Culture method of trichoderma producing cellulase

A cultivation method and cellulase technology, applied in the field of biological fermentation, can solve the problems of high energy consumption, low yield, a large amount of waste gas, waste water, etc., and achieve the effects of low energy consumption, low equipment investment, and simple post-treatment

Inactive Publication Date: 2014-06-25
QINGDAO ZHONGREN PHARMA
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Problems solved by technology

[0006] In order to overcome the above-mentioned defects that exist in the prior art, the object of the present invention is to provide a method for cultivating Trichoderma that produces cellulase, which solves the problem that the prior art liquid submerged fermentation Trichoderma will produce a large amount of waste gas, waste water, high energy consumption, The problem of low yield

Method used

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Embodiment

[0013] The culture method of the Trichoderma that produces cellulase of the present embodiment, solid fermentation culture, Trichoderma is inoculated on PDA inclined-plane 45 ℃ and cultivated 5d, after waiting to form spore, rinse spore with 5ml sterilized normal saline, collect The spore solution that arrives is counted with a hemocytometer, so that the content of spores in every milliliter of solution is within 10 6 one 10 7 One, inoculate in solid fermentation medium by 5% inoculum size, cultivate 146h under 28 ℃; Described solid fermentation medium is added a certain amount of basic salt solution by 5g corn stalks, 3.5g (NH ) 2 SO 4 , 3g KH 2 PO 4 , 0.5g MgSO 4 .7H 2 O, 0.5g CaCl 2 , 1000ml of distilled water is sterilized at 121°C for 20 minutes to configure.

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Abstract

The invention discloses a culture method of trichoderma producing cellulase. The method includes: inoculating the trichoderma onto a PDA slant, culturing at 45 DEG C for 5 d, washing spores with 5 mL of sterilized normal saline after the spores are formed, counting the collected spore solution by using a blood counting chamber so as to allow the number of the spores in each milliliter of the solution to be 106-107, inoculating onto a solid fermentation culture medium according to a 5% inoculating amount, and culturing at 28 DEG C for 146 h. The enzyme output in unit volume in trichoderma solid-state fermentation is higher than that of liquid fermentation. The method has characteristics of no stirring, low energy consumption, low equipment investment, simple post treatment and basically no pollution.

Description

[0001] technical field [0002] The invention belongs to the technical field of biological fermentation, and in particular relates to a method for cultivating cellulase-producing Trichoderma. [0003] Background technique [0004] The modern fermented Trichoderma industry is mainly based on liquid submerged fermentation, but submerged liquid fermentation will produce a large amount of fermentation wastewater and waste gas, and there is also the problem of high power consumption caused by mechanical agitation, and the liquid fermentation yield of Trichoderma is also very low. [0005] Contents of the invention [0006] In order to overcome the above-mentioned defects that exist in the prior art, the object of the present invention is to provide a method for cultivating Trichoderma that produces cellulase, which solves the problem that the prior art liquid submerged fermentation Trichoderma will produce a large amount of waste gas, waste water, high energy consumption, The...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/885
Inventor 张述智夏伟朱绍辉张浩王晓丽徐权汗李之详许团辉
Owner QINGDAO ZHONGREN PHARMA
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