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Method for detecting cell viability of fresh Cordyceps sinensis

A technology for fresh Cordyceps sinensis and Cordyceps sinensis, which is applied in the field of agricultural product detection, can solve the problems of poor repeatability and stability of Cordyceps sinensis cell viability detection, inability to accurately and truly reflect Cordyceps sinensis cell viability, low sensitivity, etc., and achieves shortened dyeing time, low cost, and low sensitivity. The effect of increasing sensitivity

Active Publication Date: 2017-03-22
YICHANG SHANCHENGSHUIDU CORDYCEPS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tetrazolium method mentioned in the above technical literature is not only time-consuming, but also has poor repeatability, stability and low sensitivity when directly applied to the detection of Cordyceps sinensis cell viability; and because it takes too long, the staining temperature is higher than that of fresh Cordyceps Storage temperature, these methods cannot accurately and truly reflect issues such as the cell viability of Cordyceps sinensis

Method used

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  • Method for detecting cell viability of fresh Cordyceps sinensis
  • Method for detecting cell viability of fresh Cordyceps sinensis
  • Method for detecting cell viability of fresh Cordyceps sinensis

Examples

Experimental program
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Effect test

Embodiment 1

[0031] (1) Take fresh Cordyceps sinensis out of the storage environment, and carry out pretreatment. The method is: place it in a 5% glucose solution preheated to 37°C and immerse it in a 37°C water bath for 15 minutes;

[0032] (2) slice the fresh Cordyceps sinensis worm body part treated in step (1), and the slicing part is the 4th to 7th pair of foot sections of the worm body, cut into 1-2mm thin slices, and cut 10 pieces;

[0033] (3) Place fresh Cordyceps sinensis slices in a small beaker equipped with 0.3% TTC solution for soaking, and place them in a water bath at 37° C. for dark dyeing;

[0034] (4) When the dyeing time is 0.5 hour, 1 hour, 2 hours, 4 hours, and 8 hours, take out 2 slices of fresh Cordyceps sinensis after dyeing, blot the surface moisture, observe the color, and take pictures.

[0035] The result is as figure 2 As shown, it can be seen from the results that after five different dyeing times, after 0.5 hours of dyeing, the Cordyceps slices began to be...

Embodiment 2

[0037] (1) Take 2 fresh Cordyceps sinensis out of the storage environment, and carry out pretreatment on one, the method is: place it in 5% glucose solution preheated to 37 ℃, immerse in 37 ℃ water bath for 15 minutes; do not pretreat the other one, directly ready for use;

[0038] (2) Slice the same parts of the fresh Cordyceps sinensis treated in step (1) and the Cordyceps sinensis without pretreatment, and the sliced ​​parts are the 4-7 pairs of legs of the insect body, cut into 1-2mm thin slices, each Cut the roots into 4 slices;

[0039] (3) Place 2 slices of fresh Cordyceps sinensis in small beakers filled with TTC solution with a mass fraction of 0.3% for soaking, and place them in a water bath at 37° C. for dark dyeing;

[0040] (4) Take out 2 sections of fresh Cordyceps sinensis when the dyeing time is 2 hours, blot the surface moisture, observe the color, and take pictures.

[0041] The result is as image 3As shown, it can be seen from the results that the color ...

Embodiment 3

[0043] (1) Take fresh Cordyceps sinensis out of the storage environment, and carry out pretreatment. The method is: place it in a 5% glucose solution preheated to 37°C and immerse it in a 37°C water bath for 15 minutes;

[0044] (2) The fresh Cordyceps sinensis processed in step (1) is divided into 4 sections and sliced, which are respectively the Cordyceps sinensis section, the worm body head to the 4th pair of feet section, the 4th-7 pair of feet section and From the seventh pair of feet to the tail, take 4 pieces from each of the 4 sections, and cut each piece into 1-2 mm thin slices;

[0045] (3) The fresh Cordyceps sinensis slices of 4 different sections were respectively placed in a small beaker with a mass fraction of 0.3% TTC solution for soaking, and placed in a 37°C water bath for dark dyeing;

[0046] (4) Take out the slices of fresh Cordyceps sinensis after 2 hours of dyeing, blot the surface moisture, observe the color, and take pictures.

[0047] The result is a...

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Abstract

The invention relates to the field of agricultural product detection, and in particular relates to a method for detecting cell viability of fresh Cordyceps sinensis. The method is mainly as follows: after pretreatment, the fresh Cordyceps sinensis is sliced, slices are placed into a small beaker with a certain concentration of a 2, 3, 5-triphenyl tetrazolium chloride solution for soaking, after light-proof thermostatic waterbath dyeing for a period of time, the dyed fresh Cordyceps sinensis slices are taken out, surface water is absorbed to dry, the dying condition of the fresh Cordyceps sinensis slices is observed, and according to color depth and dyeing uniformity, the cell viability of fresh Cordyceps sinensis can be determined. The method is a method for quickly, conveniently and accurately detecting the cell viability of the fresh Cordyceps sinensis.

Description

technical field [0001] The invention relates to the field of detection of agricultural products, in particular to a fast and simple method for detecting the viability of fresh Cordyceps sinensis cells. [0002] technical background [0003] Cordyceps sinensis is a traditional precious Chinese medicinal material, and it mostly exists in the form of its dry product in the market. Because the fresh Cordyceps sinensis maintains the original ecology, the cell vitality is well maintained, and its nutrients and functional components are better preserved, especially macromolecules such as active polypeptides and polysaccharides. Therefore, the appearance of instant fresh Cordyceps sinensis has been born in recent years. The freshness of Cordyceps sinensis has a lot to do with its fresh and crisp taste and the maintenance of nutrition and functional ingredients. Therefore, how to judge the freshness of fresh Cordyceps sinensis has also become an important issue. At present, there i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N21/25
CPCG01N1/30G01N21/25
Inventor 钱正明廖娜张蓉艾中李光荣李文佳
Owner YICHANG SHANCHENGSHUIDU CORDYCEPS
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