A method for improving the vigor of tung tree seed embryos after low-temperature preservation
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology for cryopreservation and seed embryos, applied in the field of plant germplasm resource preservation
Active Publication Date: 2021-03-30
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
View PDF2 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
For the seeds of this species after low-temperature storage, there is no effective method to germinate them, so the evaluation of embryo viability uses the TTC staining method
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0020] 1. Materials and methods:
[0021] 1.1 Research materials:
[0022] In July 2013, mature fresh Mallotus barbatus seeds were collected in Changlong Village, Nanping Township, Shangsi County, Fangchenggang City, Guangxi. After collection, they were transported back to the Southwest Wild Organism Germplasm Bank Seed Preservation Center of Kunming Institute of Botany.
[0023] 1.2 Research methods:
[0024] 1.2.1 Drying and refrigerating and returning to temperature after refrigerating: put the seeds collected in step 1.1 in a drying room at 15°C and 15% relative humidity to dry. Regularly use the Hygrolab C1unit relative humidity measuring instrument produced by Rotronic Ltd. combined with the Rotronic HC 2-AW probe produced by the same company to measure the relative humidity of the seeds, and put them into a closed container after the equilibrium relative humidity reaches 15%. On November 11, 2014, the airtight container containing the seeds was placed in a refrigerat...
Embodiment 2
[0036] 1. Materials and methods:
[0037] 1.1 Research materials:
[0038] On August 13, 2012, mature fresh Mallotus barbatus seeds were collected in Jiaoshi Town, Fuling District, Chongqing. After collection, they were transported back to the Southwest Wild Organism Germplasm Bank Seed Preservation Center of Kunming Institute of Botany.
[0039] 1.2 Research methods:
[0040] 1.2.1 Drying and refrigerating and returning to temperature after refrigerating: put the seeds collected in 1.1 in a drying room at 15°C and 15% relative humidity to dry. Regularly use the Hygrolab C1unit relative humidity measuring instrument produced by Rotronic Ltd. combined with the Rotronic HC 2-AW probe produced by the same company to measure the relative humidity of the seeds, and put them into a closed container after the equilibrium relative humidity reaches 15%. On August 9, 2013, the airtight container with the seeds was placed in -18°C to -20°C for refrigeration. Remove the container with...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a method for improving the activity of mallotus barbatus seed embryos preserved at a low temperature. The method comprises the following steps: taking out dried seeds preserved at a low temperature ranging from minus 18 DEG C to minus 20 DEG C together with a closed container for storing the seeds; putting the dried seeds and the closed container in an environment with the temperature of 15DEG C and the relative humidity of 15 percent for 24 hours, and taking out the seeds; putting the seeds into an incubator with the temperature of 45DEG C, carrying out heat treatment for 6 hours and then taking out; restoring the temperature of the seeds to room temperature, putting the seeds on a 1 percent agar culture medium, and putting the seeds in a culturing room with the temperature of 20DEG C for 24 hours; cutting seed coats and endosperms of the treated seeds, soaking the treated seeds into 1 percent triphenyltetrazolium chloride (TTC) solution, and putting a mixture in a dark condition with the temperature of 30DEG C for 48 hours; taking out the soaked seeds, cutting off and observing dyeing conditions of the embryos, and displaying uniform red color for the embryos with the activity. According to the method disclosed by the invention, the problem that the activity of the embryos is reduced after mallotus barbatus seeds are preserved at low temperature is solved; in addition, the treatment method is simple and feasible and is free of risk and toxic and / or side effects.
Description
technical field [0001] The invention belongs to the technical field of plant germplasm resource preservation, and in particular relates to a method for improving the vigor of paulownia seed embryos preserved at low temperature (-18°C to -20°C). Background technique [0002] Mallotus barbatus (Wall.) Muell. Arg. is a small tree species of Euphorbiaceae (Euphorbiaceae) Mallotus Lour.. Distributed in Eastern and Southern Asian countries. It is mainly produced in Yunnan, Sichuan, Guizhou, Hunan, Guangdong and Guangxi in China. Born in forest margins or shrubs at an altitude of 400-1 300 meters. [0003] The stem bark fiber can be used as raw material for papermaking; the wood is light and soft and can be used for making utensils; the seed oil can be used as industrial oil. At present, there is no research report on the germination and storage of tung tree seeds. [0004] Drying normal seeds and storing them at low temperature (-18°C to -20°C) can greatly prolong their lifesp...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00
CPCA01C1/00
Inventor 杨娟胡枭剑郭云刚杨娅娟蔡杰杜燕秦少发李涟漪杨湘云
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
Login to view more 
Login to view more