Method for quantitatively determining viability of barley seeds

Abstract

The invention provides a method for quantitatively determining viability of barley seeds, belongs to the technical field of evaluation of the viability of the barley seeds, and can evaluate germination potential of the barley seeds rapidly and stably and provide reference for seed purchase and production. According to the method, TTC (triphenyl tetrazolium chloride) is used for dyeing dissected barley seeds, the dyed barley seeds are crushed with a freeze-drying and ball-milling crushing combined technique, a 10% trichloroacetic acid methanol solution is adopted as extract liquor to perform extraction on the crushed barley seeds, TTF (1,3,5-triphenylformazan) is obtained, and content of TTF is analyzed quantitatively according to a TTF standard curve with absorption photometry to predict the viability of the barley seeds. With the adoption of the freeze-drying and ball-milling crushing combined technique, loss of a sample in an operation process is reduced, and an experimental result is more precise and more reliable. By separating and dissecting the barley seeds, interference of useless substances in follow-up experiments is reduced, reaction volume is reduced, and experiment costand workload are saved.

Description

technical field [0001] The invention belongs to the technical field of barley seed vigor evaluation, and in particular relates to a method of dyeing barley seeds with tetrazolium (TTC), further extracting with an organic solvent to obtain triphenylformazan (TTF), and adopting absorptiometry, according to triphenyl An evaluation method for predicting barley seed vigor by quantitative analysis of formazan standard curve. Background technique [0002] Seed vigor is an important evaluation index of seed quality. It is closely related to the germination rate of seeds. The rapid and accurate measurement of seed vigor can truly reflect the germination ability of seeds, especially suitable for the evaluation of seeds that cannot germinate normally during the dormant period. Currently commonly used seed vigor evaluation methods include tetrazolium (2,3,5-triphenyltetrazolium chloride, TTC) staining method, bromothymol blue (BTB) method and conductivity measurement method, etc., where...

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Problems solved by technology

[0004] However, the liquid nitrogen grinding method was used to crush the seeds in the above experiment process, which is cumbersome and laborious. At the same time, the transfer of the seeds will cause the seeds to remain on the mortar and pestle, and the liquid nitrogen treatment will mix indefinite amounts of seeds in the sample. Condensed water will cause unstable errors in the experiment and affect the accuracy of seed vigor evaluation

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