Preparation method and application of daily chemical microencapsulated probiotic
A technology of probiotics and microcapsules, applied in cosmetic preparations, preparations for skin care, cosmetics, etc., can solve the problems of the decrease of the number of probiotics, the short survival period of probiotics, and the small number of probiotics, and improve the stability at room temperature. , Extend the shelf life at room temperature, and improve the effect of anti-oxidation
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Embodiment 1
[0019] (1) Inoculate 40g each of the probiotics Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus rhamnosus into 2000mL Erlenmeyer flasks, culture at 37°C for 48h, expand the fermentation culture, when the concentration of viable bacteria in the fermentation liquid reaches 1010cfu / g, and microscopic examination showed no miscellaneous bacteria, and the morphology of the probiotics was normal, so the fermentation was terminated; the three fermentation broths were centrifuged separately, and after washing twice with 50 mL of sterile water, wet cells of the three kinds of probiotics were obtained; Mix 9g of Lactobacillus acidiformis, 7g of Lactobacillus plantarum and 4g of wet cells of Lactobacillus rhamnosus, mix with 40g of freeze-drying protectant, emulsify for 15 minutes, pre-freeze at -40°C for 12h, freeze-dry at -80°C to obtain live bacteria powder , the three bacterial powders all reached 1011cfu / g;
[0020] (2) Mix 10 g of freeze-dried bacterial powder...
Embodiment 2
[0025] (1) Inoculate 20g each of the probiotics Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus rhamnosus into a 3000mL Erlenmeyer flask, culture at 37°C for 54 hours, then expand the fermentation culture, when the concentration of viable bacteria in the fermentation broth reaches 1010cfu / g, and microscopic examination showed no miscellaneous bacteria, and the morphology of the probiotics was normal, so the fermentation was terminated; the three fermentation broths were centrifuged separately, and after washing twice with 50 mL of sterile water, wet cells of the three kinds of probiotics were obtained; 10.8g of Lactobacillus acidum, 8.4g of Lactobacillus plantarum and 4.8g of wet Lactobacillus rhamnosus were mixed with 36g of lyoprotectant, emulsified for 20 minutes, pre-frozen at -40°C for 8 hours, and freeze-dried at -80°C to obtain live bacteria powder;
[0026] (2) Mix 15g of freeze-dried bacteria powder with 75g of gelatin, add 0.5g of TG enzyme, emu...
Embodiment 3
[0031] (1) Inoculate 10g each of the probiotics Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus rhamnosus into a 3000mL Erlenmeyer flask, culture at 37°C for 60h, then expand the fermentation culture, when the concentration of viable bacteria in the fermentation broth reaches 1010cfu / g, and microscopic examination showed no miscellaneous bacteria, and the morphology of the probiotics was normal, so the fermentation was terminated; the three fermentation broths were centrifuged separately, and after washing twice with 50 mL of sterile water, wet cells of the three kinds of probiotics were obtained; Mix 9.9g of Lactobacillus acidiformis, 7.7g of Lactobacillus plantarum and 4.4g of wet cells of Lactobacillus rhamnosus, mix with 22g of freeze-drying protectant, emulsify for 30 minutes, pre-freeze at -55°C for 8h, and freeze-dry at -80°C to obtain live bacteria powder;
[0032] (2) Mix 8 g of freeze-dried bacteria powder with 48 g of gelatin, add 0.025 g of TG...
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