The invention discloses a culture medium for pleurotus eryngii strain cultivation. The culture medium is prepared from, by weight, 20-60 parts of potatoes, 10-30 parts of bran, 2-8 parts of glucose, 1-5 parts of agar powder, 2-6 parts of tryptone, 3-9 parts of highland barley extract, 2-6 parts of yeast extract, 2-6 parts of sodium chloride, 1-3 parts of magnesium sulfate, 2-4 parts of monopotassium phosphate, 2-4 parts of water, 3-9 parts of a solid medium raw material and 2-6 parts of a nutritional agent. According to the culture medium for pleurotus eryngii strain cultivation, the potatoes, the bran and the highland barley extract are taken as raw material sources of flour and crude protein, nutrient elements and carbohydrate can be fully supplemented by adding glucose, sodium chloride, magnesium sulfate, monopotassium phosphate, the water, the solid medium raw material and the nutritional agent, therefore, the culture medium can fully meet nutrients and microelements needed by production of pleurotus eryngii strains, and the production efficiency is effectively improved.