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Quick detection method of dexamethasone

A detection method, the technology of dexamethasone, is applied in the field of analytical chemical detection, which can solve the problems of high detection requirements, insufficient fastness and flexibility, and high cost, and achieve the effect of obvious phenomenon, high accuracy rate, and easy operation

Inactive Publication Date: 2017-03-22
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the shortcomings of high detection requirements, high cost, and insufficient fastness and flexibility in the detection of dexamethasone in the prior art. The present invention provides a rapid detection method for dexamethasone, which has the advantages of low analysis cost and no need for comparison It has the advantages of strong specificity, high sensitivity, and high accuracy of test results, and is suitable for on-site rapid detection of whether dexamethasone is added to Chinese patent medicines, health foods, and foods.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Detection of tablets

[0028] (1) Reagent preparation: the extract is ethyl acetate; 1mol / L hydrochloric acid solution: take 9ml of hydrochloric acid, add an appropriate amount of water to make 100ml, shake well; 1mol / L sodium hydroxide solution: take 4g of sodium hydroxide, add 100ml of water, shake Shake to dissolve; Triphenyltetrazolium chloride test solution: take 0.5g of triphenyltetrazolium chloride, add absolute ethanol to dissolve it to 100ml,

[0029] (2) Sample to be tested: Antibacterial and anti-inflammatory tablets (produced by Jiangxi Boshida Pharmaceutical Co., Ltd., batch number: 20151102, specification 0.25g, determined by LC-MS / MS method that this product does not contain dexamethasone),

[0030] (3) Detection method, the steps are: take 5 tablets, remove the colored coating, grind finely, take 0.5g, put in a centrifuge tube with a stopper, add 5ml of 1mol / L hydrochloric acid solution, shake for 1min, then add 5ml of ethyl acetate , Shake vigorous...

Embodiment 2

[0032] Example 2: Detection of simulated positive tablet samples

[0033] (1) Reagent preparation: the extract is ethyl acetate; 1mol / L hydrochloric acid solution: take 9ml of hydrochloric acid, add an appropriate amount of water to make 100ml, shake well; 1mol / L sodium hydroxide solution: take 4g of sodium hydroxide, add 100ml of water, shake Shake to dissolve; Triphenyltetrazolium chloride test solution: Take 0.5g of triphenyltetrazolium chloride, add absolute ethanol to dissolve it to 100ml,

[0034] (2) Sample to be tested: Anti-bacterial anti-inflammatory tablets (produced by Jiangxi Boshida Pharmaceutical Co., Ltd., batch number: 20151102, specification 0.25g), take 5 tablets, remove the coloring coating, and add dexamethasone 10mg,

[0035] (3) The detection method, the steps are: take 5 tablets, remove the colored coating, add 10 mg of dexamethasone, grind finely, take 0.5 g, put in a centrifuge tube with a stopper, add 5 ml of 1 mol / L hydrochloric acid solution, shake for 1 ...

Embodiment 3

[0037] Example 3: Detection of syrup

[0038] (1) Sample to be tested: Strong batch of lotion (produced by Weifang Tianyang Pharmaceutical Co., Ltd., batch number: 160401, specification 180ml / bottle, determined by LC-MS / MS method that this product does not contain dexamethasone),

[0039] (2) Detection method, the steps are: take 3ml, place it in a centrifuge tube with stopper, add 5ml of 0.5mol / L hydrochloric acid solution, shake for 1min, then add 5ml of ethyl acetate, shake vigorously for 3min, centrifuge to separate, take For the upper layer solution, add an equal volume of 0.5mol / L sodium hydroxide solution, shake for 1 min, centrifuge to separate, and use the upper layer as the test solution; take 1 drop of 0.5mol / L sodium hydroxide solution and place a white porcelain drip plate On the top, add 5 drops of triphenyltetrazolium chloride test solution, mix well, add 5 drops of test solution, the color is negative (not red).

[0040] Result analysis: This product does not contain...

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PUM

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Abstract

The invention relates to the technical field of analytical chemical detection, in particular to a quick detection method of dexamethasone; comprising the steps of taking a sample to be detected adding hydrochloric acid solution, shaking, adding ethyl acetate, shaking, and centrifuging or standing to allow layering; taking supernate or upper solution, adding sodium hydroxide solution, shaking, and centrifuging or standing to allow layering; dropping sodium hydroxide solution to a white dropper plate, dropwise adding triphenyl tetrazolium chloride test solution, mixing well, dropwise adding test sample solution, and observing changes in color. The method of the invention is simple to perform and low in analytical cost; despite of the influence of related factors, the observation of solution color is more facilitated, the abstracting liquid, washing liquid, extracting liquid and detection agents used herein are highly specific, interfering substances are separated and removed by using dissolution differences of different substances three times in the abstracting, washing and extracting processes, the sensitivity of color reaction is improved, detection results accuracy is high, the color reaction is quick, phenomena are significant, and the method is suitable for quick field detection.

Description

Technical field [0001] The invention relates to the technical field of analytical chemistry detection, in particular to a rapid detection method of dexamethasone. Background technique [0002] In recent years, people have paid more and more attention to health and drug safety. Since most of the health care products and Chinese patent medicines are derived from natural substances, they have mild curative effects and high safety. They are favored by patients at home and abroad in the treatment of chronic diseases such as asthma. , But it has the shortcomings of slow onset or insignificant effect. In order to make huge profits, some criminals take advantage of people’s eagerness to achieve results, and without any pharmacological and toxicological studies, they randomly and illegally add chemical drugs with fast-acting and definite effects. Chemical drugs with anti-tussive effect are common at present. Theophylline, dexamethasone and so on. The chemical components of health care...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 李冰高辉
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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