Preparation method and application of daily chemical microencapsulated probiotic

A technology of probiotics and microcapsules, which is applied in the direction of cosmetic preparations, skin care preparations, cosmetics, etc., can solve the problems of short survival period of probiotics, decrease in the number of probiotics, and small number of probiotics, and extend the shelf life at room temperature , Improve the effect of anti-oxidation and stability at room temperature

Active Publication Date: 2013-06-19
BIOGROWING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although probiotics have many important physiological functions on the skin, in order to obtain the desired therapeutic and health effects, probiotics must reach a sufficient number, but probiotics are relatively fragile and easily affected by surrounding environmental factors, resulting in the development of The probiotics in the product have a short survival period, and when they enter the body, they are easily affected by skin secretions, causing the number of probiotics to drop rapidly again, resulting in a very small number of probiotics that finally enter the skin and cannot effectively exert their beneficial effects effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Inoculate 40g each of the probiotics Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus rhamnosus into 2000mL Erlenmeyer flasks, culture at 37°C for 48h, expand the fermentation culture, when the concentration of viable bacteria in the fermentation liquid reaches 1010cfu / g, and microscopic examination showed no miscellaneous bacteria, and the morphology of the probiotics was normal, so the fermentation was terminated; the three fermentation broths were centrifuged separately, and after washing twice with 50 mL of sterile water, wet cells of the three kinds of probiotics were obtained; Mix 9g of Lactobacillus acidiformis, 7g of Lactobacillus plantarum and 4g of wet cells of Lactobacillus rhamnosus, mix with 40g of freeze-drying protectant, emulsify for 15 minutes, pre-freeze at -40°C for 12h, freeze-dry at -80°C to obtain live bacteria powder , the three bacterial powders all reached 1011cfu / g;

[0020] (2) Mix 10 g of freeze-dried bacterial powder...

Embodiment 2

[0025] (1) Inoculate 20g each of the probiotics Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus rhamnosus into a 3000mL Erlenmeyer flask, culture at 37°C for 54 hours, then expand the fermentation culture, when the concentration of viable bacteria in the fermentation liquid reaches 1010cfu / g, and microscopic examination showed no miscellaneous bacteria, and the morphology of the probiotics was normal, so the fermentation was terminated; the three fermentation broths were centrifuged separately, and after washing twice with 50 mL of sterile water, wet cells of the three kinds of probiotics were obtained; 10.8g of Lactobacillus acidum, 8.4g of Lactobacillus plantarum and 4.8g of wet Lactobacillus rhamnosus were mixed with 36g of lyoprotectant, emulsified for 20 minutes, pre-frozen at -40°C for 8 hours, and freeze-dried at -80°C to obtain live bacteria powder;

[0026] (2) Mix 15g of freeze-dried bacteria powder with 75g of gelatin, add 0.5g of TG enzyme, em...

Embodiment 3

[0031] (1) Inoculate 10g each of the probiotics Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus rhamnosus into a 3000mL Erlenmeyer flask, culture at 37°C for 60h, then expand the fermentation culture, when the concentration of live bacteria in the fermentation liquid reaches 1010cfu / g, and microscopic examination showed no miscellaneous bacteria, and the morphology of the probiotics was normal, so the fermentation was terminated; the three fermentation broths were centrifuged separately, and after washing twice with 50 mL of sterile water, wet cells of the three kinds of probiotics were obtained; Mix 9.9g of Lactobacillus acidiformis, 7.7g of Lactobacillus plantarum and 4.4g of wet cells of Lactobacillus rhamnosus, mix with 22g of freeze-drying protectant, emulsify for 30 minutes, pre-freeze at -55°C for 8h, and freeze-dry at -80°C to obtain live bacteria powder;

[0032] (2) Mix 8 g of freeze-dried bacteria powder with 48 g of gelatin, add 0.025 g of TG ...

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PUM

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Abstract

The invention provides a method for preparing daily chemical microencapsulated probiotic. The method comprises the steps that: first, three probiotics which are lactobacillus acidophilus, lactobacillus plantarum and lactobacillus rhamnosus are fermented and centrifuged; such that wet cells are obtained; emulsifying, pre-freezing and freeze-drying are carried out; First embedding is carried out upon the obtained probiotic powder by using a crosslinked product of gelatin and TG enzyme; and second embedding is carried out by using a mixture of soluble starch, maltose and maltodextrin. With the method, the prepared microencapsulated probiotic has an embedding rate reaching 87%. The microencapsulated probiotic can be used in daily chemicals used for inhibiting acnes, relieving allergy symptoms, and lightening spots.

Description

Technical field: [0001] This product relates to a preparation method of probiotics, in particular to a preparation method of microencapsulated probiotics for daily use. Background technique: [0002] In recent years, many functional foods have been gradually applied to cosmetics and care products, and probiotics are no exception. It can be said that probiotic cosmetics have brought about changes in the entire industry. Its appearance makes the concept of natural, healthy and organic cosmetics more popular. [0003] Probiotic cosmetics can be roughly divided into three categories: one is probiotic yogurt cosmetics, such as facial masks made with probiotic yogurt. The second is the active extract of probiotic fermentation broth, and the third is to directly add sufficient probiotics and prebiotics to cosmetics. Each of the three types of products has its own characteristics. First of all, probiotic yogurt is a good beauty product. It has more nutrients (a variety of amino ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/04C12N11/10A61K8/99A61Q19/00
Inventor 彭建
Owner BIOGROWING CO LTD
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