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Low-temperature dry preservation method for paphiopedilum pollen

A preservation method and pollen technology, which are applied in the field of plant reproduction, can solve problems such as inconsistency between the species of Paphiopedilum and the flowering period of hybrids, and achieve the effects of major social and economic benefits and strong pollen vigor.

Inactive Publication Date: 2017-12-15
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The crossbreeding of Paphiopedilum in my country has just started. Although my country has abundant Paphiopedilum resources (about 30 kinds), the flowering periods of many Paphiopedilum species and hybrids are inconsistent, and fresh pollen cannot be used for crossbreeding among them.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment 1: Helen Paphiopedilum (Paphiopedilum helenae) self-pollination of preserved pollen

[0011] (1) Collection and storage of pollen

[0012] In October, when the flowers of Helen's Paphiopedilum fully unfolded for 3 days, the medicine cap was carefully opened with a toothpick sterilized by high temperature and high pressure at 10:00 am, and then the pollen mass after flowering was pasted with a sterilized toothpick and quickly placed in a clean place. In sterile 1mL plastic centrifuge tubes, put 1 pollen mass into each tube, and immediately cover and seal the centrifuge tubes. Replace with a new toothpick every time you take 1 pollen block. Then put the centrifuge tube with pollen mass in a sterile glass bottle, seal it and bring it back to the laboratory. Then under aseptic condition, open the cover of the plastic centrifuge tube for storing pollen, cover the tube opening with sterile absorbent cotton, put it into a glass bottle with silica gel desiccant for...

Embodiment 2

[0021] Embodiment 2: Helen Paphiopedilum (Paphiopedilum helenae) preserves pollen and the hybrid pollination of Paphiopedilum helenae

[0022] (1) Collection and storage of pollen

[0023] In October, 4 days after the flowers of Helen Paphiopedilum fully unfolded, the medicine cap was carefully opened at 11 am with a high-temperature and high-pressure sterilized toothpick, and then the pollen mass after flowering was glued with a sterilized toothpick and quickly placed on the Put 1 pollen block into a clean and sterile 1mL plastic centrifuge tube, and immediately cover and seal the centrifuge tube. Replace with a new toothpick every time you take 1 pollen block. Then put the centrifuge tube with pollen mass in a sterile glass bottle, seal it and bring it back to the laboratory. Then under aseptic condition, open the cover of the plastic centrifuge tube for storing pollen, cover the tube opening with sterile absorbent cotton, put it into a glass bottle with silica gel desicca...

Embodiment 3

[0032] Embodiment 3: the hybrid pollination of Paphiopedilum wardii preserved pollen and Paphipedilum wardii

[0033] (1) Collection and storage of pollen

[0034] In December, 5 days after the flowers of Papudola chinensis fully unfolded, at 12 o'clock in the morning, use a high-temperature and high-pressure sterilized toothpick to carefully open its medicinal cap, and then use a sterilized toothpick to stick the pollen mass after flowering and put it quickly. Put 1 pollen block into a clean and sterile 1mL plastic centrifuge tube, and immediately cover and seal the centrifuge tube. Replace with a new toothpick every time you take 1 pollen block. Then put the centrifuge tube with pollen mass in a sterile glass bottle, seal it and bring it back to the laboratory. Then under aseptic condition, open the cover of the plastic centrifuge tube storing pollen, cover the tube opening with sterile absorbent cotton, put it into a glass bottle with silica gel desiccant for dehydration ...

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Abstract

The invention discloses a low-temperature dry preservation method for paphiopedilum pollen. The method comprises the following steps that within 3-5 days when a paphiopedilum flower is completely unfolded, an anther cap of the flower is opened by using a sterilized picking tool; a paphiopedilum pollen mass is picked to a clean and sterile container by using the sterilized picking tool, and the pollen mass is dehydrated under a sterile state; the dehydrated pollen mass is well sealed, placed in an environment with the temperature of 4 DEG C and preserved in a dark place. The preservation time of the paphiopedilum pollen mass preserved by using the method can reach 6-12 months, and after the pollen mass is subjected to re-warming and rewetting, by determining the pollen viability through a triphenyl tetrazolium chloride staining method, it is found that the viability of the pollen preserved by using the method is high, and the selfing or hybridization success rate is up to 90-95%. By means of the method, the barriers of cross pollination of types and plants of paphiopedilum in different blooming periods are overcome, a foundation is laid for the further development of the culture and industries of new varieties of Chinese paphiopedilum, and therefore, the method has great social benefits and economic benefits.

Description

Technical field: [0001] The invention belongs to the technical field of plant propagation, and in particular relates to a method for low-temperature drying and preserving of paphiopedilum pollen. Background technique: [0002] Paphiopedilum is also called slipper orchid, cinderella, etc. There are more than 90 species in the world, mainly distributed in tropical Asia to Pacific islands. Paphiopedilum has a very high ornamental value due to its unique flower shape, gorgeous flower color, and long-lasting ornamental flowering period. It is a very popular high-end flower in the international flower market and has a large number of lovers in the world. However, due to the destructive excavation of wild resources due to people's excessive pursuit of P. Convention" (CITES) Appendix I and trade is prohibited. [0003] Compared with the original species, the hybrid species of Papuopsis has more graceful posture, strong growth potential and stress resistance, and more colorful flow...

Claims

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Application Information

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IPC IPC(8): A01D46/00A01H1/02A01N3/00F26B5/16
CPCA01D46/00A01H1/02A01N3/00A01H1/027F26B5/16
Inventor 郑枫曾宋君吴艳妮吴坤林
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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