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Method for screening out drought tolerant paddy rice through dynamic ion flow based on non-invasive micro-test technology (NMT)

A dynamic ion flow and detection technology, applied in the field of drought-resistant plant screening, can solve the problems of waste of rare materials and inability to realize dynamic detection of living biological materials, etc., and achieve the effects of short time-consuming, simple and reliable evaluation method, and obvious comparison difference

Active Publication Date: 2014-05-07
BEIJING RES CENT OF INTELLIGENT EQUIP FOR AGRI
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Problems solved by technology

The above methods cannot realize the dynamic detection of living biological materials and cause waste of rare materials

Method used

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  • Method for screening out drought tolerant paddy rice through dynamic ion flow based on non-invasive micro-test technology (NMT)
  • Method for screening out drought tolerant paddy rice through dynamic ion flow based on non-invasive micro-test technology (NMT)
  • Method for screening out drought tolerant paddy rice through dynamic ion flow based on non-invasive micro-test technology (NMT)

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Embodiment

[0026] 1. Experimental method

[0027] Experimental materials and culture conditions: rice varieties Nipponbare and Brazilian upland rice, the seeds were washed with sodium hypochlorite sterilized distilled water, soaked in appropriate water for 2 hours, placed on a petri dish with wet filter paper on the ground, and germinated in a light incubator at 25°C. After germination, add Hogland nutrient solution culture, PEG6000 treatment was added when it grew to the stage of 1 leaf and 1 heart, and the ion current before and after treatment was tested.

[0028] 2. Experimental instruments and consumables:

[0029]A non-destructive micro-measurement system (BIO-001B, Younger USA Sci.&Tech.Corp., USA) for ion current detection, system software imFlux, and a glass microelectrode with a tip diameter of 2-4 μm were used as microelectrodes.

[0030] 3. Experimental reagents and solutions:

[0031] (1) Liquid ion exchangers include:

[0032] K + : Cat. No. Potassium ionophore I-cockta...

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Abstract

The invention provides a method for screening out drought tolerant paddy rice through a dynamic ion flow based on NMT. According to the method, a micro-dynamic ion flow detection technology is utilized to detect the K<+> absorption capability of paddy rice roots, and the K<+> absorption change of seedlings before and after the processing by a PEG6000 solution is detected to screen out the drought tolerant paddy rice. The method allows lossless, rapid and in vivo detection of the drought tolerance of paddy rice to be realized, the detection time to be about 10-15min, the consumed time to be short, the detection accuracy to be high, the contrast difference between static ion flows of drought tolerant seedlings and non-drought tolerant drought seedlings to be obvious, and the evaluation method to be simple and reliable, so a direct and rapid screening tool is provided for the paddy rice drought tolerance breeding and the paddy rice field production.

Description

technical field [0001] The invention relates to a screening method for drought-resistant plants, in particular to a method for screening drought-resistant rice based on dynamic ion flow of non-damage micro-testing technology. Background technique [0002] Global water shortages and the continuous decline of groundwater levels lead to frequent occurrence of global drought disasters. But our country is a big agricultural country, the drought seriously endangers our country's agricultural production and food security, and restricts our country's agricultural development. According to the statistical data released by the National Flood Control and Drought Relief Headquarters, in 2009, 2010, and 2011, it suffered serious losses due to drought for three consecutive years. Drought has caused a large loss of national income and seriously restricted the development of my country's economy and society. Therefore, finding or accelerating the cultivation of new varieties of drought-re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/416A01G7/06
Inventor 陈立平乔晓军王晓冬罗斌朱大洲高权
Owner BEIJING RES CENT OF INTELLIGENT EQUIP FOR AGRI
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