Belonging to the field of food additives, the invention discloses a stabilizer, a preparation method and application thereof, and a tofu pudding and a preparation method thereof. The stabilizer comprises the following components by weight percentage: 52-80% of agar, 10-22% of polyglycerol fatty acid ester, 6-12% of gellan gum, 3-10% of carrageenan, and 1-10% of potassium chloride. The stabilizer provided by the invention not only solves the quality problems of high gelling temperature, lack of tender and smooth taste, protein denaturation after high temperature sterilization, product water exudation and the like in tofu pudding, but also greatly reduces the cost, prolongs the normal temperature shelf-life of tofu pudding, and at the same time meets the requirements of large-scale industrial production. The tofu pudding produced by the method provided by the invention can maintain a uniform and stable state at room temperature for 6 months, the jel shape is good, the taste is tender andsmooth, and no flocculation, water exudation or spoilage phenomenon arises.