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Stirring reaction tank used for amplified culturing of mineral-leaching microorganism

A technology of leaching microorganisms and enlarging culture, applied in the field of stirring reaction tank, can solve the problems of insufficient sealing, easy to infect miscellaneous bacteria, uneven mass transfer and heat transfer, etc. heat transfer effect

Inactive Publication Date: 2014-02-26
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the technical problems of uneven mass and heat transfer, insufficient sealing and easy infection of miscellaneous bacteria in shake flasks and beakers for microbial culture, the present invention provides a simple structure for the enlarged culture and bioleaching of ore leaching microorganisms. Stirred reaction tank for amplified culture of ore leaching microorganisms with low energy consumption and soft stirring environment

Method used

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  • Stirring reaction tank used for amplified culturing of mineral-leaching microorganism
  • Stirring reaction tank used for amplified culturing of mineral-leaching microorganism
  • Stirring reaction tank used for amplified culturing of mineral-leaching microorganism

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Experimental program
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Effect test

Embodiment 1

[0030] A nickel ore in Mojiang, Yunnan was used in the test. From the XRD diffraction pattern, the main minerals of the sample are quartz, pyrite and mica. The phase analysis results of nickel in the sample are 0.12% nickel oxide, 0.44% nickel sulfide, 0.040% nickel in silicate, and 0.60% TNi. The experiments compared the experimental effects of chemical leaching in shake flasks and reactors, and verified that the reactors can perform chemical leaching reactions at different temperatures.

[0031] The composition analysis of the nickel ore sample is shown in Table 1-1

[0032] Table 1-1 Sample composition analysis, %

[0033] TF FeO Fe 2 o 3 SiO 2 Al 2 o 3 CaO MgO MnO K 2 o Na 2 o Cu 13.34 1.60 17.30 56.14 6.76 0.62 0.86 0.084 1.81 0.064 0.038 Pb Zn P V 2 o 5 co Ni Cr 2 o 3 C S Ig 0.010 0.021 0.026 0.037 0.036 0.60 0.92 0.028 14.46 11.83

[0034] Select a 3L beaker and a 3L ...

Embodiment 2

[0038]The expanded culture of the bacteria was carried out in a 10L stirred reaction tank, and the rotating speed of the stirring blade was 300r / min. The temperature of the water in the tank of the reactor was kept constant at 45°C, and the air was pumped in by the air pump. Timely replenish the evaporated water in the reactor. Add 5000mL of 9K medium, add 200mL of activated moderate thermophilic leaching microorganisms, add 35g / L ferrous sulfate and 10g / L sulfur element as energy substances. The pH of the solution was adjusted to 1.5 with 1:1 sulfuric acid. The change of the pH value in the solution is detected with a pH acidity indicator every day, and the change of the number of ore-leaching bacteria in the solution is detected with a hemocytometer.

[0039] After 7 days of shaking flask culture, the highest bacterial concentration reached 8.0×10 8 individual / mL. However, due to the good phase and mass transfer effect in the stirred reaction tank, the mixing and ventila...

Embodiment 3

[0041] The leaching test of a chalcopyrite concentrate was carried out by using the above-mentioned moderate thermophilic leaching bacteria. First, the bacteria were domesticated and cultivated in a shake flask. When the highest bacterial concentration reached the maximum concentration of 7.5× 10 8 Individual / mL was considered as acclimatization completed. It shows that the moderately thermophilic bacteria have gradually adapted to the growth of chalcopyrite as energy material, and the ability of leaching chalcopyrite has been gradually enhanced. The leaching rate of chalcopyrite was tested without stirring speed, and the optimum stirring speed was 350r / min. The optimal aeration intensity was tested at 300mL / min, 400mL / min, 500mL / min, and 600mL / min respectively, and it was obtained that when the aeration intensity was 500mL / min, chalcopyrite concentrate was leached by moderate thermophilic bacteria after domestication. The leaching time was 30 days, the copper ion concentrat...

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Abstract

A disclosed stirring reaction tank used for amplified culturing of mineral-leaching microorganism comprises a hollow inner cylinder provided with an opening at the top, a thermal insulation device, a gas disperser and a baffle plate; the thermal insulation device is arranged at the outside of the inner cylinder; the gas disperser is a breather pipe, one end of the breather pipe is connected with an external gas source, and the other end of the breather pipe stretches into the inner cylinder and extends to the bottom of the inner cylinder via the top opening of the inner cylinder; and the baffle plate is arranged on the inner sidewall of the inner cylinder. The technical effects of the stirring reaction tank comprise that: the thermal insulation device provided by the stirring reaction tank self helps to furthest realize uniform heat transfer; the baffle plate in the stirring reaction tank helps to eliminate vortexes, change the rotary movement of the liquid into vertical rotary movement, so that the axial-direction and radial velocity components are increased, the stirring reaction tank discharged flow possesses relatively wide flow radius, and good mixing and leaching effects are realized; and because the gas disperser in the stirring reaction tank is disposed at the bottom of the stirring reaction tank, so that oxygen and carbon dioxide are fully supplemented.

Description

technical field [0001] The invention relates to the technical field of microbial hydrometallurgy, in particular to a stirring reaction tank for enlarged cultivation of ore leaching microorganisms. Background technique [0002] Microbial metallurgy is a high-tech technology that can significantly improve the development and utilization of low-grade, complex and refractory mineral resources, and can greatly improve the development and utilization of mineral resources and the degree of resource protection. Microbes use minerals as nutrients to oxidize and decompose minerals, so that metals enter the solution and then extract metals through further separation, enrichment, and purification. It has many significant advantages such as short process, low cost, environmental friendliness and low pollution. etc. [0003] At present, the seeds of ore leaching microorganisms have been cultivated in shake flasks. However, due to the small size of shake flasks, only a small amount of str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C22B3/18C22B3/02B01J19/18
CPCY02P10/20
Inventor 王军覃文庆朱珊赵红波张雁生杨聪仁程宏伟胡明皓胡岳华姜涛邱冠周
Owner CENT SOUTH UNIV
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