Probiotic microcapsule and preparation method thereof

A technology of probiotics and microcapsules, which is applied in the field of probiotics, can solve the problems of restricting the industrial production, storage and transportation of probiotic products, and fail to solve the problems of high viable bacteria rate of probiotics, so as to prolong the storage period at room temperature and prepare The method is simple, efficient and well tolerated

Inactive Publication Date: 2020-04-21
JIANGSU ALAND NOURISHMENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the prior art of using microcapsules to protect probiotics at present, all of them fail to solve the requirement that probiotics can still maintain a high rate of viable bacteria after passing through the gastrointestinal barrier, and all need to be frozen or refrigerated for storage, which is harmful to microencapsulated probiotics The conditions for the production, storage and application of probiotics put forward higher requirements, which greatly limits the practical application of industrial production, storage, transportation and sales of probiotic products

Method used

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  • Probiotic microcapsule and preparation method thereof

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preparation example Construction

[0014] A method for preparing probiotic microcapsules, comprising the following steps: Step 1: preparing a probiotic suspension; inoculating seeds stored in glycerol tubes of probiotics stored at -20°C at 121±1°C for 15 to 20 minutes of sterilization In the cooled MRS liquid medium, under the condition of 36±1°C, the probiotics were cultured statically for 24-36 hours for initial activation; Put it into the MRS liquid medium for activation, and cultivate it for 18-24 hours at 36±1°C to obtain the probiotic culture; pour off the supernatant after centrifugation, and wash the obtained bacterial precipitate twice with 0.85% normal saline and re- The bacterial cell precipitation was obtained after separation, and the bacterial cell concentration was adjusted to 5×10 depending on the bacterial cell precipitation. 10 ~2×10 11 CFU / mL -1 ; Step 2: Prepare water-in-oil emulsion; add bacterial cell precipitation into the oil phase matrix, and make it form water-in-oil emulsion by mean...

Embodiment 1

[0017] Prepare bifidobacterium microcapsules according to the following steps:

[0018] (1) Preparation of Bifidobacterium suspension: Inoculate the seeds of Bifidobacterium glycerol tubes stored at -20°C into the MRS liquid medium after cooling at 121±1°C for 15-20 minutes. Under the condition of ±1°C, the probiotics were cultured statically for 24-36 hours, and then activated for the first time; the probiotic culture solution for the initial activation was added to the MRS liquid medium at a mass concentration of 5% for activation. , cultivated for 18-24 hours to obtain a probiotic culture; centrifuged at 5000r / min and poured out the supernatant, washed the obtained bacterial precipitate twice with 0.85% normal saline and re-separated to obtain the bacterial precipitate, and the bacterial concentration was adjusted to 2×10 11 CFU / mL -1 .

[0019] (2) Preparation of water-in-oil emulsion: Prepare an oil phase matrix, weigh 45g of palm oil and add 5g of soybean lecithin, mi...

Embodiment 2

[0024] Prepare lactobacillus microcapsules according to the following steps:

[0025] (1) Preparation of Lactobacillus suspension: Inoculate the seeds of Lactobacillus glycerol tubes stored at -20°C into the MRS liquid medium after cooling at 121±1°C for 15-20 minutes, at 36±1 Under the condition of ℃, the probiotics were cultured statically for 24-36 hours, and the initial activation was carried out; according to the proportion of the mass concentration of 5%, the probiotic culture solution for the initial activation was inserted into the MRS liquid medium for activation. After 18-24 hours, the probiotic culture was obtained; after centrifugation at 5000r / min, the supernatant was poured out, and the obtained bacterial cell precipitate was washed twice with 0.85% normal saline and re-separated to obtain the bacterial cell precipitate, and the bacterial cell concentration was adjusted to 5× 10 10 CFU / mL -1 .

[0026] (2) Preparation of water-in-oil emulsion: Prepare an oil p...

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Abstract

The invention discloses a probiotic microcapsule and a preparation method thereof, and relates to the technical field of probiotics. The probiotic microcapsule comprises an oil-phase matrix directly contacting probiotics, a continuous water-soluble wall material wrapping the oil-phase matrix, and powder particles agglomerated on the outermost layer. The preparation method comprises the following steps: mixing a probiotic liquid / probiotic powder and a fat-soluble substance to prepare a W / O emulsion; adding the emulsion prepared in the previous step into a prepared aqueous phase solution to prepare a W / O / W double-layer emulsion; and carrying out centrifugal spray agglomeration and built-in fluidized bed drying to obtain the probiotic microcapsules. According to the invention, the microcapsules have multi-layer protection on the periphery of the probiotics, wherein the fat-soluble substances protect, then the water-soluble wall material protects the grease, and finally the peripheral agglomerated particles have isolation effect; and the probiotic microcapsules prepared by the invention can be used for functional foods or dietary nutrition supplements.

Description

technical field [0001] The invention relates to the field of probiotics, in particular to a probiotic microcapsule and a preparation method thereof. Background technique [0002] Probiotics are live microorganisms that, when ingested in sufficient quantities, have a beneficial effect on the health of the host. The probiotics that have been discovered and used mainly fall into the following three categories: ① Lactobacilli, ② Bifidobacteria, and ③ other genera. The above three major types of probiotics are all derived from human or animal bodies, among which Lactobacillus and Bifidobacterium are the most commonly used intestinal bacteria that can produce lactic acid, such as Lactobacillus acidophilus, Lactobacillus rhodus, murine Lactobacillus lysus, Bifidobacterium longum, Bifidobacterium breve, etc. [0003] The function and mechanism of action of probiotics. The physiological functions of probiotics maintain the balance of intestinal flora and improve intestinal function...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/135A23P10/30A23P10/35B01J13/02B01J13/04
CPCA23L33/135A23P10/30A23P10/35B01J13/02B01J13/043A23V2002/00A23V2200/224A23V2200/222A23V2200/3204A23V2250/18A23V2250/5114A23V2250/5118A23V2250/54252A23V2250/628A23V2250/636A23V2300/41A23V2300/10A23V2300/48A23V2250/192A23V2250/5028A23V2250/5108
Inventor 陈中科张国栋夏旭东张兴洪靖束磊
Owner JIANGSU ALAND NOURISHMENT
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