A method for improving the endosperm viability of the seeds of Alba dorsifolia after low-temperature preservation

Abstract

A method for improving activity of seed endosperm of mallotus apelta after low temperature preservation comprises: taking out dried seeds in low temperature preservation at -18 DEG C to -20 DEG C and a sealed container storing the seeds; placing same in an environment with the temperature of 15 DEG C, and relative humidity of 15% for 24 hours, and taking out the seeds; placing the seeds in an incubator at the temperature of 45 DEG C, performing heat treatment for 4-6 hours, and then taking out the seeds; after recovering to the room temperature, placing the seeds on an agar culture-medium of 1%, and placing same at a culture chamber at 20 DEG C for 24 hours; cutting the peel and endosperm of the processed seeds, soaking in a 1% triphenyltetrazolium chloride (TTC) solution, and placing at a dark condition at 30 DEG C for 48 hours; taking out the soaked seeds, cutting same to observe dyeing conditions of the endosperm, wherein the endosperm having activity shows even red. The method obviously improves the activity of endosperm of the mallotus apelta seeds from different producing areas and stored at a low temperature for different times, and solves the problem that the endosperm of the mallotus apelta seeds is reduced after low temperature preservation. Moreover, the processing method is simple and easy to achieve without any danger or toxic and side effects.

Description

technical field [0001] The invention belongs to the technical field of plant germplasm resource preservation, and in particular relates to a method for improving the endosperm vigor of the seed endosperm of the alba dorsal leaf after low temperature (-18°C to -20°C) preservation. Background technique [0002] Mallotus apelta (Lour.) Muell.Arg. is a deciduous shrub or small tree species of Euphorbiaceae (Mallotus Lour.). It often grows on the roadsides of flat hills in southern provinces and on barren and dry Guangshan or barren hills. It has strong adaptability and can be used to prevent soil loss and ecological restoration. The young leaves in spring can be mixed with other feed to feed pigs, and the leaves can be collected in winter to make compost. Stem bark is fibrous raw material, woven into sacks, used for blending or as raw material for wax paper and rayon. The roots and leaves of the white back leaf are used as medicine; the leaves have the effects of clearing away...

AI Technical Summary

Problems solved by technology

Although white-backed leaves are widely distributed in my country, the amount of resources is relatively small. Whether it is hard branch cuttings or soft branch cuttings, the reproductive survival rate is not high, only about 30%.

At present, the cultivation technology of the white-backed leaf is not mature enough, there is no report on large-scale seedling cultivation, sowing is still one of the main propagation techniques, and there is no report on the storage of the white-backed leaf seed.

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