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A kind of preparation method of probiotic microcapsule for external use

A probiotics and microcapsule technology, applied in the field of biomedicine, can solve the problems of unsatisfactory embedding rate and survival rate, single components, etc., and achieve the effects of prolonging the shelf life at room temperature, good encapsulation rate, and promoting regeneration.

Active Publication Date: 2018-05-04
威海美合生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its embedding rate and survival rate are not ideal, and its components are relatively simple.

Method used

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  • A kind of preparation method of probiotic microcapsule for external use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A preparation method of probiotic microcapsules for external use, the steps are as follows:

[0024] (1), inoculate the freeze-dried bacterial powders of Bifidobacterium, Lactobacillus acidophilus, and Lactobacillus rhamnosus in the Erlenmeyer flask for activation treatment respectively, that is, after cultivating at 30°C for 60 hours, expand the fermentation culture, when the fermentation liquid is active Bacterial concentration reaches 10 10 cfu / g, stop the fermentation, mix the fermentation liquid of the three bacteria according to the volume ratio of 3:3:4, and obtain the bacterial suspension;

[0025] (2) Take fresh kudzu rice, break it, irradiate it with microwave power 280W for 5 minutes, add 5 times the weight of water to boil for 20 minutes, filter while it is hot, add water to the drug residue and continue to decoct, repeat the extraction three times, and combine The filtrate is finely filtered to obtain the extract of Gexianmi;

[0026] (3) Mix the bacteria...

Embodiment 2

[0029] A preparation method of probiotic microcapsules for external use, the steps are as follows:

[0030] (1), inoculate the freeze-dried bacterial powders of Bifidobacterium, Lactobacillus acidophilus, and Lactobacillus rhamnosus in the Erlenmeyer flask for activation treatment respectively, that is, after cultivating at 35°C for 50 hours, expand the fermentation culture, when the fermentation liquid is active Bacterial concentration reaches 10 10 cfu / g, stop the fermentation, mix the fermentation liquid of the three bacteria according to the volume ratio of 3:3:4, and obtain the bacterial suspension;

[0031] (2) Take fresh kudzu rice, break it, irradiate it with microwave power 320W for 3 minutes, add 5 times the weight of water to boil for 25 minutes, filter while it is hot, add water to the drug residue and continue to decoct, repeat the extraction three times, and combine The filtrate is finely filtered to obtain the extract of Gexianmi;

[0032](3) Mix the bacterial...

Embodiment 3

[0035] A preparation method of probiotic microcapsules for external use, the steps are as follows:

[0036] (1), inoculate the freeze-dried bacterial powders of Bifidobacterium, Lactobacillus acidophilus, and Lactobacillus rhamnosus in the Erlenmeyer flask for activation treatment respectively, that is, after cultivating at 33°C for 59 hours, expand the fermentation culture, when the fermentation liquid is active Bacterial concentration reaches 10 10 cfu / g, stop the fermentation, mix the fermentation liquid of the three bacteria according to the volume ratio of 3:3:4, and obtain the bacterial suspension;

[0037] (2) Take fresh kudzu rice, break it, irradiate it with microwave power 300W for 4 minutes, add 5 times the weight of water to boil for 21 minutes, filter while it is hot, add water to the drug residue and continue to decoct, repeat the extraction three times, and combine The filtrate is finely filtered to obtain the extract of Gexianmi;

[0038] (3) Mix the bacteria...

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Abstract

The invention discloses an external probiotics microcapsule preparation method. By making full use of the synergistic effect of plant extracts and probiotics, on one hand, survival rate and entrapment rate are cooperatively increased; on the other hand, the plant extracts have a further effect on skin care. The external probiotics microcapsule preparation method includes the steps: (1) respectively inoculating freeze-dried powder of bifidobacteria, lactobacillus acidophilus and lactobacillus rhamnosus into a triangular flask for activating treatment, enlarging fermentation cultivation and mixing fermentation liquor of the bifidobacteria, the lactobacillus acidophilus and the lactobacillus rhamnosus according to the volume ratio 3:3:4 to obtain bacterial suspension; (2) preparing nostoc commune extracts; (3) adding Tween-80 for emulsion into the bacterial suspension, the nostoc commune extracts and sodium alginate solution, curing the bacterial suspension, the nostoc commune extracts and the sodium alginate solution, filtering the bacterial suspension, the nostoc commune extracts and the sodium alginate solution by gauze after a wet capsule is formed, and flushing the wet capsule for three times by normal saline.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method of probiotic microcapsules for external use. Background technique [0002] In recent years, many bacterial fermentation products have been applied to cosmetics and external skin applications, such as bifid yeast extract, sake yeast extract, etc. Probiotic cosmetics have revolutionized the entire industry. Its appearance makes the concept of natural, healthy and organic cosmetics more popular. Probiotic cosmetics can be roughly divided into three categories: one is probiotic yogurt cosmetics, such as facial masks made with probiotic yogurt. The second is the active extract of probiotic fermentation broth, and the third is to directly add sufficient probiotics and prebiotics to cosmetics. Each of the three types of products has its own characteristics. First of all, probiotic yogurt is a good beauty product. It has more nutrients (a variety ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/99A61K8/9789A61K8/11A61Q19/00A61K36/02A61K9/50A61K35/747A61P17/00A61P37/08A61K35/745
Inventor 王建平王耿李华敏王霆赵国强
Owner 威海美合生物科技有限公司
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