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Method for increasing embryo activity of mallotus tetracoccus seeds after cryopreservation

A technology for cryopreservation and seed embryos, applied in the field of plant germplasm resource preservation

Active Publication Date: 2017-12-01
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For the seeds of this species after low-temperature storage, there is no effective method to germinate them, so the evaluation of embryo viability uses the TTC staining method

Method used

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  • Method for increasing embryo activity of mallotus tetracoccus seeds after cryopreservation

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Embodiment 1

[0019] 1. Materials and methods:

[0020] 1.1 Research materials:

[0021] In October 2008, mature fresh Malotustetracoccus seeds were collected in Shuitang Town, Xinping County, Yuxi City, Yunnan Province. After collection, they were transported back to the Southwest Wild Organism Germplasm Bank Seed Preservation Center of Kunming Institute of Botany.

[0022] 1.2 Research methods:

[0023] 1.2.1 Drying and refrigerating and returning to temperature after refrigerating: put the seeds collected in step 1.1 in a drying room at 15°C and 15% relative humidity to dry. Regularly use the Hygrolab C1unit relative humidity measuring instrument produced by Rotronic Ltd. combined with the Rotronic HC 2-AW probe produced by the same company to measure the relative humidity of the seeds, and put them into a closed container after the equilibrium relative humidity reaches 15%. On August 23, 2011, the airtight container with the seeds was put into -18°C to -20°C for refrigeration. After...

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Abstract

A method for increasing embryo activity of mallotus tetracoccus seeds after cryopreservation comprises the following steps of taking dried seeds stored at the low temperature of -18 DEG C to 20 DEG C out together with a closed container for storing the seeds; placing the seeds in the environment with the temperature of 15 DEG C and the relative humidity of 15% for 24 hours, and then taking out the seeds; placing the seeds in an incubator with the temperature of 45 DEG C and taking out after 6 hours of heat treatment; placing the seeds on a 1% agar medium after the temperature of the seeds return to room temperature, and then placing at a 20 DEG C culturing room for 24 hours; cutting seed coats and embryos of the treated seeds, immersing in a 1% triphenyl tetrazolium chloride (TTC) solution for 48 hours in the dark at 30 DEG C; taking out the soaked seeds and observing embryo staining conditions, wherein embryos with activity show a uniform red color. The method significantly enhances the embryo viability of the mallotus tetracoccus seeds stored at the low temperature for more than 5 years, and solves the problem of reduction of the embryo vitality of the mallotus tetracoccus seeds after cryopreservation. The treatment method is simple and easy without any danger and side effects.

Description

technical field [0001] The invention belongs to the technical field of preservation of plant germplasm resources, and in particular relates to a method for improving the viability of the seed embryos of pauliatris simulatum after preservation at low temperature (-18°C to -20°C). Background technique [0002] Mallotus tetracoccus (Roxb.) Kurz is a small tree belonging to the genus Mallotus Lour. in the family Euphorbiaceae. Produced in southern Yunnan and Tibet, it grows in valley forests at an altitude of 500-1300 meters. This kind of extract has a certain antibacterial effect, and there is no research report on the germination and storage of the seeds of Tunga simulatum. [0003] Drying normal seeds and storing them at low temperature (-18°C to -20°C) can greatly prolong their lifespan, which has reached an international consensus, and based on this theory, several crop and wild plant seed banks have been established; but The viability of the embryos of some plants will c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02A01C1/00
CPCA01C1/00A01C1/025
Inventor 胡枭剑杨娟秦少发张志峰郭永杰何华杰杜燕李涟漪杨湘云
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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