Method for improving vitality of endosperms of mallotus nepslensis seeds preserved at low temperature

A technology for cryopreservation and seed embryos, which is applied in the field of plant germplasm resource preservation and can solve problems such as no research reports.

Active Publication Date: 2017-09-01
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Method for improving vitality of endosperms of mallotus nepslensis seeds preserved at low temperature
  • Method for improving vitality of endosperms of mallotus nepslensis seeds preserved at low temperature
  • Method for improving vitality of endosperms of mallotus nepslensis seeds preserved at low temperature

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Embodiment 1

[0019] 1. Materials and methods:

[0020] 1.1 Research materials:

[0021] In October 2014, fresh and mature seeds of Mallotus nepalensis were collected in Xintian Village, Fuxi Township, Huizhou District, Huangshan Mountain, Anhui Province. After collection, they were transported back to the Seed Collection Center of the Southwest Wildlife Germplasm Bank of Kunming Institute of Botany.

[0022] 1.2 Research methods:

[0023] 1.2.1 Drying and refrigeration after refrigeration: The seeds collected in step 1.1 are dried in a drying room at 15°C and 15% relative humidity. The Hygrolab C1unit relative humidity meter produced by Rotronic Ltd. is used in conjunction with the Rotronic HC 2-AW probe produced by the same company to measure the relative humidity of the seeds on a regular basis. After the equilibrium relative humidity reaches 15%, it is placed in a closed container. On September 14, 2015, put the airtight container with the seeds in the -18℃ to -20℃ refrigeration. Take out th...

Embodiment 2

[0037] 1. Materials and methods:

[0038] 1.1 Research materials:

[0039] On September 4, 2012, fresh and mature seeds of Mallotus nepalensis were collected in Wenshan County, Wenshan Prefecture, Yunnan Province. After collection, they were transported back to the Seed Collection Center of the Southwest Wildlife Germplasm Bank of Kunming Institute of Botany.

[0040] 1.2 Research methods:

[0041] 1.2.1 Drying and refrigeration after refrigeration: The seeds collected in 1.1 are placed in a drying room at 15°C and 15% relative humidity to dry. The Hygrolab C1unit relative humidity meter produced by Rotronic Ltd. is used in conjunction with the Rotronic HC 2-AW probe produced by the same company to measure the relative humidity of the seeds on a regular basis. After the equilibrium relative humidity reaches 15%, it is placed in a closed container. On August 6, 2013, put the airtight container with the seeds in the -18℃ to -20℃ refrigeration. After refrigerating for 3 years and 2 mo...

Embodiment 3

[0053] 1. Materials and methods:

[0054] 1.1 Research materials:

[0055] On October 8, 2009, fresh and mature seeds of Mallotus nepalensis were collected in Yuanjiujia Township, Pu'er Town, Yunnan Province. After collection, they were transported back to the Seed Collection Center of the Southwest Wildlife Germplasm Bank of Kunming Institute of Botany.

[0056] 1.2 Research methods:

[0057] 1.2.1 Drying and refrigeration after refrigeration: The seeds collected in 1.1 are placed in a drying room at 15°C and 15% relative humidity to dry. The Hygrolab C1unit relative humidity meter produced by Rotronic Ltd. is used in conjunction with the Rotronic HC 2-AW probe produced by the same company to measure the relative humidity of the seeds on a regular basis. After the equilibrium relative humidity reaches 15%, it is placed in a closed container. In September 2011, put the airtight container with the seeds in the -18℃ to -20℃ refrigeration. After 5 years of refrigeration, remove the co...

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Abstract

The invention discloses a method for improving the vitality of endosperms of mallotus nepslensis seeds preserved at low temperature. The method comprises the following steps: taking out dry seeds preserved at the low temperature of 18DEG C below zero and 20DEG C below zero together with a closed container for storing the seeds; putting the seeds in an environment with the temperature of 15DEG C and relative humidity of 15 percent for 24 hours, and taking out the seeds; putting the seeds in an incubator with the temperature of 45DEG C, carrying out heat treatment for 6 hours and then taking out the seeds; restoring the temperature of the seeds to room temperature, then putting the seeds on an agar culture medium with the concentration of 1 percent, and putting the agar culture medium in a culturing room with the temperature of 20DEG C for 24 hours; cutting off seed coats and the endosperms of treated seeds, soaking the cut seeds in a 1 percent triphenyltetrazolium chloride (TTC) solution, and putting the mixture under the dark condition of 30DEG C for 48 hours; taking out soaked seeds, cutting the seeds to observe the endosperm dyeing condition, wherein the endosperms with the vitality show uniform red colors. According to the method, the vitality of the endosperms of the mallotus nepslensis seeds which are from different production places and are preserved at low temperature is remarkably improved; the problem that the vitality of the endosperms of the mallotus nepslensis seeds stored at the low temperature is reduced is solved.

Description

Technical field [0001] The invention belongs to the technical field of preservation of plant germplasm resources, and specifically relates to a method for improving the endosperm vitality of wild paulownia seeds after preservation at low temperature (-18°C to -20°C). Background technique [0002] Mallotus nepalensis Müll.Arg. is a deciduous shrub or small tree species of Mallotus Lour. of Euphorbiaceae (Euphorbiaceae). Distributed in mountain slopes or valleys at an altitude of 1700 to 2500 meters in Yunnan, Tibet, and mainly distributed in Bhutan, northeastern India, Myanmar and Nepal abroad. There are few domestic research reports, and foreign reports that its extract has a mild inhibitory effect on the central nervous system. There is no research report on the storage of wild paulownia seeds in Nepal. [0003] Preserving normal seeds at low temperatures (-18°C to -20°C) after drying can greatly extend their lifespan. This has been a consensus internationally, and a number of cr...

Claims

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Application Information

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IPC IPC(8): A01C1/00
CPCA01C1/00
Inventor 杨娟胡枭剑杜燕刘成何华杰杨娅娟李慧杨湘云
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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