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Method for determining vitality of pseudo-ginseng pollen

A technology for pollen viability and determination method, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, plant cells, etc., can solve the problems of cumbersome procedures, long time consumption, low accuracy, etc. And the effect of quality promotion and simple operation

Inactive Publication Date: 2017-01-18
文山苗乡三七科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of low accuracy, cumbersome procedures, and long time-consuming of the existing pollen viability detection methods, the purpose of the present invention is to provide a method for the determination of the pollen vigor of Panax notoginseng

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A. configure the sucrose solution whose mass percent concentration is 5%, place the sucrose solution in a closed sterilizer, control the temperature at 121 degrees Celsius, control the steam pressure at 0.1 MPa, and sterilize for 30 minutes;

[0033] B. configuration mass percent concentration is 0.5% 2,3,5-chlorinated triphenyltetrazolium solution, with sucrose solution and 2,3,5-chlorinated triphenyltetrazolium solution by mass ratio 1: The ratio of 20 was mixed evenly, and irradiated under ultraviolet light for 24 hours to obtain a dyeing reaction solution, which was put into a brown glass bottle and stored under light-proof conditions, and the storage temperature was controlled at 4 degrees Celsius;

[0034] C. Drip the dyeing reaction solution on the cover glass, pick the pollen of the Panax notoginseng plant that loosened the pollen that day, put the pollen into the staining reaction solution on the cover glass, turn the cover glass over and place it on the concave...

Embodiment 2

[0039] A. configuration mass percent concentration is 2% sucrose solution, sucrose solution is placed in airtight sterilizer, temperature is controlled at 107 degrees centigrade, steam pressure is controlled at 0.025 MPa, and sterilization time is 15 minutes;

[0040] B. configuration mass percent concentration is 0.2% 2,3,5-chlorinated triphenyltetrazolium solution, with sucrose solution and 2,3,5-chlorinated triphenyltetrazolium solution by mass ratio 1: The ratio of 10 was mixed evenly, and irradiated under ultraviolet light for 20 hours to obtain a dyeing reaction solution, which was put into a brown glass bottle and stored under light-proof conditions, and the storage temperature was controlled at 1 degree Celsius;

[0041] C. Drip the dyeing reaction solution on the cover glass, pick the pollen of the Panax notoginseng plant that loosened the pollen that day, put the pollen into the staining reaction solution on the cover glass, turn the cover glass over and place it on t...

Embodiment 3

[0045] A. configuration mass percent concentration is 10% sucrose solution, sucrose solution is placed in airtight sterilizer, temperature is controlled at 134.5 degrees centigrade, steam pressure is controlled at 0.2 MPa, and sterilization time is 40 minutes;

[0046] B. configuration mass percent concentration is 0.8% 2,3,5-triphenyltetrazolium chloride solution, with sucrose solution and 2,3,5-triphenyltetrazolium chloride solution in mass ratio 1: Mix evenly at a ratio of 30, irradiate under ultraviolet light for 30 hours to obtain a dyeing reaction solution, put it into a brown glass bottle, and store it under dark conditions. The storage temperature is controlled at 8 degrees Celsius;

[0047] C. Drip the dyeing reaction solution on the cover glass, pick the pollen of the Panax notoginseng plant that loosened the pollen that day, put the pollen into the staining reaction solution on the cover glass, turn the cover glass over and place it on the concave slide, so that The s...

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Abstract

The invention relates to a method for determining vitality of pseudo-ginseng pollen. The method comprises the following steps: using a cane sugar solution and a 2,3,5-triphenyl tetrazolium chloride solution to prepare a dyeing reaction solution; dropping the dyeing reaction solution on a cover glass, selecting the pseudo-ginseng pollen in the dyeing reaction solution on the cover glass, overturning the cover glass and placing the cover glass on a concave slide glass; placing the pollen in a thermotank for culturing; taking the pollen out and placing the pollen under a microscope for observation, selecting the visual field at random, and counting the proportion of dyed pollen grain number and the undyed pollen grain number, and wherein the pseudo-ginseng pollen with 30% or more has vitality. The method has the beneficial effects of simple operation, convenient and practical performances, high accuracy, and reliable statistics data, and has high practical application value, and has active promotion effect for breeding efficiency, output and quality of pseudo-ginseng.

Description

technical field [0001] The invention belongs to the technical field of agricultural planting, and in particular relates to a method for measuring the vigor of notoginseng pollen. Background technique [0002] Panax notoginseng is a plant of the genus Panax of Araliaceae, also known as Tianqi, Jinbuhuan, etc. In ancient times, it was also known as Zhaoshen, blood ginseng, panax notoginseng, Tiansanqi, mountain lacquer, Panax notoginseng, etc. Its roots, stems, leaves It is a rare and precious Chinese medicinal material unique to my country, and it is also one of the earliest medicinal and edible plants in China. Sanqi is warm in nature, sweet and slightly bitter, and has been recognized since ancient times as having significant effects of promoting blood circulation, removing blood stasis, It has the effect of relieving swelling and relieving pain, and has the reputation of "sacred grass in the south". [0003] Modern pharmacological research shows that Panax notoginseng conta...

Claims

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Application Information

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IPC IPC(8): C12N5/04C12Q1/02
CPCC12N5/04C12Q1/02
Inventor 陈中坚王勇魏富刚刘伟林余育启
Owner 文山苗乡三七科技有限公司
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