Method for quantitatively detecting molds and saccharomycetes in eye masks

Abstract

The invention belongs to the field of microbial detection and particularly discloses a method for quantitatively detecting molds and saccharomycetes in eye masks; the method comprises the steps of (1) weighing eye mask, and adding diluent of suitable volume to obtain a test liquid; (2) adding a mold liquid medium of suitable volume into the test liquid, and performing enrichment culture at 28+ / - DEG C for 48-72 h to obtain homogenous liquid; (3) collecting a suitable amount of the homogenous liquid, applying respectively to rose Bengal agar plate and triphenyl tetrazolium chloride-Sabouraud agar plate, standing, and culturing at 28+ / -1 DEG C for 3-5 days; (4) judging results, to be specific, if no colonies grow on the plates or if there are colonies grown on the plates while no suspected colonies are present, determining zero match; if it is confirmed that saccharomycetes or molds grown on any one of the plates, determining that a target bacterium is detected on the sample. The method has the advantages of high specificity, low detection limit, good reproducibility, good operational convenience and the like, and meets the requirement of manufacturers for product quality control.

Description

technical field [0001] The invention belongs to the field of microorganism detection, and in particular relates to a qualitative detection method for mold and yeast in eye patches. Background technique [0002] Health-care eye patch refers to the absorption of active ingredients such as extracts of Chinese herbal medicines with auxiliary materials (such as cotton, silk, brocade, non-woven fabrics, etc.), and sticking them on or around the eyes, so that the active ingredients can be absorbed locally to improve skin health. , A product that relieves discomfort symptoms such as eye fatigue. Mold and yeast widely exist in nature, and the main growth environment of yeast is a humid or liquid environment. The liquid environment of eye patch preparations provides favorable conditions for the reproduction and growth of mold and yeast. Mold and yeast belong to the fungus phylum, and many fungi can produce toxins. The use of polluted and deteriorated health care eye patches will caus...

AI Technical Summary

Problems solved by technology

It is required to control the production process in accordance with the GMP standard of pharmaceuticals. The production process and its environment are extremely strict on the control of microorganisms, so that the product can finally meet the requirements of the sterility index. However, health care products are non-sterile products, and the raw materials, auxiliary materials, production environment and their products cannot. To meet the requirements of sterility index, from the analysis of actual product production and control, eye patch health care products are not suitable for sterility testing

The medium used in the sterility test method is tryptone soy liquid medium, which cannot rule out the interference caused by bacteria, which may easily cause false positives

In addition, the sterility inspection method has an inspection cycle of 14 days, which is a long cycle, and the product has a long cycle from production, inspection to release, which brings troubles to the storage, transportation and production efficiency of the enterprise.

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