolism of drugs in the body

In addition, the nude mouse-human liver cancer transplantation model has high technical requirements, low postoperative survival rate, high sterility requirements, difficult feeding, and high price, so the practicability of this model is greatly limited.

Various existing animal models of liver cancer have certain defects and cannot meet the needs of the current biomedical field

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