Pseudomonas aeruginosa chromogenic culture medium and method for quick detection by same

Abstract

The invention relates to the technical field of pseudomonas aeruginosa detection and discloses a pseudomonas aeruginosa chromogenic culture medium and a method for quick detection by the same. The pseudomonas aeruginosa chromogenic culture medium comprises a basic medium, a chromogenic substrate and an antibacterial agent. The chromogenic substrate comprises 2,3,5-phenyl tetrazolium chloride, 4-methyl umbelliferone-D-glucuronide, and the antibacterial agent comprises nalidixic acid, cycloserine and bile salt. 2,3,5-phenyl tetrazolium chloride acts on a pseudomonas aeruginosa metabolite to showa red color; 4-methyl umbelliferone-D-glucuronide acts on pseudomonas aeruginosa to show fluorescence under a 366nm ultraviolet lamp; by joint action of 2,3,5-phenyl tetrazolium chloride and the antibacterial agent, growth of common disturbance bacteria can be inhibited, and false positive results are avoided. By adoption of the pseudomonas aeruginosa quick detection method, detection and count of pseudomonas aeruginosa can be finished in 24h, and time saving, labor saving, stability, reliability and high sensitivity are realized.

Description

technical field [0001] The invention relates to the technical field of Pseudomonas aeruginosa detection, more specifically, it relates to a chromogenic culture medium for Pseudomonas aeruginosa and a method for rapid detection thereof. Background technique [0002] Pseudomonas aeruginosa (Pseudomonas aeruginosa) belongs to the genus Pseudomonas, is widely distributed in nature, can survive in water for a long time, and is considered to be a water-borne conditional pathogen; Pseudomonas aeruginosa can cause Diarrhea and other diseases in normal people, patients with metabolic diseases, blood diseases and malignant tumors, as well as postoperative patients or patients after certain treatments are susceptible to infection with this bacteria; this bacteria often causes postoperative wound infections, and can also cause bedsores, abscesses, suppuration Infection lesions caused by this bacterium can lead to hematogenous dissemination, resulting in bacteremia and sepsis. Infection ...

AI Technical Summary

Problems solved by technology

[0006] There may be Pseudomonas aeruginosa or other interfering interfering bacteria in the water sample. Therefore, after the above method is used to filter and culture the water sample for 24h to 48h, interfering colonies will usually appear, that is, fluorescent (non-blue / green) colonies or red colonies will appear. For brown colonies, it takes at least 1-5 days for further biochemical experiments to determine whether these colonies are Pseudomonas aeruginosa. The comprehensive detection time takes about 5-7 days, which is very time-consuming and laborious; and in After culture, the colonies of Pseudomonas aeruginosa and other interfering bacteria need to be judged by the naked eye of a professional experimenter on the color and shape of the colony. Colony fusion may occur if the colony is overgrown, which is a test of whether the experimenter has rich work experience. When Pseudomonas aeruginosa mutates, the shape and color of the colony change, it is difficult to judge from the color and shape of the colony, and it is easy to cause missed judgments and misjudgments

Images