Formaldehyde-free animal specimen fixed liquid and preparation method thereof
A technique for fixing specimens and animals, which is applied to the preservation, application, and animal husbandry of human or animal bodies. It can solve problems such as poor penetration and poor organ fixation effects, and achieve good teaching effects and good preservation effects.
Inactive Publication Date: 2015-09-16
QINGDAO AGRI UNIV
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- Abstract
- Description
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- Application Information
AI Technical Summary
Problems solved by technology
The main component of this fixative is ethanol, which has poor penetration and poor fixation effect on large organs
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment 1
[0021] Add water to 5 g of chitosan, 10 ml of dodecyltrimethylammonium chloride, 100 ml of 95% ethanol, 20 ml of acetic acid, and 10 ml of diethylene glycol to 1000 ml to obtain a formaldehyde-free specimen fixative.
Embodiment 2
[0023] Add 5 g of chitosan, 50 ml of dodecyltrimethylammonium chloride, 100 ml of 95% ethanol, 20 ml of acetic acid, 10 ml of diethylene glycol, and 10 ml of glycerin to 1000 ml to obtain a formaldehyde-free specimen fixative.
Embodiment 3
[0025] Add 5 g of chitosan, 25 ml of didecyldimethylammonium bromide, 100 ml of 95% ethanol, 20 ml of trichloroacetic acid (5%), and 10 ml of glycerin to 1000 ml to obtain a formaldehyde-free specimen fixative.
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The invention discloses a formaldehyde-free animal specimen fixed liquid and a preparation method thereof, the formaldehyde-free animal specimen fixed liquid includes the following components: a biocide mildewcide, a protein denaturant, a polyol moisturizing agent and balance of water, and if the biocide mildewcide is in solid state, the mass concentration of the biocide mildewcide is 5-20 g/L, if the biocide mildewcide is liquid, the volume concentration of the biocide mildewcide is 1-2%; the volume concentration of the protein denaturant is 5%-40%; and the volume concentration of the polyol moisturizing agent is 2% to 10%. The formaldehyde-free animal specimen fixed liquid does not contain formaldehyde, and is non-volatile, and harmless to the human body; a fresh animal organ is washed with normal saline, and stored in the fixed liquid, a year later, the organ is not corrupted, free of mildew spot, and free of obvious color and shape change, the fixed liquid color has no obvious change, and the storage effect is good.
Description
technical field [0001] The invention relates to an animal specimen fixative, in particular to a formaldehyde-free animal specimen fixative and a preparation method thereof. Background technique [0002] Animal tissues are affected by intracellular enzymes (lysosomal enzymes) after animal death, causing various tissues and organs to soften or liquefy. This phenomenon is called autolysis. The autolysis of the midbrain, spinal cord, pancreas, and adrenal gland in various organs occurred relatively quickly, and even the cell structure completely disappeared; Other organs will also dissolve due to the action of digestive juices. In addition, the bacteria in the animal body and the environment will multiply after the death of the animal and cause the decomposition of protein in the tissue. Therefore, the preservation solution of the specimen should have the functions of sterilization, anti-mildew and protein denaturation. [0003] At present, formaldehyde solution (formalin) is...
Claims
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IPC IPC(8): A01N1/00
Inventor 李方正刘春英
Owner QINGDAO AGRI UNIV
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